Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F8 I0 R1
|
311 |
50.1 |
4085234 |
95.9% |
3917739 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,125,813 |
C→T |
35.3% |
P152L (CCT→CTT) |
rpe → |
D‑ribulose‑5‑phosphate 3‑epimerase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,125,813 | 0 | C | T | 35.3%
| 74.0
/ 65.5
| 68 | P152L (CCT→CTT) | rpe | D‑ribulose‑5‑phosphate 3‑epimerase |
| Reads supporting (aligned to +/- strand): ref base C (26/18); new base T (13/11); total (39/29) |
| Fisher's exact test for biased strand distribution p-value = 7.99e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATGGATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAG > W3110S.gb/4125746‑4125881
|
aTGGATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCAttcttc > 1:1006326/1‑70 (MQ=255)
aTGGATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCTTCa < 1:150102/71‑1 (MQ=255)
aTGGATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTc > 1:4040971/1‑70 (MQ=255)
gATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCTTCaaa < 1:1059307/70‑1 (MQ=255)
gATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCTTCaaa > 1:1671282/1‑70 (MQ=255)
aTAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAaca < 1:3141587/71‑1 (MQ=255)
tAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCTTCAAaca < 1:3388535/70‑1 (MQ=255)
aaGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACt > 1:414135/1‑71 (MQ=255)
gATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCTTCAAACACTGGATa > 1:2248215/1‑70 (MQ=255)
gATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAaca > 1:2910649/1‑63 (MQ=255)
gATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAaca > 1:3936247/1‑63 (MQ=255)
gATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATaa < 1:3853747/71‑1 (MQ=255)
gATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATa < 1:3709870/70‑1 (MQ=255)
tgATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATaaa > 1:241813/1‑68 (MQ=255)
tgATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTg > 1:2347721/1‑71 (MQ=255)
ctgctgATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCTTCAAacac < 1:492376/55‑1 (MQ=255)
ctgctgATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCTTCAAACACTGGATAAACTGcgcg < 1:2043203/70‑1 (MQ=255)
ctgATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAg < 1:3227241/70‑1 (MQ=255)
tgATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTa > 1:822912/1‑71 (MQ=255)
gTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTc > 1:217383/1‑71 (MQ=255)
gTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCaaa > 1:3187313/1‑44 (MQ=255)
gTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCaaa > 1:651708/1‑44 (MQ=255)
cGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGcc > 1:623965/1‑71 (MQ=255)
cGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGcc > 1:2965065/1‑71 (MQ=255)
gTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATa < 1:2153402/49‑1 (MQ=255)
gTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATa < 1:1877801/49‑1 (MQ=255)
gTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATa < 1:1474222/49‑1 (MQ=255)
tCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGt < 1:3093418/71‑1 (MQ=255)
tCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGt < 1:11781/71‑1 (MQ=255)
aaCCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTAt > 1:746538/1‑71 (MQ=255)
aaCCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTAt > 1:2591666/1‑71 (MQ=255)
cccTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATcg < 1:2807194/71‑1 (MQ=255)
cTGGTTTCGGCGGTCAGTCTTTCATTCTTCAAaca > 1:497168/1‑35 (MQ=255)
cTGGTTTCGGCGGTCAGTCTTTCATTCTTCAAACACt > 1:1593583/1‑37 (MQ=255)
cTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATcgac < 1:1093850/71‑1 (MQ=255)
cTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATcgac < 1:1559391/71‑1 (MQ=255)
cTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATcgac < 1:3987414/71‑1 (MQ=255)
tGGTTTCGGCGGTCAGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATcgac > 1:3666950/1‑70 (MQ=255)
tGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATa < 1:1970680/41‑1 (MQ=255)
tGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACAATGGATa < 1:527325/41‑1 (MQ=255)
ggTTTCGGCGGTCAGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATcgac > 1:3196070/1‑69 (MQ=255)
tttCGGCGGTCAGTCTTTCATTCTTCAAACACTGGATAAACTGcgcg < 1:3230591/47‑1 (MQ=255)
tttCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATcgacga < 1:3008927/69‑1 (MQ=255)
ttCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTc > 1:511992/1‑71 (MQ=255)
tCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATa < 1:969552/36‑1 (MQ=255)
ggTCAGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCtt < 1:3467618/71‑1 (MQ=255)
tCAGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTg < 1:514640/71‑1 (MQ=255)
aGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGa > 1:3186658/1‑70 (MQ=255)
aGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGa < 1:299288/70‑1 (MQ=255)
aGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGa > 1:1593375/1‑70 (MQ=255)
aGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGa > 1:692075/1‑70 (MQ=255)
gTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAg < 1:1431271/38‑1 (MQ=255)
gTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAg < 1:2642473/38‑1 (MQ=255)
tCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACAt < 1:1822350/71‑1 (MQ=255)
cTTTCATTCCTCAAACACTGGATAAACTGCGCGAAg > 1:2970608/1‑36 (MQ=255)
cTTTCATTCCTCAAACACTGGATAAACTGCGCGAAg > 1:1868758/1‑36 (MQ=255)
cTTTCATTCCTCAAACACTGGATAAACTGCGCGAAg > 1:1745166/1‑36 (MQ=255)
cTTTCATTCCTCAAACACTGGATAAACTGCGCGAAg > 1:1595194/1‑36 (MQ=255)
cTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACAtt > 1:1734715/1‑71 (MQ=255)
tttCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACAtt > 1:262493/1‑70 (MQ=255)
tttCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTc > 1:524103/1‑71 (MQ=255)
tttCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTg > 1:1096742/1‑59 (MQ=255)
tttCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTg > 1:1930719/1‑59 (MQ=255)
tttCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTg > 1:1432677/1‑59 (MQ=255)
tttCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTc > 1:161220/1‑71 (MQ=255)
tCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACa > 1:3459135/1‑66 (MQ=255)
tCATTCCTCAAACACTGGATAAACTGCGCGAAGTa > 1:412353/1‑35 (MQ=255)
tCATTCCTCAAACACTGGATAAACTGCGCGAAGTa > 1:975183/1‑35 (MQ=255)
tCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGa > 1:1842880/1‑71 (MQ=255)
ttcttcAAACACTGGATAAACTGCGCGAAGTACGTc < 1:62488/36‑1 (MQ=255)
ttcttcAAACACTGGATAAACTGCGCGAAGTACGTc < 1:813500/36‑1 (MQ=255)
ccTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAg < 1:1210677/70‑1 (MQ=255)
|
ATGGATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAG > W3110S.gb/4125746‑4125881
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
