Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F50 I3 R1
|
283 |
58.3 |
1819865 |
98.8% |
1798026 |
150.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,435,623 |
A→G |
intergenic (‑4/+137) |
uspF ← / ← ompN |
stress‑induced protein, ATP‑binding protein/outer membrane pore protein N, non‑specific |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,435,623 | 0 | A | G | 96.0%
| 165.8
/ NA
| 50 | intergenic (‑4/+137) | uspF/ompN | stress‑induced protein, ATP‑binding protein/outer membrane pore protein N, non‑specific |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (18/30); minor base C (0/2); total (18/32) |
| Fisher's exact test for biased strand distribution p-value = 5.30e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 |
AGGCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGA > NC_000913/1435475‑1435769
|
atgCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGcc < 2:509768/149‑1 (MQ=255)
gtagGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGtt > 1:85572/1‑151 (MQ=255)
agGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGtttt < 1:502855/151‑1 (MQ=255)
cAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGt > 2:859833/1‑150 (MQ=255)
aGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTAt > 1:197095/1‑151 (MQ=255)
gAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCg < 2:315861/151‑1 (MQ=255)
ccGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAgtgt < 2:730734/151‑1 (MQ=255)
aGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGc < 1:839861/151‑1 (MQ=255)
ggAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGcc < 2:902598/151‑1 (MQ=255)
aaaTGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGcc > 2:722622/1‑149 (MQ=255)
aaCCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGac < 1:339261/151‑1 (MQ=255)
aCCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGaca < 1:159866/151‑1 (MQ=255)
ccTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGacac > 1:128184/1‑151 (MQ=255)
gCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACttttt < 2:900323/151‑1 (MQ=255)
gCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACtttt < 1:15663/150‑1 (MQ=255)
gCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACtttt < 2:555968/150‑1 (MQ=255)
gCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACtt < 1:98008/148‑1 (MQ=255)
atcatcAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTG‑TTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTtgtg < 2:682504/151‑1 (MQ=255)
ttGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACgg > 2:853108/1‑150 (MQ=255)
ccTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGtt < 2:816830/151‑1 (MQ=255)
ctgccTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATAGCGATAGGGACATGAAGCGTTCTGTTCATAAAGCCTGCTGTTTTATTGTCCGCATAAAGGGTAACGCCAGATGACGCTTTTTGTGTAATGACGGAGtta < 2:247674/146‑2 (MQ=255)
ctTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTc < 2:706939/149‑1 (MQ=255)
ttCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTcac > 1:204416/1‑150 (MQ=255)
tCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTcaca < 2:31784/150‑1 (MQ=255)
tCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTca < 2:909762/148‑1 (MQ=255)
gCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAg < 1:640120/151‑1 (MQ=255)
aaTCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGa < 2:902337/149‑1 (MQ=255)
aaTCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATc < 1:263650/151‑1 (MQ=255)
aTCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATc > 2:97427/1‑150 (MQ=255)
tCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCa > 1:219529/1‑150 (MQ=255)
cACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCaa < 1:92547/150‑1 (MQ=255)
cgcgTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAagg > 2:632380/1‑151 (MQ=255)
tAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAAt > 2:616838/1‑150 (MQ=255)
aaTTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATaa < 1:811732/151‑1 (MQ=255)
aTTCTGAATCTGGAATTTCGATAGTGACAAGAATCGTTCTGTTCATAAAGCCACCTGTTTTAGTATCCGTATAAAGTGTAACTCCAGATGACACTTTTTGTTTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGTAGGAAGAATaa < 2:746688/150‑1 (MQ=255)
gAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGa < 1:655867/150‑1 (MQ=255)
aaTCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGaaa < 1:632429/151‑1 (MQ=255)
aaTCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGaaa < 1:180151/151‑1 (MQ=255)
aTCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGaaaa < 1:701562/151‑1 (MQ=255)
cGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATGAAGTGTAACGCCAGATGACACGTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGTGGAAGAGTAAGCACaaaaa < 2:492394/150‑1 (MQ=255)
atCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGcc > 2:671012/1‑150 (MQ=255)
atCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGcc > 2:523548/1‑150 (MQ=255)
ggACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCg > 2:636204/1‑151 (MQ=255)
aaGAATCTTTCTGTTCATAAACCCTCTTTTTTTAGTACCCGAAAAAAGTGAACCGCCAGATGACATTTTTTGTGTAATGACGGATTTCACTTTTTTAATTAAGAAAAAAGGAGAAAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCa < 2:802743/151‑1 (MQ=255)
cGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCaggagg > 1:185827/1‑150 (MQ=255)
ttCTGTTCATAAAGCCCCTTTTTTTAGTATCCTAATAAAGTGTACCGCAAGATGACATTTTTTGTGTAAGGCGGGAGTTCTCATTTTTAATTAAAACAAAAGGAGAAAGAATAAGAAAAAAAAGCCCGCCATAACAGCGGGCaggagg < 2:864787/148‑1 (MQ=255)
gTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGAttt > 1:660488/1‑148 (MQ=255)
gTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGAttt > 1:260425/1‑148 (MQ=255)
ttCATAAACCCTCTTGTTTTAGTATCCGAATAAAGTGTAACGCAAGATGACATTTTTTGTGTAATGAGGGAGTTCACATTTTTAATTAAGAAAAAAGGAGGAAAAAAAAGCAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGa < 2:128162/150‑1 (MQ=255)
tCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGaa > 2:401513/1‑150 (MQ=255)
aaagCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGa > 1:694887/1‑150 (MQ=255)
|
AGGCATAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGA > NC_000913/1435475‑1435769
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
