Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I3 R1 283 58.3 1819865 98.8% 1798026 150.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,313,733 A→G intergenic (‑70/‑287) yciC ← / → ompW UPF0259 family inner membrane protein/outer membrane protein W

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,313,7330AG100.0% 150.0 / NA 42intergenic (‑70/‑287)yciC/ompWUPF0259 family inner membrane protein/outer membrane protein W
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/22);  total (20/22)

ACAGAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCTGGCCTTCAGGCATGGGCAAATCGCGGCAAGTGGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGATTTAAATCACATTAACCAG  >  NC_000913/1313591‑1313880
                                                                                                                                              |                                                                                                                                                   
aCAGAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAgg                                                                                                                                             >  1:322102/1‑151 (MQ=255)
  aGAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTc                                                                                                                                           <  1:700236/151‑1 (MQ=255)
   gAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCt                                                                                                                                          <  2:218114/151‑1 (MQ=255)
       ggTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTa                                                                                                                                      <  1:452554/151‑1 (MQ=255)
           aTAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATgg                                                                                                                                   >  2:686298/1‑150 (MQ=255)
           aTAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGa                                                                                                                                  <  1:224474/151‑1 (MQ=255)
            tAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGa                                                                                                                                  >  2:258777/1‑150 (MQ=255)
             aaaTTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGaa                                                                                                                                 >  2:562561/1‑150 (MQ=255)
              aaTTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGaaa                                                                                                                                >  2:665710/1‑150 (MQ=255)
               aTTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGaaa                                                                                                                                >  2:906555/1‑149 (MQ=255)
                 tGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGAAAAGc                                                                                                                             >  2:721124/1‑150 (MQ=255)
                  gATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGAAAAGCa                                                                                                                            <  1:360126/150‑1 (MQ=255)
                    ttACGGAAGAAATTTCTGGTGTCACGGGATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGAAAAGCatat                                                                                                                         >  2:703858/1‑151 (MQ=255)
                            gaaATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTaa                                                                                                                 <  2:748281/151‑1 (MQ=255)
                            gaaATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTaa                                                                                                                 <  1:293274/151‑1 (MQ=255)
                            gaaATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTa                                                                                                                  >  1:52554/1‑150 (MQ=255)
                                 ttCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCg                                                                                                             <  2:694115/150‑1 (MQ=255)
                                      gtgtCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTa                                                                                                       <  2:150018/151‑1 (MQ=255)
                                         tCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAAtt                                                                                                    >  2:687478/1‑151 (MQ=255)
                                          cACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAAtt                                                                                                    <  1:334368/150‑1 (MQ=255)
                                           aCGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAAtt                                                                                                    <  2:741179/149‑1 (MQ=255)
                                                              gTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCTGGCCTTCAGGCATGGGc                                                                                >  1:779832/1‑150 (MQ=255)
                                                                   aGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCTGGCCTTCAGGCATGGGCAAATc                                                                           <  2:558251/150‑1 (MQ=255)
                                                                    gACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCTGGCCTTCAGGCATGGGCAAATcgc                                                                         <  1:907882/151‑1 (MQ=255)
                                                                           aCTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCTGGCCTTCAGGCATGGGCAAATCGCGGCAAGt                                                                  <  1:148931/151‑1 (MQ=255)
                                                                                        tttATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCTGGCCTTCAGGCATGGGCAAATCGCGGCAAGTGGCGACCGAGtt                                                      >  1:834783/1‑150 (MQ=255)
                                                                                         ttATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCTGGCCTTCAGGCATGGGCAAATCGCGGCAAGTGGCGACCGAGTTa                                                     >  1:204075/1‑150 (MQ=255)
                                                                                              cAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCTGGCCTTCAGGCATGGGCAAATCGCGGCAAGTGGCGACCGAGTTAATAttt                                               <  1:627120/151‑1 (MQ=255)
                                                                                               aGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCTGGCCTTCAGGCATGGGCAAATCGCGGCAAGTGGCGACCGAGTTAATATTTg                                              >  2:757824/1‑151 (MQ=255)
                                                                                                ggTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCTGGCCTTCAGGCATGGGCAAATCGCGGCAAGTGGCGACCGAGTTAATATTTGc                                             <  1:492194/151‑1 (MQ=255)
                                                                                                       aTTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCTGGCCTTCAGGCATGGGCAAATCGCGGCAAGTGGCGACCGAGTTAATATTTGCGTAGCg                                       >  2:899929/1‑150 (MQ=255)
                                                                                                            cGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCTGGCCTTCAGGCATGGGCAAATCGCGGCAAGTGGCGACCGAGTTAATATTTGCGTAGCGAAAata                                 <  1:719740/151‑1 (MQ=255)
                                                                                                             gggCAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCTGGCCTTCAGGCATGGGCAAATCGCGGCAAGTGGCGACCGAGTTAATATTTGCGTAGCGAAAatat                                >  2:303245/1‑151 (MQ=255)
                                                                                                                cAATTGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCTGGCCTTCAGGCATGGGCAAATCGCGGCAAGTGGCGACCGAGTTAATATTTGCGTAGCGAAAATAttt                              >  2:549511/1‑150 (MQ=255)
                                                                                                                    tGTACCCTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCTGGCCTTCAGGCATGGGCAAATCGCGGCAAGTGGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTaaaa                          <  2:50911/150‑1 (MQ=255)
                                                                                                                        cccTGGTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCTGGCCTTCAGGCATGGGCAAATCGCGGCAAGTGGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTg                      >  1:174395/1‑150 (MQ=255)
                                                                                                                            ggTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCTGGCCTTCAGGCATGGGCAAATCGCGGCAAGTGGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGAttt                  <  1:651723/150‑1 (MQ=255)
                                                                                                                            ggTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCTGGCCTTCAGGCATGGGCAAATCGCGGCAAGTGGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGATTTa                 <  1:428944/151‑1 (MQ=255)
                                                                                                                             gTTAAGCCTCGGGTGGCGGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCTGGCCTTCAGGCATGGGCAAATCGCGGCAAGTGGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGATTTa                 >  1:417632/1‑150 (MQ=255)
                                                                                                                                   ccTCGGGTGGCGGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCTGGCCTTCAGGCATGGGCAAATCGCGGCAAGTGGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGATTTAAATcaca          <  1:427825/151‑1 (MQ=255)
                                                                                                                                      cGGGTGGCGGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCTGGCCTTCAGGCATGGGCAAATCGCGGCAAGTGGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGATTTAAATCACATTa       <  1:902137/151‑1 (MQ=255)
                                                                                                                                       gggTGGCGGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCTGGCCTTCAGGCATGGGCAAATCGCGGCAAGTGGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGATTTAAATCACATTa       >  2:471710/1‑150 (MQ=255)
                                                                                                                                           ggcggcATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCTGGCCTTCAGGCATGGGCAAATCGCGGCAAGTGGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGATTTAAATCACATTAACCAg  <  1:731640/151‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
ACAGAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCTGGCCTTCAGGCATGGGCAAATCGCGGCAAGTGGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGATTTAAATCACATTAACCAG  >  NC_000913/1313591‑1313880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A