Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 761,731 A→C 21.3% P118P (CCT→CCG)  potD ← polyamine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE761,7310AC21.3% 160.9 / 45.4 103P118P (CCT→CCG) potDpolyamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (51/30);  new base C (12/10);  total (63/40)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 103 total aligned reads displayed.

ACCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTC  >  minE/761665‑761787
                                                                  |                                                        
aCCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAgg                                                        >  1:1020632/1‑69 (MQ=255)
aCCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAgg                                                        >  1:1824720/1‑69 (MQ=255)
aCCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAgg                                                        <  1:1951475/69‑1 (MQ=255)
aCCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAgg                                                        >  1:2217423/1‑69 (MQ=255)
aCCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAgg                                                        >  1:2693700/1‑69 (MQ=255)
aCCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAgg                                                        >  1:2977421/1‑69 (MQ=255)
aCCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAgg                                                        >  1:3222355/1‑69 (MQ=255)
aCCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAgg                                                        >  1:3363082/1‑69 (MQ=255)
aCCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAgg                                                        >  1:1226171/1‑69 (MQ=255)
aCCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAgg                                                        >  1:359420/1‑69 (MQ=255)
aCCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAgg                                                        >  1:385517/1‑69 (MQ=255)
  cGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCt                                                      <  1:3101056/69‑1 (MQ=255)
  cGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCt                                                      <  1:2456086/69‑1 (MQ=255)
  cGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCt                                                      <  1:447429/69‑1 (MQ=255)
  cGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCt                                                      <  1:2145828/69‑1 (MQ=255)
  cGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCt                                                      <  1:2095129/69‑1 (MQ=255)
  cGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCt                                                      <  1:203923/69‑1 (MQ=255)
  cGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCt                                                      <  1:582004/69‑1 (MQ=255)
  cGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCt                                                      <  1:897817/69‑1 (MQ=255)
                  cGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCt                                                      <  1:1972104/53‑1 (MQ=255)
                        aCCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCga                                 <  1:2720457/68‑1 (MQ=255)
                                tataCGGAATGGAGTAGTCGTTATTCGGGTCAAAAGTCtt                                                     >  1:1639143/1‑40 (MQ=255)
                                tataCGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCtt                                                     >  1:843505/1‑40 (MQ=255)
                                tataCGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCtt                                                     >  1:1331277/1‑40 (MQ=255)
                                tataCGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCtt                                                     >  1:620532/1‑40 (MQ=255)
                                tataCGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCtt                                                     >  1:1939441/1‑40 (MQ=255)
                                tataCGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCtt                                                     >  1:3402338/1‑40 (MQ=255)
                                tataCGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCt                                                      >  1:2421497/1‑39 (MQ=255)
                                 ataCGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGaa                       >  1:2326747/1‑69 (MQ=255)
                                    cGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGc                           >  1:2847574/1‑62 (MQ=255)
                                    cGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGc                           >  1:2105395/1‑62 (MQ=255)
                                    cGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGtt                    >  1:1494955/1‑69 (MQ=255)
                                    cGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGtt                    >  1:2303452/1‑69 (MQ=255)
                                    cGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGtt                    >  1:2861902/1‑69 (MQ=255)
                                    cGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGtt                    >  1:2657949/1‑69 (MQ=255)
                                    cGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGtt                    >  1:165339/1‑69 (MQ=255)
                                    cGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGtt                    >  1:3119929/1‑69 (MQ=255)
                                    cGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGtt                    >  1:209892/1‑69 (MQ=255)
                                    cGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGtt                    >  1:1732132/1‑69 (MQ=255)
                                    cGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGtt                    >  1:3299474/1‑69 (MQ=255)
                                    cGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGt                     >  1:628967/1‑68 (MQ=255)
                                    cGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTATGGATCGAGATTGCTGAAGtt                    >  1:360349/1‑69 (MQ=255)
                                    cGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGACTGGATCGAGATTGCTGAAGtt                    >  1:3422211/1‑69 (MQ=255)
                                              tagtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTc          <  1:3026476/69‑1 (MQ=255)
                                              tagtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTc          <  1:1317799/69‑1 (MQ=255)
                                              tagtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTc          <  1:1368355/69‑1 (MQ=255)
                                                gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa        >  1:825383/1‑69 (MQ=255)
                                                gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa        <  1:3168255/69‑1 (MQ=255)
                                                gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa        <  1:2925035/69‑1 (MQ=255)
                                                gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa        >  1:3027859/1‑69 (MQ=255)
                                                gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa        <  1:1278815/69‑1 (MQ=255)
                                                gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa        >  1:2584461/1‑69 (MQ=255)
                                                gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa        <  1:623926/69‑1 (MQ=255)
                                                gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa        <  1:1102860/69‑1 (MQ=255)
                                                gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa        <  1:1164297/69‑1 (MQ=255)
                                                gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa        >  1:2537871/1‑69 (MQ=255)
                                                gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa        <  1:2482938/69‑1 (MQ=255)
                                                gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa        >  1:1710143/1‑69 (MQ=255)
                                                gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa        >  1:2224168/1‑69 (MQ=255)
                                                gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa        >  1:1387812/1‑69 (MQ=255)
                                                gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa        >  1:870117/1‑69 (MQ=255)
                                                gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa        >  1:1383924/1‑69 (MQ=255)
                                                gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa        >  1:2602920/1‑69 (MQ=255)
                                                gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa        >  1:1750513/1‑69 (MQ=255)
                                                gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa        <  1:2189237/69‑1 (MQ=255)
                                                gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAACTTTGTTAACTTCGa        >  1:47423/1‑69 (MQ=255)
                                                gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCT‑GATCGAGATTGCTGAAGTTTGTTAACtt           >  1:1233588/1‑65 (MQ=255)
                                                   gTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCgag                                <  1:104149/42‑1 (MQ=255)
                                                   gTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCgag                                <  1:2013745/42‑1 (MQ=255)
                                                   gTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCgag                                <  1:309699/42‑1 (MQ=255)
                                                   gTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCgag                                <  1:2164594/42‑1 (MQ=255)
                                                   gTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCgag                                <  1:2181288/42‑1 (MQ=255)
                                                   gTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCgag                                <  1:2202579/42‑1 (MQ=255)
                                                   gTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCgag                                <  1:2906044/42‑1 (MQ=255)
                                                   gTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACtt     >  1:2937574/1‑69 (MQ=255)
                                                     tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg    >  1:2158540/1‑68 (MQ=255)
                                                     tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg    >  1:1738296/1‑68 (MQ=255)
                                                     tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg    >  1:1608403/1‑68 (MQ=255)
                                                     tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg    >  1:1479742/1‑68 (MQ=255)
                                                     tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg    >  1:3281035/1‑68 (MQ=255)
                                                     tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg    >  1:3247060/1‑68 (MQ=255)
                                                     tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg    >  1:2109199/1‑68 (MQ=255)
                                                     tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg    >  1:2975767/1‑68 (MQ=255)
                                                     tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg    >  1:2941695/1‑68 (MQ=255)
                                                     tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg    >  1:8046/1‑68 (MQ=255)
                                                     tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg    >  1:2369511/1‑68 (MQ=255)
                                                     tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg    >  1:1358238/1‑68 (MQ=255)
                                                      aTTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc  <  1:1597225/69‑1 (MQ=255)
                                                      aTTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc  <  1:1020402/69‑1 (MQ=255)
                                                      aTTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc  <  1:1189094/69‑1 (MQ=255)
                                                      aTTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc  <  1:3434855/69‑1 (MQ=255)
                                                      aTTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc  <  1:3424297/69‑1 (MQ=255)
                                                      aTTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc  <  1:3282052/69‑1 (MQ=255)
                                                      aTTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc  <  1:2913467/69‑1 (MQ=255)
                                                      aTTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc  <  1:2802177/69‑1 (MQ=255)
                                                      aTTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc  <  1:2377084/69‑1 (MQ=255)
                                                      aTTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc  <  1:1513831/69‑1 (MQ=255)
                                                      aTTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTc  >  1:367988/1‑69 (MQ=255)
                                                      aTTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTc  >  1:1262362/1‑69 (MQ=255)
                                                      aTTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTc  >  1:1837535/1‑69 (MQ=255)
                                                                  |                                                        
ACCGTTAACACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTC  >  minE/761665‑761787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A