Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 761,771 G→C 23.0% T105R (ACA→AGA) ‡ potD ← polyamine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE761,7710GC23.0% 171.3 / 47.2 100T105R (ACA→AGA) ‡potDpolyamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (19/58);  new base C (12/11);  total (31/69)
Fisher's exact test for biased strand distribution p-value = 1.97e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01

TAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCAACGTAA  >  minE/761711‑761830
                                                            |                                                           
tagtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTc                                                     <  1:3026476/69‑1 (MQ=255)
tagtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTc                                                     <  1:1317799/69‑1 (MQ=255)
tagtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTc                                                     <  1:1368355/69‑1 (MQ=255)
  gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                                   >  1:2224168/1‑69 (MQ=255)
  gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                                   <  1:2189237/69‑1 (MQ=255)
  gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                                   <  1:2482938/69‑1 (MQ=255)
  gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                                   >  1:2537871/1‑69 (MQ=255)
  gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                                   >  1:2584461/1‑69 (MQ=255)
  gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                                   >  1:2602920/1‑69 (MQ=255)
  gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                                   >  1:1750513/1‑69 (MQ=255)
  gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                                   >  1:1710143/1‑69 (MQ=255)
  gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                                   <  1:3168255/69‑1 (MQ=255)
  gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                                   <  1:623926/69‑1 (MQ=255)
  gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                                   <  1:2925035/69‑1 (MQ=255)
  gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                                   >  1:1387812/1‑69 (MQ=255)
  gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                                   >  1:1383924/1‑69 (MQ=255)
  gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                                   >  1:825383/1‑69 (MQ=255)
  gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                                   <  1:1102860/69‑1 (MQ=255)
  gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                                   >  1:908845/1‑69 (MQ=255)
  gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                                   <  1:1164297/69‑1 (MQ=255)
  gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                                   >  1:2955516/1‑69 (MQ=255)
  gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                                   >  1:3027859/1‑69 (MQ=255)
  gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                                   <  1:1278815/69‑1 (MQ=255)
  gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGa                                                   >  1:870117/1‑69 (MQ=255)
  gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAACTTTGTTAACTTCGa                                                   >  1:47423/1‑69 (MQ=255)
  gtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCT‑GATCGAGATTGCTGAAGTTTGTTAACtt                                                      >  1:1233588/1‑65 (MQ=255)
     gTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACtt                                                >  1:2937574/1‑69 (MQ=255)
       tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg                                               >  1:2109199/1‑68 (MQ=255)
       tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg                                               >  1:2158540/1‑68 (MQ=255)
       tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg                                               >  1:2369511/1‑68 (MQ=255)
       tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg                                               >  1:8046/1‑68 (MQ=255)
       tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg                                               >  1:3281035/1‑68 (MQ=255)
       tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg                                               >  1:2975767/1‑68 (MQ=255)
       tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg                                               >  1:3247060/1‑68 (MQ=255)
       tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg                                               >  1:1479742/1‑68 (MQ=255)
       tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg                                               >  1:1738296/1‑68 (MQ=255)
       tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg                                               >  1:2941695/1‑68 (MQ=255)
       tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg                                               >  1:1608403/1‑68 (MQ=255)
       tATTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTg                                               >  1:1358238/1‑68 (MQ=255)
        aTTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc                                             <  1:1020402/69‑1 (MQ=255)
        aTTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc                                             <  1:2377084/69‑1 (MQ=255)
        aTTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc                                             <  1:3282052/69‑1 (MQ=255)
        aTTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc                                             <  1:2802177/69‑1 (MQ=255)
        aTTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc                                             <  1:2913467/69‑1 (MQ=255)
        aTTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc                                             <  1:1189094/69‑1 (MQ=255)
        aTTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc                                             <  1:3424297/69‑1 (MQ=255)
        aTTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc                                             <  1:3434855/69‑1 (MQ=255)
        aTTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc                                             <  1:1513831/69‑1 (MQ=255)
        aTTCGGGTCAAACGGCTTGTTGAGCATGTCTGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc                                             <  1:1597225/69‑1 (MQ=255)
        aTTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTc                                             >  1:1262362/1‑69 (MQ=255)
        aTTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTc                                             >  1:1837535/1‑69 (MQ=255)
        aTTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTc                                             >  1:367988/1‑69 (MQ=255)
                      gCTTGTTGATCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGatca                               <  1:2173769/69‑1 (MQ=255)
                      gCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGatca                               <  1:2281200/69‑1 (MQ=255)
                      gCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGatca                               <  1:2982322/69‑1 (MQ=255)
                      gCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGatca                               <  1:1266996/69‑1 (MQ=255)
                      gCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGatca                               <  1:1436369/69‑1 (MQ=255)
                      gCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGatca                               <  1:1591955/69‑1 (MQ=255)
                      gCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGatca                               <  1:3218071/69‑1 (MQ=255)
                      gCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGatca                               <  1:1932738/69‑1 (MQ=255)
                      gCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGatca                               <  1:2026666/69‑1 (MQ=255)
                      gCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGatca                               <  1:339074/69‑1 (MQ=255)
                      gCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGatca                               <  1:3290209/69‑1 (MQ=255)
                      gCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGatca                               <  1:2197324/69‑1 (MQ=255)
                            tgAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATccc                           <  1:3233066/67‑1 (MQ=255)
                            tgAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATccc                           <  1:669537/67‑1 (MQ=255)
                            tgAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATccc                           <  1:8284/67‑1 (MQ=255)
                            tgAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATccc                           <  1:1047339/67‑1 (MQ=255)
                            tgAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATccc                           <  1:2955122/67‑1 (MQ=255)
                            tgAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATccc                           <  1:2054376/67‑1 (MQ=255)
                                cATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCcttctt                      <  1:2031952/68‑1 (MQ=255)
                                 aTGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGGCTTGTCGATCTTCTGGATCATCCCTTCTTTa                    <  1:3367027/69‑1 (MQ=255)
                                 aTGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTa                    <  1:3032035/69‑1 (MQ=255)
                                 aTGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTa                    <  1:965165/69‑1 (MQ=255)
                                 aTGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTa                    <  1:1304521/69‑1 (MQ=255)
                                 aTGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTa                    <  1:1774301/69‑1 (MQ=255)
                                 aTGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTa                    <  1:3062952/69‑1 (MQ=255)
                                 aTGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTa                    <  1:2665351/69‑1 (MQ=255)
                                      tGGATCGAGGTTGCTGAAATTGCTTAACTTCGACTTGTc                                             <  1:681038/39‑1 (MQ=25)
                                              gaTTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCaa       <  1:1700187/69‑1 (MQ=255)
                                              gaTTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCaa       <  1:1153701/69‑1 (MQ=255)
                                              gaTTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCaa       <  1:3140345/69‑1 (MQ=255)
                                              gaTTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCaa       <  1:1353980/69‑1 (MQ=255)
                                              gaTTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCaa       <  1:1491151/69‑1 (MQ=255)
                                              gaTTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCaa       <  1:3259014/69‑1 (MQ=255)
                                              gaTTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCaa       <  1:2911851/69‑1 (MQ=255)
                                              gaTTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCaa       <  1:342518/69‑1 (MQ=255)
                                              gaTTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCaa       <  1:1808225/69‑1 (MQ=255)
                                              gaTTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCaa       <  1:3419579/69‑1 (MQ=255)
                                              gaTTGCGGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCaa       <  1:1801773/69‑1 (MQ=255)
                                                 tGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCAACGt    <  1:814886/69‑1 (MQ=255)
                                                 tGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCAACGt    <  1:2794337/69‑1 (MQ=255)
                                                 tGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCAACGt    <  1:1284535/69‑1 (MQ=255)
                                                 tGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCAACGt    <  1:2486991/69‑1 (MQ=255)
                                                 tGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCAACGt    <  1:254848/69‑1 (MQ=255)
                                                 tGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTATTTACGCATTTTATCAACGt    <  1:817840/69‑1 (MQ=255)
                                                    tGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCTACGTaa  <  1:1580531/68‑1 (MQ=255)
                                                    tGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCAACGTaa  <  1:1462758/68‑1 (MQ=255)
                                                    tGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCAACGTaa  <  1:2703173/68‑1 (MQ=255)
                                                     gAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCAACGt    <  1:364204/65‑1 (MQ=255)
                                                            |                                                           
TAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCAACGTAA  >  minE/761711‑761830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A