Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,106,450 A→G 31.7% H81R (CAC→CGC)  agaA → predicted truncated N‑acetylgalactosamine‑6‑phosphate deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,106,4500AG31.7% 116.7 / 69.8 132H81R (CAC→CGC) agaApredicted truncated N‑acetylgalactosamine‑6‑phosphate deacetylase
Reads supporting (aligned to +/- strand):  ref base A (29/61);  new base G (40/2);  total (69/63)
Fisher's exact test for biased strand distribution p-value = 1.04e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.44e-01

Only 100 of 133 total aligned reads displayed.

GACGCGATGCAGGCAGCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCACGGTGGCGTTGTCCGTACCGCGTCTGGTGGGCTGGCGGGCAGTACGCTGTCTGTTGATGCGGCAGT  >  minE/2106383‑2106516
                                                                   |                                                                  
gACGCGATGCAGGCAGCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCa                                                                    >  1:729008/1‑68 (MQ=255)
gACGCGATGCAGGCAGCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCAc                                                                   >  1:174216/1‑69 (MQ=255)
gACGCGATGCAGGCAGCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCAc                                                                   >  1:2624768/1‑69 (MQ=255)
gACGCGATGCAGGCAGCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCAc                                                                   >  1:2293699/1‑69 (MQ=255)
gACGCGATGCAGGCAGCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCAc                                                                   <  1:2257034/69‑1 (MQ=255)
gACGCGATGCAGGCAGCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCAc                                                                   <  1:2224543/69‑1 (MQ=255)
gACGCGATGCAGGCAGCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCAc                                                                   >  1:2139870/1‑69 (MQ=255)
gACGCGATGCAGGCAGCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCAc                                                                   >  1:2944434/1‑69 (MQ=255)
gACGCGATGCAGGCAGCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCAc                                                                   >  1:2966921/1‑69 (MQ=255)
gACGCGATGCAGGCAGCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCAc                                                                   >  1:1576214/1‑69 (MQ=255)
gACGCGATGCAGGCAGCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCAc                                                                   >  1:3205748/1‑69 (MQ=255)
gACGCGATGCAGGCAGCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCAc                                                                   <  1:1360820/69‑1 (MQ=255)
gACGCGATGCAGGCAGCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCAc                                                                   <  1:1338802/69‑1 (MQ=255)
gACGCGATGCAGGCAGCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCAc                                                                   <  1:750466/69‑1 (MQ=255)
gACGCGATGCAGGCAGCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCAc                                                                   >  1:961284/1‑69 (MQ=255)
gACGCGATGCAGGCAGCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCAc                                                                   >  1:94756/1‑69 (MQ=255)
gACGCGATGCAGGCAGCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCAc                                                                   >  1:107911/1‑69 (MQ=255)
gACGCGATGCAGGCAGCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCAc                                                                   <  1:918360/69‑1 (MQ=255)
gACGCGATGCAGGCAGCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCAc                                                                   <  1:591080/69‑1 (MQ=255)
gACGCGATGCAGGCAGCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCAc                                                                   <  1:1268146/69‑1 (MQ=255)
  cgcgATGCAGGCAGCCGGGATGCCGGATGGTCACTATACGTTATGTGGTGAAGAAGTGCAGATGCGCgg                                                                 >  1:2421211/1‑69 (MQ=255)
  cgcgATGCAGGCAGCCGGGATGCCGGATGGTCACTATACGTTATGTGGTGAAGAAGTGCAGATGCGCgg                                                                 >  1:1718388/1‑69 (MQ=255)
  cgcgATGCAGGCAGCCGGGATGCCGGATGGTCACTATACGTTATGTGGTGAAGAAGTGCAGATGCGCgg                                                                 >  1:2342623/1‑69 (MQ=255)
  cgcgATGCAGGCAGCCGGGATGCCGGATGGTCACTATACGTTATGTGGTGAAGAAGTGCAGATGCGCgg                                                                 >  1:293025/1‑69 (MQ=255)
  cgcgATGCAGGCAGCCGGGATGCCGGATGGTCACTATACGTTATGTGGTGAAGAAGTGCAGATGCGCgg                                                                 >  1:1796133/1‑69 (MQ=255)
  cgcgATGCAGGCAGCCGGGATGCCGGATGGTCACTATACGTTATGTGGTGAAGAAGTGCAGATGCGCgg                                                                 >  1:3317427/1‑69 (MQ=255)
  cgcgATGCAGGCAGCCGGGATGCCGGATGGTCACTATACGTTATGTGGTGAAGAAGTGCAGATGCGCgg                                                                 >  1:3157050/1‑69 (MQ=255)
  cgcgATGCAGGCAGCCGGGATGCCGGATGGTCACTATACGTTATGTGGTGAAGAAGTGCAGATGCGCgg                                                                 >  1:906331/1‑69 (MQ=255)
  cgcgATGCAGGCAGCCGGGATGCCGGATGGTCACTATACGTTATGTGGTGAAGAAGTGCAGATGCGCgg                                                                 >  1:2028636/1‑69 (MQ=255)
      aTGCAGGCAGCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCAc                                                                   <  1:693553/63‑1 (MQ=255)
       tGCAGGCAGCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCACGGTGGc                                                             <  1:2933109/68‑1 (MQ=255)
         caggcagCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCACGGTGGCGtt                                                          >  1:1495420/1‑69 (MQ=255)
           ggcagCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCACGGTGGCGTTg                                                         <  1:375653/68‑1 (MQ=255)
           ggcagCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCACGGTGGCGTTg                                                         <  1:1303999/68‑1 (MQ=255)
           ggcagCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCACGGTGGCGTTg                                                         <  1:2660517/68‑1 (MQ=255)
           ggcagCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCACGGTGGCGTTg                                                         <  1:2376254/68‑1 (MQ=255)
           ggcagCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCACGGTGGCGTTg                                                         <  1:2833744/68‑1 (MQ=255)
           ggcagCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCACGGTGGCGTTg                                                         <  1:414179/68‑1 (MQ=255)
           ggcagCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCACGGTGGCGTTg                                                         <  1:1159718/68‑1 (MQ=255)
           ggcagCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCACGGTGGCGTTg                                                         <  1:3186719/68‑1 (MQ=255)
           ggcagCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCACGGTGGCGTTg                                                         <  1:2959457/68‑1 (MQ=255)
           ggcagCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCACGGTGGCGTTGt                                                        >  1:3201606/1‑69 (MQ=255)
                        ccGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCACGGTGGCGTTGTCCGTACCGCGTCt                                           <  1:459059/69‑1 (MQ=255)
                        ccGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCACGGTGGCGTTGTCCGTACCGCGTCt                                           <  1:642093/69‑1 (MQ=255)
                        ccGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCACGGTGGCGTTGTCCGTACCGCGTCt                                           <  1:2396471/69‑1 (MQ=255)
                        ccGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCACGGTGGCGTTGTCCGTACCGCGTCt                                           <  1:2768259/69‑1 (MQ=255)
                         cGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCACGGTGGCGTTGTCCGTACCGCGTCt                                           <  1:3139359/68‑1 (MQ=255)
                               gTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCACGGTGGCGTTGTCCg                                                     <  1:971336/52‑1 (MQ=255)
                               gTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCACGGTGGCGTTGTCCg                                                     <  1:2235663/52‑1 (MQ=255)
                               gTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCACGGTGGCGTTGTCCg                                                     <  1:2139342/52‑1 (MQ=255)
                                 cacTATACGTTATGTGGTGAAGAAGTGCAGATGCGCGGTGGCGTTGTCCGTACCGCGTCCGGTGGGCTg                                  >  1:1395694/3‑69 (MQ=255)
                                 cacTATACGTTATGTGGTGAAGAAGTGCAGATGCGCGGTGGCGTTGTCCGTACCGCGTCCGGTGGGCTg                                  >  1:2584278/3‑69 (MQ=255)
                                 cacTATACGTTATGTGGTGAAGAAGTGCAGATGCGCGGTGGCGTTGTCCGTACCGCGTCCGGTGGGCTg                                  >  1:502823/3‑69 (MQ=255)
                                 cacTATACGTTATGTGGTGAAGAAGTGCAGATGCGCGGTGGCGTTGTCCGTACCGCGTCCGGTGGGCTg                                  >  1:2398403/3‑69 (MQ=255)
                                 cacTATACGTTATGTGGTGAAGAAGTGCAGATGCGCGGTGGCGTTGTCCGTACCGCGTCCGGTGGGCTg                                  >  1:2882927/3‑69 (MQ=255)
                                 cacTATACGTTATGTGGTGAAGAAGTGCAGATGCGCGGTGGCGTTGTCCGTACCGCGTCCGGTGGGCTg                                  <  1:1891461/67‑1 (MQ=255)
                                 cacTATACGTTATGTGGTGAAGAAGTGCAGATGCGCGGTGGCGTTGTCCGTACCGCGTCCGGTGGGCTg                                  >  1:588037/3‑69 (MQ=255)
                                 cacTATACGTTATGTGGTGAAGAAGTGCAGATGCGCGGTGGCGTTGTCCGTACCGCGTCCGGTGGGCTg                                  >  1:3088756/3‑69 (MQ=255)
                                        cGTTATGTGGTGAAGAAGTGCAGATGCACGGTGGCGTTGt                                                        >  1:3363727/1‑40 (MQ=255)
                                        cGTTATGTGGTGAAGAAGTGCAGATGCACGGTGGCGTTGTCCGTACCGCGTCTGGTGGGCTGGCGGGc                            >  1:1178017/1‑68 (MQ=255)
                                          ttATGTGGTGAAGAAGTGCAGATGCACGGTGGCGTTGTCCGTACCGCGTCTGGTGGGCTGGCGGGCAg                          <  1:1239507/68‑1 (MQ=255)
                                          ttATGTGGTGAAGAAGTGCAGATGCACGGTGGCGTTGTCCGTACCGCGTCTGGTGGGCTGGCGGGCAg                          <  1:3415784/68‑1 (MQ=255)
                                          ttATGTGGTGAAGAAGTGCAGATGCACGGTGGCGTTGTCCGTACCGCGTCTGGTGGGCTGGCGGGCAg                          <  1:1508052/68‑1 (MQ=255)
                                          ttATGTGGTGAAGAAGTGCAGATGCACGGTGGCGTTGTCCGTACCGCGTCTGGTGGGCTGGCGGGCAg                          <  1:1891084/68‑1 (MQ=255)
                                          ttATGTGGTGAAGAAGTGCAGATGCACGGTGGCGTTGTCCGTACCGCGTCTGGTGGGCTGGCGGGCAg                          <  1:704249/68‑1 (MQ=255)
                                          ttATGTGGTGAAGAAGTGCAGATGCACGGTGGCGTTGTCCGTACCGCGTCTGGTGGGCTGGCGGGCAg                          <  1:875917/68‑1 (MQ=255)
                                          ttATGTGGTGAAGAAGTGCAGATGCACGGTGGCGTTGTCCGTACCGCGTCTGGTGGGCTGGCGGGCAg                          <  1:2700222/68‑1 (MQ=255)
                                          ttATGTGGTGAAGAAGTGCAGATGCACGGTGGCGTTGTCCGTACCGCGTCTGGTGGGCTGGCGGGCAg                          <  1:2655887/68‑1 (MQ=255)
                                                 gtgAAGAAGTGCAGATGCGCGGTGGCGTTGTCCGTACcgc                                               >  1:3280119/1‑40 (MQ=255)
                                                 gtgAAGAAGTGCAGATGCGCGGTGGCGTTGTCCGTACCGCGTCCGGTGGGCTGGCGGGCAGTACGctgt                  >  1:1013192/1‑69 (MQ=255)
                                                 gtgAAGAAGTGCAGATGCGCGGTGGCGTTGTCCGTACCGCGTCCGGTGGGCTGGCGGGCAGTACGctgt                  >  1:695963/1‑69 (MQ=255)
                                                 gtgAAGAAGTGCAGATGCGCGGTGGCGTTGTCCGTACCGCGTCCGGTGGGCTGGCGGGCAGTACGctgt                  >  1:658384/1‑69 (MQ=255)
                                                 gtgAAGAAGTGCAGATGCGCGGTGGCGTTGTCCGTACCGCGTCCGGTGGGCTGGCGGGCAGTACGctgt                  >  1:1299965/1‑69 (MQ=255)
                                                 gtgAAGAAGTGCAGATGCGCGGTGGCGTTGTCCGTACCGCGTCCGGTGGGCTGGCGGGCAGTACGctgt                  >  1:847607/1‑69 (MQ=255)
                                                 gtgAAGAAGTGCAGATGCGCGGTGGCGTTGTCCGTACCGCGTCCGGTGGGCTGGCGGGCAGTACGctgt                  >  1:1126377/1‑69 (MQ=255)
                                                 gtgAAGAAGTGCAGATGCGCGGTGGCGTTGTCCGTACCGCGTCCGGTGGGCTGGCGGGCAGTACGctgt                  >  1:1073495/1‑69 (MQ=255)
                                                 gtgAAGAAGTGCAGATGCGCGGTGGCGTTGTCCGTACCGCGTCCGGTGGGCTGGCGGGCAGTACGctgt                  >  1:1413609/1‑69 (MQ=255)
                                                 gtgAAGAAGTGCAGATGCGCGGTGGCGTTGTCCGTACCGCGTCCGGTGGGCTGGCGGGCAGTACGctgt                  >  1:3421946/1‑69 (MQ=255)
                                                 gtgAAGAAGTGCAGATGCGCGGTGGCGTTGTCCGTACCGCGTCCGGTGGGCTGGCGGGCAGTACGctgt                  >  1:3004332/1‑69 (MQ=255)
                                                 gtgAAGAAGTGCAGATGCGCGGTGGCGTTGTCCGTACCGCGTCCGGTGGGCTGGCGGGCAGTACGctg                   >  1:658275/1‑68 (MQ=255)
                                                 gtgAAGAAGTGCAGATGCACGGTGGCGTTGTCCGTACCGCGTCTGGTGGGCTGGCGGGCAGTACGctgt                  >  1:1286240/1‑69 (MQ=255)
                                                      gaagTGCAGATGCACGGTGGCGTTGTCCGTACCGCGTCTGGTGGGCTGGCGGGCAGTACGCTGTCTGtt             <  1:1430432/69‑1 (MQ=255)
                                                      gaagTGCAGATGCACGGTGGCGTTGTCCGTACCGCGTCTGGTGGGCTGGCGGGCAGTACGCTGTCTGtt             <  1:2060124/69‑1 (MQ=255)
                                                      gaagTGCAGATGCACGGTGGCGTTGTCCGTACCGCGTCTGGTGGGCTGGCGGGCAGTACGCTGTCTGtt             <  1:838267/69‑1 (MQ=255)
                                                      gaagTGCAGATGCACGGTGGCGTTGTCCGTACCGCGTCTGGTGGGCTGGCGGGCAGTACGCTGTCTGtt             <  1:3041637/69‑1 (MQ=255)
                                                      gaagTGCAGATGCACGGTGGCGTTGTCCGTACCGCGTCTGGTGGGCTGGCGGGCAGTACGCTGTCTGtt             <  1:60221/69‑1 (MQ=255)
                                                       aagTGCAGATGCACGGTGGCGTTGTCCGTACCGCGTCTGGTGGGCTTGCGGGCAGTACGCTGTCTGtt             <  1:2099330/68‑1 (MQ=255)
                                                                tGCACGGTGGCGTTGTCCGTACCGCGTCTGGTGGGCTGGCGGGCAGTACTCTGTCTGTTGATGCGGCAg   <  1:1818957/69‑1 (MQ=255)
                                                                tGCACGGTGGCGTTGTCCGTACCGCGTCTGGTGGGCTGGCGGGCAGTACGCTGTCTGTTGATGCGGCAg   <  1:1865717/69‑1 (MQ=255)
                                                                tGCACGGTGGCGTTGTCCGTACCGCGTCTGGTGGGCTGGCGGGCAGTACGCTGTCTGTTGATGCGGCAg   <  1:1334998/69‑1 (MQ=255)
                                                                tGCACGGTGGCGTTGTCCGTACAGCGTCTGGTGGGCTGGCGGGCAGTACGCTGTCTGTTGATGCGGCAg   <  1:2971168/69‑1 (MQ=255)
                                                                 gCACGGTGGCGTTGTCCGTACCGCGTCTGGTGGGCTGGCGGGCAGTACGCTGTCTGtt             >  1:1017850/1‑58 (MQ=255)
                                                                 gCACGGTGGCGTTGTCCGTACCGCGTCTGGTGGGCTGGCGGGCAGTACGCTGTCTGTTGATGCGGCAg   >  1:2008087/1‑68 (MQ=255)
                                                                 gCACGGTGGCGTTGTCCGTACCGCGTCTGGTGGGCTGGCGGGCAGTACGCTGTCTGTTGATGCGGCAg   <  1:1502925/68‑1 (MQ=255)
                                                                 gCACGGTGGCGTTGTCCGTACCGCGTCTGGTGGGCTGGCGGGCAGTACGCTGTCTGTTGATGCGGCAGt  >  1:3101622/1‑69 (MQ=255)
                                                                 gCACGGTGGCGTTGTCCGTACCGCGTCTGGTGGGCTGGCGGGCAGTACGCTGTCTGTTGATGCGGCAGt  >  1:177559/1‑69 (MQ=255)
                                                                 gCACGGTGGCGTTGTCCGTACCGCGTCTGGTGGGCTGGCGGGCAGTACGCTGTCTGTTGATGCGGCAGt  >  1:743809/1‑69 (MQ=255)
                                                                 gCACGGTGGCGTTGTCCGTACCGCGTCTGGTGGGCTGGCGGGCAGTACGCTGTCTGTTGATGCGGCAGt  >  1:121402/1‑69 (MQ=255)
                                                                 gCACGGTGGCGTTGTCCGTACCGCGTCTGGTGGGCTGGCGGGCAGTACGCTGTCTGTTGATGCGGCAGt  >  1:792173/1‑69 (MQ=255)
                                                                 gCACGGTGGCGTTGTCCGTACCGCGTCTGGTGGGCTGGCGGGCAGTACGCTGTCTGTTGATGCGGCAGt  >  1:2612538/1‑69 (MQ=255)
                                                                   |                                                                  
GACGCGATGCAGGCAGCTGGGATGCCGGATGGTCGCTATACGTTATGTGGTGAAGAAGTGCAGATGCACGGTGGCGTTGTCCGTACCGCGTCTGGTGGGCTGGCGGGCAGTACGCTGTCTGTTGATGCGGCAGT  >  minE/2106383‑2106516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A