Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F3 I0 R2
|
232 |
76.3 |
4135488 |
90.9% |
3759158 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,747,876 |
G→A |
28.6% |
S44L (TCA→TTA) |
yfiE ← |
predicted DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,747,876 | 0 | G | A | 28.6%
| 77.5
/ 34.9
| 49 | S44L (TCA→TTA) | yfiE | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (8/27); new base A (6/8); total (14/35) |
| Fisher's exact test for biased strand distribution p-value = 1.81e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTA > minE/1747809‑1747926
|
cgtcagcaACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGaa < 1:4029465/67‑1 (MQ=255)
cagcaACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGaa < 1:385568/67‑1 (MQ=255)
cagcaACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACt < 1:1074357/71‑1 (MQ=255)
ttttttCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGaa < 1:880906/60‑1 (MQ=255)
ttttttCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGaa < 1:546619/60‑1 (MQ=255)
ttttttCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGaa < 1:3810701/60‑1 (MQ=255)
ttttttCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGaa < 1:3102781/60‑1 (MQ=255)
gTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGctgctg < 1:3084331/65‑1 (MQ=255)
gTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGctgctg < 1:580049/65‑1 (MQ=255)
aGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGaaa < 1:2808832/71‑1 (MQ=255)
aGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGaa < 1:231899/70‑1 (MQ=255)
aGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAAATCCTGCTCAAGCTGCTGAATATGaaa < 1:1942029/71‑1 (MQ=255)
acaTCCGTCGGCCAATTTTCTCAAATAACTGGACTAAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTc < 1:1882727/71‑1 (MQ=255)
acaTCCGTCGGCCAATTTTCTCAAATAACTGGACTAAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTc < 1:2777339/71‑1 (MQ=255)
acaTCCGTCGGCCAATTTTCTCAAATAACTGGACTAAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTc < 1:817752/71‑1 (MQ=255)
aTCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTc < 1:3108976/69‑1 (MQ=255)
aTCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTc < 1:1289111/69‑1 (MQ=255)
aTCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTc < 1:1965817/69‑1 (MQ=255)
aTCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTc < 1:3970946/69‑1 (MQ=255)
ggCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGa < 1:3955729/70‑1 (MQ=255)
ggCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGa < 1:1855066/70‑1 (MQ=255)
ggCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGa < 1:1416738/70‑1 (MQ=255)
ggCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGa < 1:3239390/70‑1 (MQ=255)
ggCCAATTTACTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGa < 1:952236/70‑1 (MQ=255)
gCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGa < 1:3709257/69‑1 (MQ=255)
ccAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATtg < 1:2457986/71‑1 (MQ=255)
cAATTTTCTCAAATAACTGGACTAAAAACTCCTGCTCAAGCTGCTGAATATg < 1:2650748/52‑1 (MQ=255)
cAATTTTCTCAAATAACTGGACTAAAAACTCCTGCTCAAGCTGCTGAATATg < 1:1487964/52‑1 (MQ=255)
aTTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATtgtg > 1:430156/1‑70 (MQ=255)
aTTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATtgtg > 1:1623502/1‑70 (MQ=255)
aTTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATtgtg > 1:3108859/1‑70 (MQ=255)
aTTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATtgtg > 1:624473/1‑70 (MQ=255)
tttCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTa > 1:1034639/1‑70 (MQ=255)
tttCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTa > 1:1005428/1‑70 (MQ=255)
ttCTCAAATAACTGGACTAAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACTGTCGAt > 1:2262723/1‑63 (MQ=255)
ttCTCAAATAACTGGACTAAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGAt > 1:3890850/1‑63 (MQ=255)
ttCTCAAATAACTGGACTAAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGAt > 1:2913126/1‑63 (MQ=255)
ttCTCAAATAACTGGACTAAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGAt > 1:207916/1‑63 (MQ=255)
ttCTCAAATAACTGGACTAAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGAt > 1:1567877/1‑63 (MQ=255)
ttCTCAAATAACTGGACTAAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGAt > 1:1082828/1‑63 (MQ=255)
tAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCAc > 1:304682/1‑48 (MQ=255)
tAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCAc > 1:1212474/1‑48 (MQ=255)
aCTAAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATtgtg < 1:3454320/52‑1 (MQ=255)
aCTAAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATtgtg < 1:3010383/52‑1 (MQ=255)
aCTAAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATtgtg < 1:1151017/52‑1 (MQ=255)
tGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTc < 1:3972837/37‑1 (MQ=255)
tGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTc < 1:2186239/37‑1 (MQ=255)
tGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTc < 1:4058469/37‑1 (MQ=255)
tGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTc < 1:2181401/37‑1 (MQ=255)
|
CGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTA > minE/1747809‑1747926
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
