Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
876,010 |
A→T |
22.1% |
Q409L (CAG→CTG) |
acnA → |
aconitate hydratase 1 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 876,010 | 0 | A | T | 22.1%
| 147.2
/ 37.5
| 86 | Q409L (CAG→CTG) | acnA | aconitate hydratase 1 |
| Reads supporting (aligned to +/- strand): ref base A (20/47); new base T (3/16); total (23/63) |
| Fisher's exact test for biased strand distribution p-value = 2.58e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01 |
CCCGATGTACCAAAAGCATTTGCCGCCAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCATTGC > minE/875943‑876073
|
cccGATGTACCAAAAGCATTTGCCGCCAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAg > 1:3054232/1‑69 (MQ=255)
cccGATGTACCAAAAGCATTTGCCGCCAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAg > 1:1087011/1‑69 (MQ=255)
cccGATGTACCAAAAGCATTTGCCGCCAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAg > 1:799154/1‑69 (MQ=255)
cccGATGTACCAAAAGCATTTGCCGCCAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAg > 1:2971068/1‑69 (MQ=255)
cccGATGTACCAAAAGCATTTGCCGCCAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAg > 1:1353630/1‑69 (MQ=255)
cccGATGTACCAAAAGCATTTGCCGCCAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAg > 1:167936/1‑69 (MQ=255)
cccGATGTACCAAAAGCATTTGCCGCCAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAg > 1:2847854/1‑69 (MQ=255)
accGATGTACCAAAAGCATTTGCCGCCAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCa > 1:1114150/2‑68 (MQ=255)
ccAAAAGCATTTGCCGCCAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGAt < 1:528006/69‑1 (MQ=255)
ccAAAAGCATTTGCCGCCAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGAt < 1:2366926/69‑1 (MQ=255)
ccAAAAGCATTTGCCGCCAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGAt < 1:3249151/69‑1 (MQ=255)
aGCATTTGCCGCCAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGAttatgt < 1:3199148/69‑1 (MQ=255)
tttGCTGCCAGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGAttatgttatg > 1:2351196/1‑69 (MQ=255)
tttGCTGCCAGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGAttatgttatg > 1:2997486/1‑69 (MQ=255)
tttGCTGCCAGTAGAGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGAttatgttat > 1:1767336/1‑68 (MQ=255)
tGCCGCCAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGAt < 1:675746/58‑1 (MQ=255)
gccAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGa < 1:2466605/69‑1 (MQ=255)
gccAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGa < 1:2557311/69‑1 (MQ=255)
gccAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGa < 1:2065580/69‑1 (MQ=255)
gccAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGa < 1:2059948/69‑1 (MQ=255)
gccAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGa < 1:2391097/69‑1 (MQ=255)
gccAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGa < 1:1606530/69‑1 (MQ=255)
gccAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGa < 1:1650062/69‑1 (MQ=255)
gccAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGa < 1:1607026/69‑1 (MQ=255)
aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATGAACGGAc < 1:3110712/67‑1 (MQ=255)
aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATGAACGGAc < 1:535533/67‑1 (MQ=255)
aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATGAACGGAc < 1:1433024/67‑1 (MQ=255)
aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATGAACGGAc < 1:3430555/67‑1 (MQ=255)
aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATGAACGGAc < 1:262801/67‑1 (MQ=255)
aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATGAACGGAc < 1:2632515/67‑1 (MQ=255)
aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATGAACGGAc < 1:2720807/67‑1 (MQ=255)
aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATGAACGGAc < 1:2091115/67‑1 (MQ=255)
aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATGAACGGAc < 1:2850672/67‑1 (MQ=255)
aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATGAACGGAc < 1:3266315/67‑1 (MQ=255)
aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATGAACGGAc < 1:2867991/67‑1 (MQ=255)
aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATGAACGGAc < 1:2936390/67‑1 (MQ=255)
aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATGAACGGAc < 1:1684701/67‑1 (MQ=255)
aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATGAACGGAc < 1:3010666/67‑1 (MQ=255)
aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATGAACGGAc < 1:3079110/67‑1 (MQ=255)
aGTAGCGAACTGGAAGTGAATGCCACGCATAAAGATCGCCTGCCGGTCGATTATGTTATAAACGGAc < 1:1724625/67‑1 (MQ=255)
aGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGAt < 1:895598/51‑1 (MQ=255)
gAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACatcag < 1:260082/60‑1 (MQ=255)
gAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACatcag < 1:1298866/60‑1 (MQ=255)
gTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCt < 1:1127869/69‑1 (MQ=255)
gTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCt < 1:1865874/69‑1 (MQ=255)
gTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCt < 1:1825309/69‑1 (MQ=255)
gTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCt < 1:3428280/69‑1 (MQ=255)
gTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCt < 1:208161/69‑1 (MQ=255)
gTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCt < 1:448507/69‑1 (MQ=255)
gTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCt < 1:208959/69‑1 (MQ=255)
gTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCt < 1:2485623/69‑1 (MQ=255)
gTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCt < 1:2576361/69‑1 (MQ=255)
tAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGt < 1:2689826/69‑1 (MQ=255)
tAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGt < 1:3035489/69‑1 (MQ=255)
tAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGt < 1:154510/69‑1 (MQ=255)
tAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGt < 1:2630418/69‑1 (MQ=255)
tAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGt < 1:2827749/69‑1 (MQ=255)
tAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGt < 1:2731012/69‑1 (MQ=255)
aaaGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGt < 1:1959495/68‑1 (MQ=255)
aaGATCGCCAGCCGGTCGATTATGTTATGCACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCa > 1:200939/1‑69 (MQ=255)
aaGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTAcc > 1:1228460/1‑52 (MQ=255)
aaGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTAc > 1:1396122/1‑51 (MQ=255)
aaGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATg > 1:2380525/1‑57 (MQ=255)
aaGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTc > 1:2091427/1‑68 (MQ=255)
aaGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTc > 1:869153/1‑68 (MQ=255)
aaGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCa > 1:745315/1‑69 (MQ=255)
aaGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCa > 1:1881471/1‑69 (MQ=255)
aaGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCa > 1:2137679/1‑69 (MQ=255)
aaGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCa > 1:2700016/1‑69 (MQ=255)
aaGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCa > 1:1964987/1‑69 (MQ=255)
aaGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCa > 1:1863553/1‑69 (MQ=255)
aaGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCa > 1:2849429/1‑69 (MQ=255)
gATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCAtt < 1:2994639/69‑1 (MQ=255)
gATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCAtt < 1:593627/69‑1 (MQ=255)
aTCGCCAGCCGGTCGATTATGTTATGAACTGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCAttc < 1:3294051/69‑2 (MQ=255)
aTCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCAtt < 1:492343/68‑1 (MQ=255)
aTCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCAtt < 1:2319748/68‑1 (MQ=255)
aTCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCAtt < 1:1660664/68‑1 (MQ=255)
tCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCATtgc < 1:2041881/69‑1 (MQ=255)
tCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCATtgc < 1:3355249/69‑1 (MQ=255)
tCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCATtgc < 1:594534/69‑1 (MQ=255)
tCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCATtgc < 1:2637107/69‑1 (MQ=255)
tCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCATtgc < 1:2125141/69‑1 (MQ=255)
tCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCATtgc < 1:1682767/69‑1 (MQ=255)
tCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCATtgc < 1:2194506/69‑1 (MQ=255)
tCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCATtgc < 1:1860333/69‑1 (MQ=255)
tCGCCAGCCGGTCGATTATGTTATGAAAGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCATtgc < 1:151777/69‑1 (MQ=255)
|
CCCGATGTACCAAAAGCATTTGCCGCCAGTAACGAACTGGAAGTGAATGCCACGCATAAAGATCGCCAGCCGGTCGATTATGTTATGAACGGACATCAGTATCAGTTACCTGATGGCGCTGTGGTCATTGC > minE/875943‑876073
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
