Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F3 I0 R1
|
560 |
56.4 |
4650532 |
94.9% |
4413354 |
59.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,225,730 |
G→A |
18.2% |
T117I (ACT→ATT) |
arpA ← |
regulator of acetyl CoA synthetase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,225,730 | 0 | G | A | 18.2%
| 75.4
/ 10.3
| 33 | T117I (ACT→ATT) | arpA | regulator of acetyl CoA synthetase |
| Reads supporting (aligned to +/- strand): ref base G (10/17); new base A (4/2); total (14/19) |
| Fisher's exact test for biased strand distribution p-value = 3.63e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGT > W3110S.gb/4225660‑4225800
|
aTTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAagag < 1:3833069/71‑1 (MQ=255)
tAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAgtg > 1:1680279/1‑71 (MQ=255)
aaGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAgtg < 1:4205937/70‑1 (MQ=255)
aaGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAgtg > 1:2210338/1‑70 (MQ=255)
aaGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAatg > 1:185875/1‑70 (MQ=255)
tAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAagag > 1:3010937/1‑63 (MQ=255)
tAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAagag > 1:2558912/1‑63 (MQ=255)
tAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAg > 1:3133919/1‑71 (MQ=255)
aaTTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGc > 1:2405584/1‑71 (MQ=255)
gggAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAg < 1:844469/70‑1 (MQ=255)
gggAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAg < 1:3140546/70‑1 (MQ=255)
ttctttttctttTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTaaa < 1:3143697/70‑1 (MQ=255)
tctttttcttttCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAgt < 1:3659895/50‑1 (MQ=255)
tttttcttttCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGa > 1:4583328/1‑61 (MQ=255)
tttttcttttCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAAttt > 1:1931794/1‑71 (MQ=255)
tttttcttttCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAAtt > 1:2116889/1‑70 (MQ=255)
tttcttttctATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTc < 1:548084/70‑1 (MQ=255)
tttcttttctATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTc < 1:3842069/70‑1 (MQ=255)
tttcttttctATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTc > 1:182945/1‑70 (MQ=255)
tcttttctATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCAt > 1:192047/1‑71 (MQ=255)
cttttctATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCAt < 1:737991/70‑1 (MQ=255)
ttctATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCAtt < 1:49222/68‑1 (MQ=255)
tctATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCAtt < 1:43704/67‑1 (MQ=255)
ctATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGt > 1:1051130/1‑70 (MQ=255)
tGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAAttt > 1:997154/1‑56 (MQ=255)
tGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTAt > 1:3255662/1‑67 (MQ=255)
aTTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGTTAAAATTTCCATTAGGTATTTTTc > 1:485497/1‑70 (MQ=255)
ttttAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTATAATTTc < 1:1872/54‑1 (MQ=255)
ttAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCaaa < 1:1692136/70‑1 (MQ=255)
aGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAata < 1:1375199/70‑1 (MQ=255)
aGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAata < 1:4216328/70‑1 (MQ=255)
ggTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATAtt < 1:4190255/71‑1 (MQ=255)
ttAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTc < 1:805279/46‑1 (MQ=255)
ttAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTAt < 1:4449398/56‑1 (MQ=255)
ttAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTggg > 1:2512872/1‑71 (MQ=255)
tAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTgggg > 1:1557502/1‑71 (MQ=255)
ttCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCaaa < 1:2041333/60‑1 (MQ=255)
ttCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCaa < 1:402805/71‑1 (MQ=255)
ttGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCaaa < 1:2793779/57‑1 (MQ=255)
tATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGttt > 1:160537/1‑69 (MQ=255)
aaGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTa < 1:649093/70‑1 (MQ=255)
gtgtCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAgtgt > 1:390931/1‑71 (MQ=255)
|
ATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGT > W3110S.gb/4225660‑4225800
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
