Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,940,785 |
T→C |
K473K (AAA→AAG) |
bcsG ← |
predicted inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,940,785 | 0 | T | C | 100.0%
| 113.4
/ NA
| 34 | K473K (AAA→AAG) | bcsG | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/20); total (14/20) |
GCCAGGAAGCTGCTCGGTTGTTCGATGACAATCGGTGCCCCCTGATGCGGTGCCTTCATGCCGAAGAATTTCACCCCAACGGGGACGTCGGTGATAGACGGGCTAGGGATATCACGTAGGCCAGATACCTGCATTC > W3110S.gb/3940717‑3940852
|
gCCAGGAAGCTGCTCGGTTGTTCGATGACAATCGGTGCCCCCTGATGCGGTGCCTTCATGCCGAAGAActt < 1:451172/71‑1 (MQ=255)
gCCAGGAAGCTGCTCGGTTGTTCGATGACAATCGGTGCCCCCTGATGCGGTGCCTTCATGCCGAAGAActt < 1:2531307/71‑1 (MQ=255)
aGCTGCTCGGTTGTTCGATGACAATCGGTGCCCCCTGATGCGGTGCCTTCATGCCGAAGAACTTCAcccc < 1:3774016/70‑1 (MQ=255)
aGCTGCTCGGTTGTTCGATGACAATCGGTGCCCCCTGATGCGGTGCCTTCATGCCGAAGAACTTCAcccc > 1:1464934/1‑70 (MQ=255)
gctCGGTTGTTCGATGACAATCGGTGCCCCCTGATGCGGTGCCTTCATGCCGAAGAACTTCACCCCAACgg > 1:4352019/1‑71 (MQ=255)
ctCGGTTGTTCGATGACAATCGGTGCCCCCTGATGCGGTGCCTTCATGCCGAAGAACTTCACCCCAACggg < 1:1127066/71‑1 (MQ=255)
ctCGGTTGTTCGATGACAATCGGTGCCCCCTGATGCGGTGCCTTCATGCCGAAGAACTTCACCCCAACggg < 1:4341154/71‑1 (MQ=255)
ctCGGTTGTTCGATGACAATCGGTGCCCCCTGATGCGGTGCCTTCATGCCGAAGAACTTCACCCCAACggg < 1:2403501/71‑1 (MQ=255)
cGATGACAATCGGTGCCCCCTGATGCGGTGCCTTCATGCCGAAGAACTTCACCCCAACGGGGACGTCGGt < 1:2031149/70‑1 (MQ=255)
tGACAATCGGTGCCCCCTGATGCGGTGCCTTCATGCCGAAGAACTTCACCCCAACGGGGACGTCGGTGATa < 1:4085866/71‑1 (MQ=255)
tGACAATCGGTGCCCCCTGATGCGGTGCCTTCATGCCGAAGAACTTCACCCCAACGGGGACGTCGGTGATa < 1:3448162/71‑1 (MQ=255)
tGACAATCGGTGCCCCCTGATGCGGTGCCTTCATGCCGAAGAACTTCACCCCAACGGGGACGTCGGTGATa < 1:1758168/71‑1 (MQ=255)
tGACAATCGGTGCCCCCTGATGCGGTGCCTTCATGCCGAAGAACTTCACCCCAACGGGGACGTCGGTGATa < 1:1730739/71‑1 (MQ=255)
ggTGCCCCCTGATGCGGGGCCTTCATGCCGAAGAACTTCACCCCAACGGGGACGTCGGTGATa < 1:1812384/63‑1 (MQ=255)
gTGCCCCCTGATGCGGTGCCTTCATGCCGAAGAACTTCACCCCAACGGGGACGTCGGTGATAGACGGGCt < 1:3390352/70‑1 (MQ=255)
tGCCCCCTGATGCGGTGCCTTCATGCCGAAGAACTTCACCCCAACGGGGACGTCGGTGATAGACGGGCTAg > 1:766369/1‑71 (MQ=255)
cccTGATGCGGTGCCTTCATGCCGAAGAACTTCACCCCAACGGGGACGTCGGTGATAGACGGGCTAGGGat < 1:364884/71‑1 (MQ=255)
cTGATGCGGTGCCTTCATGCCGAAGAACTTCACCCCAACggg < 1:2174514/42‑1 (MQ=255)
tGATGCGGTGCCTTCATGCCGAAGAACTTCACCCCAACGGGGACGTCGGTGATAGACGGGCTAGGGatat > 1:1026399/1‑70 (MQ=255)
gATGCGGTGCCTTCATGCCGAAGAACTTCACCCCAACAGGGACGTCGGTGATAGACGGGCTAGGGATATCa > 1:730757/1‑71 (MQ=255)
ccTTCATGCCGAAGAACTTCACCCCAACGGGGACGTCGGTGATAGACGGGCTAGGGATATCACGTAGGc > 1:3088565/1‑69 (MQ=255)
ttCATGCCGAAGAACTTCACCCCAACGGGGACGTCGGt < 1:4613343/38‑1 (MQ=255)
ttCATGCCGAAGAACTTCACCCCAACGGGGACGTCGGTGATa < 1:2329289/42‑1 (MQ=255)
ttCATGCCGAAGAACTTCACCCCAACGGGGACGTCGGTGATAGACGGGCTAGGGat < 1:86500/56‑1 (MQ=255)
cATGCCGAAGAACTTCACCCCAACGGGGACGTCGGTGATAGACGGGCTAGGGATATCACGTAGGCCAGa < 1:2839390/69‑1 (MQ=255)
cATGCCGAAGAACTTCACCCCAACGGGGACGTCGGTGATAGACGGGCTAGGGATATCACGTAGGCCAGAt < 1:992932/70‑1 (MQ=255)
cATGCCGAAGAACTTCACCCCAACGGGGACGTCGGTGATAGACGGGCTAGGGATATCACGTAGGCCAGAt < 1:1745245/70‑1 (MQ=255)
cATGCCGAAGAACTTCACCCCAACGGGGACGTCGGTGATAGACGGGCTAGGGATATCACGTAGGCCAGATa > 1:282798/1‑71 (MQ=255)
cATGCCGAAGAACTTCACCCCAACGGGGACGTCGGTGATAGACGGGCTAGGGATATCACGTAGGCCAGATa > 1:255296/1‑71 (MQ=255)
cATGCCGAAGAACTTCACCCCAACGGGGACGTCGGTGATAGACGGGCTAGGGATATCACGTAGGCCAGATa > 1:1772029/1‑71 (MQ=255)
cATGCCGAAGAACTTCACCCCAACGGGGACGTCGGTGATAGACGGGCTAGGGATATCACGTAGGCCAGATa > 1:1415376/1‑71 (MQ=255)
cATGCCGAAGAACTTCACCCCAACGGGGACGTCGGTGATAGACGGGCTAGGGATATCACGTAGGCCAGATa > 1:872677/1‑71 (MQ=255)
cATGCCGAAGAACTTCACCCAAACGGGGACGTCGGTGATAGACGGGCTAGGGATATCACGTAGGCCAGATa > 1:3160733/1‑71 (MQ=255)
ccGAAGAACTTCACCCCAACGGGGACGTCGGTGATAGACGGGCTAGGGATATCACGTa > 1:1110011/1‑58 (MQ=255)
ccGAAGAACTTCACCCCAACGGGGACGTCGGTGATAGACGGGCTAGGGATATCACGTAGGCCAGATACCTg < 1:4280198/71‑1 (MQ=255)
gaaCTTCACCCCAACGGGGACGTCGGTGATAGACGGGCTAGGGATATCACGTAGGCCAGATACCTGCATTc > 1:2759828/1‑71 (MQ=255)
|
GCCAGGAAGCTGCTCGGTTGTTCGATGACAATCGGTGCCCCCTGATGCGGTGCCTTCATGCCGAAGAATTTCACCCCAACGGGGACGTCGGTGATAGACGGGCTAGGGATATCACGTAGGCCAGATACCTGCATTC > W3110S.gb/3940717‑3940852
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
