Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R2
|
230 |
93.6 |
4701257 |
96.3% |
4527310 |
61.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
734,169 |
C→T |
57.1% |
P268L (CCG→CTG) |
yceG → |
predicted aminodeoxychorismate lyase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 734,169 | 0 | C | T | 57.1%
| 20.2
/ 65.7
| 56 | P268L (CCG→CTG) | yceG | predicted aminodeoxychorismate lyase |
| Reads supporting (aligned to +/- strand): ref base C (10/14); new base T (19/13); total (29/27) |
| Fisher's exact test for biased strand distribution p-value = 2.80e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGAT > minE/734099‑734236
|
gacccgaccGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGcc < 1:690693/71‑1 (MQ=255)
acccgaccGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCg < 1:4587881/71‑1 (MQ=255)
cgaccGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCTGa > 1:2829949/1‑69 (MQ=255)
tGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCTGACAGCGtat < 1:1273130/71‑1 (MQ=255)
tGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCTGACAGCGtat < 1:4698550/71‑1 (MQ=255)
aTTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATaa > 1:2664537/1‑71 (MQ=255)
tACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAacac > 1:3505327/1‑71 (MQ=255)
tACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAacac > 1:1554344/1‑71 (MQ=255)
tACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAaca < 1:3444783/70‑1 (MQ=255)
aCGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAacac < 1:3356899/70‑1 (MQ=255)
cGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAACAcc < 1:4143050/70‑1 (MQ=255)
cGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACAcc < 1:4501887/70‑1 (MQ=255)
gATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCtata > 1:585861/1‑71 (MQ=255)
tGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAACACCTATAcc < 1:1040205/71‑1 (MQ=255)
gCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCg > 1:908610/1‑71 (MQ=255)
cGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGa < 1:4104734/43‑1 (MQ=255)
cGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCgg < 1:4267289/71‑1 (MQ=255)
gTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGt < 1:3383676/71‑1 (MQ=255)
gTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCgg < 1:2715431/70‑1 (MQ=255)
ttATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCg < 1:2514866/46‑1 (MQ=255)
ttATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCta < 1:4022107/57‑1 (MQ=255)
tataATGGCAAACTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAACACCTATACCAtt < 1:1285307/63‑1 (MQ=255)
ataATGGCAAACTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGTCTg > 1:1547959/1‑71 (MQ=255)
taATGGCAAACTTTCTCGTGCAGACCTGGAAACGCTGACAGCg < 1:3368786/43‑1 (MQ=255)
taATGGCAAACTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATaa > 1:2373207/1‑48 (MQ=255)
taATGGCAAACTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGTCTgc > 1:445463/1‑71 (MQ=255)
aaTGGCAAACTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATaa > 1:2268715/1‑47 (MQ=255)
cAAACTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAaca < 1:706478/44‑1 (MQ=255)
aaaCTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAgg > 1:3315368/1‑71 (MQ=255)
aaaCTTTCTCGTGCAGACCTGGAAACGCCGACAGCGtat < 1:4249573/39‑1 (MQ=255)
aaaCTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATaa > 1:4035512/1‑41 (MQ=255)
aaaCTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAacac < 1:2498731/44‑1 (MQ=255)
aaaCTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAgg > 1:1176816/1‑71 (MQ=255)
aaCTTTCTCGTGCAGACCTGGAAACGCTGACAGCg < 1:2437303/35‑1 (MQ=255)
aaCTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGTc < 1:470655/60‑1 (MQ=255)
aaCTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGt > 1:1531795/1‑71 (MQ=255)
aCTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAACACCta > 1:434898/1‑45 (MQ=255)
aCTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGt > 1:966555/1‑70 (MQ=255)
cTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCa > 1:758004/1‑66 (MQ=255)
cTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGc < 1:3748249/71‑1 (MQ=255)
cTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGc > 1:3632077/1‑71 (MQ=255)
cTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAACACATATACCATTACCGGTCTg > 1:2555848/1‑60 (MQ=255)
cGTGCAGACCTGGAAACGCCGACAGCGTATAACACCta < 1:2020773/38‑1 (MQ=255)
gTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAg < 1:1614360/69‑1 (MQ=255)
gACCTGGAAACGCCGACAGCGTATAACACCTATAcc < 1:3471750/36‑1 (MQ=255)
gACCTGGAAACGCCGACAGCGTATAACACCTATACCATTAGCGGTCTGCCGCCAGGTGCGATAGCGACGcc < 1:4379464/71‑1 (MQ=255)
cTGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAg > 1:545697/1‑52 (MQ=255)
cTGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAg > 1:4094906/1‑52 (MQ=255)
cTGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAg > 1:148166/1‑52 (MQ=255)
cTGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCggg > 1:1921048/1‑71 (MQ=255)
cTGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCggg > 1:4021059/1‑71 (MQ=255)
cTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCgc > 1:236956/1‑69 (MQ=255)
tGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCg < 1:491585/56‑1 (MQ=255)
ggAAACGCTGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCggggg > 1:2494966/1‑71 (MQ=255)
aaaCGCCGACAGCGTATAACACCTATACCATTACCTGTCTGCCGCCAg > 1:2170813/1‑48 (MQ=255)
cGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGAt > 1:4561714/1‑71 (MQ=255)
gCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCgggg > 1:1422867/1‑64 (MQ=255)
|
GACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGAT > minE/734099‑734236
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
