Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F50 I2 R1
|
257 |
49.9 |
1554294 |
99.0% |
1538751 |
150.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,435,623 |
A→G |
intergenic (‑4/+137) |
uspF ← / ← ompN |
stress‑induced protein, ATP‑binding protein/outer membrane pore protein N, non‑specific |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,435,623 | 0 | A | G | 94.1%
| 177.5
/ ‑6.1
| 51 | intergenic (‑4/+137) | uspF/ompN | stress‑induced protein, ATP‑binding protein/outer membrane pore protein N, non‑specific |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (25/23); minor base C (0/3); total (25/26) |
| Fisher's exact test for biased strand distribution p-value = 2.35e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTG > NC_000913/1435480‑1435768
|
tagtagGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGACAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTg > 2:149103/1‑151 (MQ=255)
agGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGttt < 2:536386/150‑1 (MQ=255)
gggCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTa < 2:600690/151‑1 (MQ=255)
gCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGt < 1:723255/151‑1 (MQ=255)
gTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATc < 2:90977/151‑1 (MQ=255)
aaGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGc > 1:318159/1‑151 (MQ=255)
aaGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGc > 1:382542/1‑151 (MQ=255)
ggTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCAt < 1:519542/151‑1 (MQ=255)
aTTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATaaa < 1:674251/151‑1 (MQ=255)
tACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAg < 2:2485/150‑1 (MQ=255)
aGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGc < 1:138573/151‑1 (MQ=255)
aaaTGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGcc > 2:450066/1‑149 (MQ=255)
aaTGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGcc > 1:696019/1‑148 (MQ=255)
gAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATg < 2:318018/150‑1 (MQ=255)
aaCCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGac < 2:56871/151‑1 (MQ=255)
gCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACttttt < 2:169529/151‑1 (MQ=255)
gCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACttt < 2:211332/149‑1 (MQ=255)
atcatcAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTtgt < 2:247604/151‑1 (MQ=255)
tcatcaATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTtgtg > 1:246360/1‑151 (MQ=255)
tcaATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTa > 2:453321/1‑150 (MQ=255)
aaTCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATg < 2:674799/151‑1 (MQ=255)
ttGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACgg > 2:98919/1‑150 (MQ=255)
tGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACgg > 1:313135/1‑149 (MQ=255)
ccTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGtt > 2:537460/1‑151 (MQ=255)
ctTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTcac > 1:520272/1‑151 (MQ=255)
tCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGTAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACTCCAGATGACACTTTTTGTGTAATGACGGAGTTca < 2:78867/148‑1 (MQ=255)
tCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACAt > 1:34309/1‑151 (MQ=255)
cAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACAttttt > 2:371257/1‑151 (MQ=255)
tGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAAttt > 2:208291/1‑151 (MQ=255)
ggCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTa < 2:16216/151‑1 (MQ=255)
gCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAg < 2:313168/151‑1 (MQ=255)
aaTCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGa < 1:721596/149‑1 (MQ=255)
aCGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAag < 2:182169/151‑1 (MQ=255)
aCGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAag < 2:117630/151‑1 (MQ=255)
gTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAaggagg > 2:508079/1‑151 (MQ=255)
gAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAggagga > 2:397646/1‑149 (MQ=255)
aGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGgaaga < 2:728446/151‑1 (MQ=255)
aaTCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGaa > 2:580704/1‑150 (MQ=255)
cGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGaaaa > 2:348869/1‑149 (MQ=255)
aaTATCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCg < 2:246393/151‑1 (MQ=255)
atatCGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGc > 1:480634/1‑151 (MQ=255)
cGATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCAt > 1:634443/1‑150 (MQ=255)
gATAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATa > 2:95035/1‑150 (MQ=255)
aTAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAAc > 2:411455/1‑151 (MQ=255)
tAGGGACAAGAATCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAAc > 2:82755/1‑150 (MQ=255)
attctttttGTTAAAAAACCCTCTTTTTTTAGTATCCAAATAAAGTTTAACGCCAAATGACATTTTTTGTGTAAGAACGGATTCCACTTTTTTAATTAAGAACAAAGGGGAAAAAATAAGAAAAAAAAGCCCGCCATAACAGCGGGCag < 1:533491/141‑1 (MQ=255)
aTCGTTCTGTTCATAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCagg > 1:6654/1‑149 (MQ=255)
tctttCTTTTAAAAAACCCTCTTTTTTTAGTACCCAAAAAAAGTGTAACCCAAGATGACTTTTTTTGTGAAAGGACGGATTTCACTTTTTTAATTAAGAAAAAGGGAGAAAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCaggag < 1:397597/147‑1 (MQ=255)
ttttttaaaaaaagCCTCTTTTTTTATTATCCAAAAAAGTTGTAACGCAAGTTGATTGTTTTTGAGAAATGAGGGATTTCAATTTTTTAATTTAGAGAAAGGGAGAAAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCaggagg < 1:249453/138‑1 (MQ=255)
ttttGTTAAAAAACCCTCTTTTTTTAGTACCCGAAAAAAGTGAAACGCAAGAGGACATTTTTTGTGTAATGGCGGAGTCCACATTTTTAATTTAGATCAAGGGAGGAAGAATAAGAAGAAAAAGCCCGCCATAACAGCGGGCaggag < 2:318192/144‑1 (MQ=255)
ttCTGTTAAAAAAGCCTCTTTTTTTAGTACCCGCATAAAGTGAACCGCCAGATGACATTTTTTGTGTAATGGCGAATTCCACTTTTTAAATTTAGAAAAAAGGAGAAAAAATAAGCAAAAAAAGCCCGCCATAACAGCGGGCaggagg < 1:182821/148‑1 (MQ=255)
aTAAAGCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTg > 1:100456/1‑151 (MQ=255)
|
TAGTAGGGCAGTGAAGGTATTACCGTCAGGAAATGAACCTCTGCATCATCAATCTTTGCCTCTTCCTCAACGTGGCTAATCACGCGTTGAGTTAATTCTGAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTG > NC_000913/1435480‑1435768
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
