Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 479,895 A→G 41.2% V133V (GTA→GTG)  cydB → cytochrome d terminal oxidase, subunit II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE479,8950AG41.2% 41.2 / 84.2 114V133V (GTA→GTG) cydBcytochrome d terminal oxidase, subunit II
Reads supporting (aligned to +/- strand):  ref base A (46/21);  new base G (31/16);  total (77/37)
Fisher's exact test for biased strand distribution p-value = 8.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01

Only 100 of 116 total aligned reads displayed.

GAAACCCGCTGGCGTAACATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTG  >  minE/479830‑479961
                                                                 |                                                                  
gaaACCCGCTGGCGTAACATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAAtt                                                                 >  1:1579618/1‑69 (MQ=255)
gaaACCCGCTGGCGTAACATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAAtt                                                                 >  1:1779296/1‑69 (MQ=255)
     ccGCTGGCGTAACATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGgtgt                                                            >  1:1733034/1‑69 (MQ=255)
     ccGCTGGCGTAACATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGgtgt                                                            >  1:2883090/1‑69 (MQ=255)
     ccGCTGGCGTAACATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGgtgt                                                            >  1:2886636/1‑69 (MQ=255)
     ccGCTGGCGTAACATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGgtgt                                                            >  1:149293/1‑69 (MQ=255)
     ccGCTGGCGTAACATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGgtgt                                                            >  1:296681/1‑69 (MQ=255)
     ccGCTGGCGTAACATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGgtgt                                                            >  1:1794471/1‑69 (MQ=255)
     ccGCTGGCGTAACATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGgtgt                                                            >  1:2252609/1‑69 (MQ=255)
     ccGCTGGCGTAACATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGgtgt                                                            >  1:1634548/1‑69 (MQ=255)
     ccGCTGGCGTAACATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGgtgt                                                            >  1:876966/1‑69 (MQ=255)
     ccGCTGGCGTAACATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGgtgt                                                            >  1:947643/1‑69 (MQ=255)
     ccGCTGGCGTAACATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGgtgt                                                            >  1:1155355/1‑69 (MQ=255)
     ccGCTGGCGTAACATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGgtgt                                                            >  1:1117549/1‑69 (MQ=255)
     ccGCTGGCGTAACATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGgtg                                                             >  1:3365159/1‑68 (MQ=255)
     ccGCTGGCGTAACATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGgtg                                                             >  1:2931188/1‑68 (MQ=255)
     ccGCTGGCGTAACATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGgtg                                                             >  1:2912134/1‑68 (MQ=255)
     ccGCTGGCGTAACATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTACGCCGCTGGTGATTGgtg                                                             >  1:527876/1‑68 (MQ=255)
               aaCATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGGTGTAGCGTTCgg                                                   >  1:2679841/1‑68 (MQ=255)
               aaCATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGGTGTAGCGTTCgg                                                   >  1:2990080/1‑68 (MQ=255)
               aaCATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGGTGTAGCGTTCGGt                                                  >  1:2382754/1‑69 (MQ=255)
               aaCATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGGTGTAGCGTTCGGt                                                  >  1:948545/1‑69 (MQ=255)
               aaCATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGGTGTAGCGTTCGGt                                                  >  1:199528/1‑69 (MQ=255)
               aaCATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGGTGTAGCGTTCGGt                                                  >  1:2548073/1‑69 (MQ=255)
               aaCATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGGTGTAGCGTTCGGt                                                  >  1:2140769/1‑69 (MQ=255)
               aaCATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGGTGTAGCGTTCGGt                                                  >  1:2633910/1‑69 (MQ=255)
               aaCATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGGTGTAGCGTTCGGt                                                  >  1:1505122/1‑69 (MQ=255)
               aaCATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGGTGTAGCGTTCGGt                                                  >  1:753574/1‑69 (MQ=255)
               aaCATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGGTGTAGCGTTCGGt                                                  >  1:784729/1‑69 (MQ=255)
               aaCATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGGTGTAGCGTTCGGt                                                  >  1:790434/1‑69 (MQ=255)
                aCATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTa                                                 >  1:1219216/1‑69 (MQ=255)
                aCATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTa                                                 >  1:2104117/1‑69 (MQ=255)
                aCATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTa                                                 <  1:2173610/69‑1 (MQ=255)
                  aTGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGGGTTCGGTaa                                                <  1:2656687/68‑1 (MQ=255)
                     tgGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGGTGTAGCGTTCGGTAACCt                                             <  1:825539/68‑1 (MQ=255)
                     tgGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGt                                                       >  1:2753705/1‑58 (MQ=255)
                     tgGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTg                                            >  1:3372584/1‑69 (MQ=255)
                     tgGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTg                                            >  1:3239537/1‑69 (MQ=255)
                     tgGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTg                                            >  1:3402205/1‑69 (MQ=255)
                     tgGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTg                                            >  1:129920/1‑69 (MQ=255)
                     tgGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTg                                            >  1:550193/1‑69 (MQ=255)
                     tgGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTg                                            >  1:57588/1‑69 (MQ=255)
                     tgGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTg                                            >  1:2778738/1‑69 (MQ=255)
                     tgGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTg                                            >  1:1576372/1‑69 (MQ=255)
                     tgGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTg                                            >  1:2658433/1‑69 (MQ=255)
                     tgGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTg                                            >  1:1157418/1‑69 (MQ=255)
                     tgGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTg                                            >  1:1076146/1‑69 (MQ=255)
                      gggACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGGTGTAGCGTTCGGTAACCt                                             <  1:2412362/67‑1 (MQ=255)
                      gggACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGGTGTAGCGTTCGGTAACCt                                             <  1:2361291/67‑1 (MQ=255)
                      gggACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGGTGTAGCGTTCGGTAACCt                                             <  1:1503251/67‑1 (MQ=255)
                      gggACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGGTGTAGCGTTCGGTAACCt                                             <  1:3421391/67‑1 (MQ=255)
                      gggACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGGTGTAGCGTTCGGTAACCt                                             <  1:2803307/67‑1 (MQ=255)
                      gggACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGGTGTAGCGTTCGGTAACCt                                             <  1:832578/67‑1 (MQ=255)
                      gggACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGGTGTAGCGTTCGGTAACCt                                             <  1:1777594/67‑1 (MQ=255)
                      gggACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGGTGTAGCGTTCGGTAACCt                                             <  1:3420732/67‑1 (MQ=255)
                      gggACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGGTGTAGCGTTCGGTAACCt                                             <  1:2704769/67‑1 (MQ=255)
                      gggACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGGTGTAGCGTTCGGTAACCt                                             <  1:1257035/67‑1 (MQ=255)
                      gggACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGGTGTAGCGTTCGGTAACCt                                             <  1:2632623/67‑1 (MQ=255)
                      gggACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGGTGTAGCGTTCGGTAACCt                                             <  1:2619467/67‑1 (MQ=255)
                      gggACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGGTGTAGCGTTCGGTAACCt                                             <  1:1298105/67‑1 (MQ=255)
                       tgACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGt                                           <  1:1468187/67‑1 (MQ=255)
                       ggACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGt                                           <  1:1548568/68‑1 (MQ=255)
                       ggACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGt                                           <  1:3414530/68‑1 (MQ=255)
                       ggACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGt                                           <  1:2145288/68‑1 (MQ=255)
                       ggACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGt                                           >  1:358572/1‑68 (MQ=255)
                       ggACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGt                                           <  1:391401/68‑1 (MQ=255)
                       ggACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGt                                           >  1:1468111/1‑68 (MQ=255)
                       ggACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGt                                           <  1:242491/68‑1 (MQ=255)
                       ggACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGt                                           >  1:1711131/1‑68 (MQ=255)
                         aCTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTGATTGGTGTAGCGTTCGGTAACCt                                             <  1:575752/64‑1 (MQ=255)
                              ggCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAggg                                    >  1:725994/1‑68 (MQ=255)
                              ggCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAggg                                    >  1:32961/1‑68 (MQ=255)
                              ggCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAgg                                     >  1:2719345/1‑67 (MQ=255)
                              ggCATCTTCATTGGTAGCTTCGTTCAGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAggg                                    >  1:2616811/1‑68 (MQ=255)
                                cATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGt                                 <  1:534957/69‑1 (MQ=255)
                                cATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGt                                 <  1:1934372/69‑1 (MQ=255)
                                cATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGt                                 <  1:3218231/69‑1 (MQ=255)
                                    ttCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTa                                                           >  1:1274412/1‑39 (MQ=255)
                                    ttCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTa                                                 <  1:259903/49‑1 (MQ=255)
                                    ttCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGc                                        <  1:3040501/58‑1 (MQ=255)
                                    ttCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGt                                 >  1:2873354/1‑65 (MQ=255)
                                    ttCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGt                                 >  1:891067/1‑65 (MQ=255)
                                    ttCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGt                                 >  1:1206497/1‑65 (MQ=255)
                                    ttCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGt                                 >  1:2369771/1‑65 (MQ=255)
                                    ttCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGt                                 >  1:1761585/1‑65 (MQ=255)
                                    ttCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGt                                 >  1:2617311/1‑65 (MQ=255)
                                    ttCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGt                                 >  1:1337353/1‑65 (MQ=255)
                                    ttCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGt                                 >  1:1338092/1‑65 (MQ=255)
                                    ttCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGt                                 >  1:3070434/1‑65 (MQ=255)
                                    ttCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGt                                 >  1:3165165/1‑65 (MQ=255)
                                    ttCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTAcc                              >  1:1491239/1‑68 (MQ=255)
                                    ttCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTAcc                              >  1:3216950/1‑68 (MQ=255)
                                    ttCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTAcc                              >  1:225854/1‑68 (MQ=255)
                                    ttCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCg                             >  1:1004155/1‑69 (MQ=255)
                                    ttCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCg                             >  1:3118297/1‑69 (MQ=255)
                                        ttGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTc                          <  1:2799405/68‑1 (MQ=255)
                                        ttGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTc                          <  1:3179815/68‑1 (MQ=255)
                                        ttGGTAGCTTCGTTCCGCCGCTGCTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTc                          <  1:431385/68‑1 (MQ=255)
                                                                 aaTTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTg  <  1:430187/67‑1 (MQ=255)
                                                                 aaTTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTg  <  1:2265359/67‑1 (MQ=255)
                                                                 |                                                                  
GAAACCCGCTGGCGTAACATGTGGGACTGGGGCATCTTCATTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTG  >  minE/479830‑479961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A