Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F21 I0 R1
|
476 |
35.3 |
2812105 |
96.7% |
2719305 |
61.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,225,730 |
G→A |
11.8% |
T117I (ACT→ATT) |
arpA ← |
regulator of acetyl CoA synthetase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,225,730 | 0 | G | A | 11.8%
| 95.2
/ 4.5
| 34 | T117I (ACT→ATT) | arpA | regulator of acetyl CoA synthetase |
| Reads supporting (aligned to +/- strand): ref base G (13/17); new base A (2/2); total (15/19) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 |
CCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGT > W3110S.gb/4225658‑4225798
|
ccATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTA‑‑TGATTTTAGGTTTAATTCTTGTTTATCAagag < 1:1341943/71‑1 (MQ=255)
tAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAgtg > 1:44824/1‑71 (MQ=255)
aaGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAagag > 1:2747605/1‑68 (MQ=255)
aaGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAgtg < 1:295684/70‑1 (MQ=255)
tAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAg > 1:2582263/1‑71 (MQ=255)
tAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAg > 1:1276009/1‑71 (MQ=255)
tAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAg > 1:2088409/1‑71 (MQ=255)
tAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAg > 1:1909988/1‑71 (MQ=255)
tAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAg > 1:1682121/1‑71 (MQ=255)
aTTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGc < 1:1215939/70‑1 (MQ=255)
cTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGtt > 1:243740/1‑70 (MQ=255)
cTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGtt > 1:1342989/1‑70 (MQ=255)
gggAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGa < 1:423490/69‑1 (MQ=255)
aTTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTaa > 1:1575500/1‑70 (MQ=255)
tctttttcttttCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGACGTaaaa > 1:2801069/1‑70 (MQ=255)
ctttttcttttCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAgtgt > 1:2808045/1‑51 (MQ=255)
ctttttcttttCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAAtt > 1:695705/1‑71 (MQ=255)
ttttctATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTaa < 1:2732600/60‑1 (MQ=255)
tATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTc < 1:478793/61‑1 (MQ=255)
tATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTc < 1:1247919/61‑1 (MQ=255)
tGATTTTAGGTTTAATTCTTGTTTATCAAGAgtgt > 1:987467/1‑35 (MQ=255)
tGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTAtttt > 1:1958590/1‑70 (MQ=255)
ttAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCaaa < 1:485286/70‑1 (MQ=255)
ttAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTACATTAGGTATTTTTCaaa < 1:2379593/70‑1 (MQ=255)
tAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCAATTAGGTATTTTTCAAat < 1:1683575/70‑1 (MQ=255)
gTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTc < 1:153987/48‑1 (MQ=255)
tttAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTATGTATTTTTCAAATATTgg < 1:836912/71‑1 (MQ=255)
tttAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAata < 1:2387043/67‑1 (MQ=255)
tttAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTgg < 1:1516535/71‑1 (MQ=255)
tAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTgggg > 1:1418655/1‑71 (MQ=255)
aTTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGc < 1:908283/70‑1 (MQ=255)
cTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCAt < 1:2347073/43‑1 (MQ=255)
ttGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATgg > 1:96934/1‑71 (MQ=255)
gTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGt < 1:2580647/70‑1 (MQ=255)
gTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATgg < 1:829229/69‑1 (MQ=255)
gTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATgg < 1:558176/69‑1 (MQ=255)
tttATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGt < 1:1437893/69‑1 (MQ=255)
ttATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAata > 1:1425020/1‑55 (MQ=255)
tATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGttt > 1:673959/1‑69 (MQ=255)
aaGAGTGTCTGAGCGTTGAGGTAAAATTTACATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTa < 1:1495855/70‑1 (MQ=255)
gagTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAgt < 1:2321299/71‑1 (MQ=255)
|
CCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGT > W3110S.gb/4225658‑4225798
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
