Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I3 R1	16	56.1	1756558	98.6%	1731966	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,178,128	T→G	L5R (CTC→CGC)	tolC →	transport channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,178,128	0	T	G	100.0%	193.5 / NA	52	L5R (CTC→CGC)	tolC	transport channel
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (27/25); total (27/25)

CATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCTCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAA  >  NC_000913/3177981‑3178270

cATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGccc                                                                                                                                             >  2:149955/1‑151 (MQ=255)

       tAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCt                                                                                                                                       <  1:563923/150‑1 (MQ=255)

        aaaTTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCtt                                                                                                                                      >  2:18035/1‑150 (MQ=255)

         aaTTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTAt                                                                                                                                    <  1:289734/151‑1 (MQ=255)

            tGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCg                                                                                                                                  <  1:427009/150‑1 (MQ=255)

              aGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGcc                                                                                                                               <  2:794260/151‑1 (MQ=255)

                gCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTg                                                                                                                             <  1:626451/151‑1 (MQ=255)

                 cacaTTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGa                                                                                                                            <  2:705379/151‑1 (MQ=255)

                 cacaTTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGa                                                                                                                            <  2:301063/151‑1 (MQ=255)

                  acaTTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAg                                                                                                                           <  1:352881/151‑1 (MQ=255)

                   caTTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGc                                                                                                                          >  2:673229/1‑151 (MQ=255)

                     ttAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCt                                                                                                                        >  1:548988/1‑151 (MQ=255)

                         cGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCt                                                                                                                    <  1:49340/151‑1 (MQ=255)

                           cccTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTgg                                                                                                                  >  1:502347/1‑151 (MQ=255)

                                tGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGtt                                                                                                               >  1:216390/1‑149 (MQ=255)

                                  gCACGTAACGCCAACCTTTTG‑GGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGtt                                                                                                          >  2:532650/1‑151 (MQ=255)

                                    aCGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTc                                                                                                         <  2:279066/151‑1 (MQ=255)

                                        aaCGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGtt                                                                                                      <  2:534126/150‑1 (MQ=255)

                                         aCGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGa                                                                                                    <  1:109942/151‑1 (MQ=255)

                                                      gCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCgaga                                                                                        <  1:765921/150‑1 (MQ=255)

                                                      gCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCgag                                                                                         >  2:805832/1‑149 (MQ=255)

                                                        ggTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAAcc                                                                                     >  1:34439/1‑151 (MQ=255)

                                                         gTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCt                                                                                    <  1:160110/151‑1 (MQ=255)

                                                           aGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTg                                                                                   >  2:364246/1‑150 (MQ=255)

                                                              ggCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGc                                                                               >  2:421912/1‑151 (MQ=255)

                                                                      cTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTa                                                                        >  2:197520/1‑150 (MQ=255)

                                                                         gAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTa                                                                        >  2:310516/1‑147 (MQ=255)

                                                                                cAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCAc                                                             <  2:48924/151‑1 (MQ=255)

                                                                                    aatTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGcc                                                          <  2:727764/150‑1 (MQ=255)

                                                                                       tttACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCtt                                                        >  1:599455/1‑149 (MQ=255)

                                                                                         tACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAg                                                      <  2:578357/149‑1 (MQ=255)

                                                                                         tACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGt                                                     >  2:372452/1‑150 (MQ=255)

                                                                                          aCAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTa                                                    <  1:177947/150‑1 (MQ=255)

                                                                                             gTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAAccc                                                >  2:753329/1‑151 (MQ=255)

                                                                                             gTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAAccc                                                >  1:838584/1‑151 (MQ=255)

                                                                                              tttGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCg                                               >  2:650208/1‑151 (MQ=255)

                                                                                                    cgcgCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTg                                         <  2:800317/151‑1 (MQ=255)

                                                                                                        cTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGt                                      >  1:753410/1‑150 (MQ=255)

                                                                                                           aaTACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGt                                  >  1:343287/1‑151 (MQ=255)

                                                                                                                 gCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTgcc                             <  2:746892/150‑1 (MQ=255)

                                                                                                                   ttCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTgccgcc                          >  1:162426/1‑151 (MQ=255)

                                                                                                                     caccacAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGccgccg                         >  1:127511/1‑150 (MQ=255)

                                                                                                                            aGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGAt                 >  2:228303/1‑151 (MQ=255)

                                                                                                                                     aaTGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCttt        <  2:281431/151‑1 (MQ=255)

                                                                                                                                     aaTGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAATTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTGGCAGCAAGCACGCCTTAGTAGCCCGGAATTGCGGAAGTCTGCCGCCGATCGTGATGCTGCCttt        <  1:649854/151‑1 (MQ=255)

                                                                                                                                        gaagaaATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGt                                      <  1:862203/118‑1 (MQ=255)

                                                                                                                                        gaagaaATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGt                                      >  2:862302/1‑118 (MQ=255)

                                                                                                                                          agaaATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCTGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGaaaa   <  1:685490/151‑1 (MQ=255)

                                                                                                                                          agaaATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGaaaa   <  2:37712/151‑1 (MQ=255)

                                                                                                                                          agaaATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGaaa    >  2:491147/1‑150 (MQ=255)

                                                                                                                                           gaaATTGCGCGCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTAGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTCGTAACCCGGACTTGCGAAAGTCTGCCGCCGATCGTGATGCTGCCTTTGaaaa   >  1:353661/1‑150 (MQ=255)

                                                                                                                                            aaaTTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGaaaaa  >  2:353911/1‑150 (MQ=255)

CATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCTCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAA  >  NC_000913/3177981‑3178270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A