Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I1 R1	16	44.5	1394873	98.4%	1372555	150.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,178,128	T→G	L5R (CTC→CGC)	tolC →	transport channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,178,128	0	T	G	100.0%	194.0 / NA	53	L5R (CTC→CGC)	tolC	transport channel
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (27/26); total (27/26)

ATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCTCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTA  >  NC_000913/3177982‑3178274

aTGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGcccc                                                                                                                                                <  2:50732/151‑1 (MQ=255)

  gtgtTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCa                                                                                                                                               <  2:92094/150‑1 (MQ=255)

         aTTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATc                                                                                                                                       <  2:358286/151‑1 (MQ=255)

           tGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCgg                                                                                                                                     >  2:95680/1‑151 (MQ=255)

                cacaTTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGa                                                                                                                                <  2:557024/151‑1 (MQ=255)

                cacaTTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTAACGGCCTg                                                                                                                                 >  1:191320/1‑150 (MQ=255)

                      aaCGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCttt                                                                                                                          <  2:117625/151‑1 (MQ=255)

                      aaCGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCttt                                                                                                                          <  1:561361/151‑1 (MQ=255)

                           ccTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTggg                                                                                                                     >  1:265527/1‑151 (MQ=255)

                            cTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGTAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGt                                                                                                                    >  1:693437/1‑151 (MQ=255)

                               tGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTc                                                                                                                  >  1:375487/1‑150 (MQ=255)

                                  cACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGtt                                                                                                              >  2:468794/1‑151 (MQ=255)

                                  cACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGtt                                                                                                              >  2:460377/1‑151 (MQ=255)

                                    cGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCg                                                                                                            >  2:484161/1‑151 (MQ=255)

                                       aaCGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTg                                                                                                         <  1:611365/151‑1 (MQ=255)

                                       aaCGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTg                                                                                                         >  2:229832/1‑151 (MQ=255)

                                            cAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCa                                                                                                    <  2:436298/151‑1 (MQ=255)

                                                    tGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCGGGcc                                                                                                <  1:18235/147‑1 (MQ=255)

                                                    tGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCg                                                                                               >  1:388612/1‑148 (MQ=255)

                                                     gCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCgaga                                                                                            <  1:285398/150‑1 (MQ=255)

                                                     gCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGaa                                                                                           <  2:289597/151‑1 (MQ=255)

                                                       ggTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAAcc                                                                                         <  1:232526/151‑1 (MQ=255)

                                                         tAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTg                                                                                       >  2:315879/1‑151 (MQ=255)

                                                              ggtgcTGCTAGAATCCGCAATAATTTGACAGTTTGATCGCGCTAAATACTGCTGCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCTAGGCCGAGAACCTGATg                                                                                    <  1:367812/145‑1 (MQ=255)

                                                               cTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCa                                                                                  >  2:256956/1‑150 (MQ=255)

                                                                  cTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGtt                                                                              >  1:132039/1‑151 (MQ=255)

                                                                    gCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGtt                                                                              <  1:205659/149‑1 (MQ=255)

                                                                     cTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTAt                                                                           >  1:30551/1‑151 (MQ=255)

                                                                       aGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTAt                                                                           <  1:557709/149‑1 (MQ=255)

                                                                          aTCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGACCCTGATGCAAGTTTATCagc                                                                       >  2:328252/1‑150 (MQ=255)

                                                                              gCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCagcaagc                                                                   >  1:443512/1‑150 (MQ=255)

                                                                                 ataatTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGc                                                               >  2:400466/1‑151 (MQ=255)

                                                                                    atTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCtt                                                            >  2:55294/1‑151 (MQ=255)

                                                                                           aGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAAcc                                                     >  1:127274/1‑151 (MQ=255)

                                                                                               tGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCgg                                                  <  2:213006/150‑1 (MQ=255)

                                                                                                  tCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAAtt                                              >  1:346205/1‑151 (MQ=255)

                                                                                                  tCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAAtt                                              >  2:504064/1‑151 (MQ=255)

                                                                                                     cgcTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCg                                           >  2:7123/1‑151 (MQ=255)

                                                                                                         aaaTACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTaa                                        <  1:188591/150‑1 (MQ=255)

                                                                                                         aaaTACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTaa                                        <  2:657079/150‑1 (MQ=255)

                                                                                                         aaaTACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTa                                         >  1:405941/1‑149 (MQ=255)

                                                                                                          aaTACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGt                                      >  2:237053/1‑151 (MQ=255)

                                                                                                             aCTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCt                                    <  2:233113/150‑1 (MQ=255)

                                                                                                             aCTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTg                                   >  2:28679/1‑151 (MQ=255)

                                                                                                                      ccacAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGACCCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGAt                           <  1:374733/150‑1 (MQ=255)

                                                                                                                      ccacAAGGAATGCAAATGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTGCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATc                          <  1:460481/151‑1 (MQ=255)

                                                                                                                          aaGGAATGCAAATGAAGAAATTGCGCCCCATTCTTAACGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGa                      <  2:191341/151‑1 (MQ=255)

                                                                                                                                    aaTGAAGAAATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCttt            >  2:220140/1‑151 (MQ=255)

                                                                                                                                       gaagaaATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGaa         <  2:131965/151‑1 (MQ=255)

                                                                                                                                       gaagaaATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGaa         <  1:522903/151‑1 (MQ=255)

                                                                                                                                       gaagaaATTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGa          <  1:322528/150‑1 (MQ=255)

                                                                                                                                           aaaTTGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGaaaaaa     <  2:465720/151‑1 (MQ=255)

                                                                                                                                               tGCGCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTa  >  1:19065/1‑150 (MQ=255)

ATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCTCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTA  >  NC_000913/3177982‑3178274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A