Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I3 R1 283 58.3 1819865 98.8% 1798026 150.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,790,415 T→C V145A (GTC→GCC)  lhgO → L‑2‑hydroxyglutarate oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,790,4150TC98.0% 180.3 / NA 49V145A (GTC→GCC) lhgOL‑2‑hydroxyglutarate oxidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (22/26);  minor base A (1/0);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTA‑TAACGCCGAAGTCA‑GCGGGCTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAA  >  NC_000913/2790269‑2790557
                                                                                                                                                  |                                                                                                                                                
tctcGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGc                                                                                                                                              >  1:887489/1‑151 (MQ=255)
          gAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACg                                                                                                                                    <  1:721445/151‑1 (MQ=255)
           aaCGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGAc                                                                                                                                     >  2:568782/1‑149 (MQ=255)
              ggATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGACAGCTACCGCGACGTg                                                                                                                                  >  1:540598/1‑149 (MQ=255)
                   cgcgcCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGAcggcgg                                                                                                                           <  1:634091/151‑1 (MQ=255)
                   cgcgcCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGAcggcgg                                                                                                                           <  1:56451/151‑1 (MQ=255)
                         ttATGGGAACGCCCAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCCGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGCTg                                                                                                                      >  2:10990/1‑150 (MQ=255)
                              ggAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCaaaa                                                                                                                <  2:887049/151‑1 (MQ=255)
                               gAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCaaaaa                                                                                                               <  1:488294/151‑1 (MQ=255)
                                 aCGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATc                                                                                                             <  2:365354/151‑1 (MQ=255)
                                   gCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCtt                                                                                                           <  1:317293/151‑1 (MQ=255)
                                              aaCGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTtccagtccag                                                                                                  >  2:809059/1‑149 (MQ=255)
                                                cGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGc                                                                                              <  2:647591/151‑1 (MQ=255)
                                                    aTCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTg                                                                                          <  2:331050/151‑1 (MQ=255)
                                                    aTCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTg                                                                                          >  2:536303/1‑151 (MQ=255)
                                                      cGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGaa                                                                                        <  1:498596/151‑1 (MQ=255)
                                                      cGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGa                                                                                         <  2:393048/150‑1 (MQ=255)
                                                       gAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCgggg                                                                                          >  1:83920/1‑146 (MQ=255)
                                                             gAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCt                                                                                 <  1:371029/151‑1 (MQ=255)
                                                               aTGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCta‑t                                                                              <  2:373457/151‑1 (MQ=255)
                                                               aTGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCta‑t                                                                              <  1:786139/151‑1 (MQ=255)
                                                                tGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGGGaaa                                                                                       >  2:325164/1‑142 (MQ=255)
                                                                 ggCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCta‑ta                                                                             <  2:876731/150‑1 (MQ=255)
                                                                  gCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTA‑Taa                                                                            >  2:96487/1‑150 (MQ=255)
                                                                       aCGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTA‑TAACGCCGa                                                                      <  1:406517/151‑1 (MQ=255)
                                                                             acgaACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGGAAATTTTTA‑TAAAGcc                                                                        >  1:473708/1‑143 (MQ=255)
                                                                                  cTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAAATATCTA‑TAAAGCCGAAGTCA‑GCggg                                                           >  2:307427/1‑150 (MQ=255)
                                                                                    gcgcgagcgcgaACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTA‑TAACGCCGAAGTCA‑GCGGGCtt                                                        <  1:522922/151‑1 (MQ=255)
                                                                                    gcgcgagcgcgaACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGAGAAATTATCTA‑TAACGCCGAAGTCA‑GCGGGCtt                                                        >  1:619525/1‑151 (MQ=255)
                                                                                     cgcgagcgcgaACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAAATATCTA‑TAAAGCCGAAGGCA‑GCGGGCtt                                                        >  1:720531/1‑150 (MQ=255)
                                                                                      gcgagcgcgaACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGGAAATATATA‑TAAAGCCGAAGTCA‑GGGGGCt                                                         >  1:368691/1‑148 (MQ=255)
                                                                                      gcgagcgcgaACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAAATATCTA‑TAAAGGCGAAAGCA‑GAGGGCtt                                                        >  1:490348/1‑149 (MQ=255)
                                                                                         agcgcgaACCGAATATCACCGGCCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAACTCTTCCAGTCCAGAGGAGGTGGAAATCTTTACTAACCCCGAAGtagctagggtt                                                         >  2:47600/1‑137 (MQ=255)
                                                                                          gcgcgaACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTA‑TAACGCCGAAGTCA‑GCGGGCTTAATGa                                                   <  2:719711/150‑1 (MQ=255)
                                                                                           cgcgaACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTA‑TAACGCCGAAGTCA‑GCGGGCTTAATGAGc                                                 <  2:838910/151‑1 (MQ=255)
                                                                                                       atCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTA‑TAACGCCGAAGTCA‑GCGGGCTTAATGAGCATAAAAACGGc                                      <  1:395411/150‑1 (MQ=255)
                                                                                                       atCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAAATTTCTA‑TAAAGCCGAAATCA‑GGGGGGTTAATGAGCATAAAAAcag                                       >  2:514228/1‑147 (MQ=255)
                                                                                                               gCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGGAAATATCTT‑TAACGCCCAAGACA‑ACGGGCTTAATGAGCATaaaaaaagcgggggggt                              >  2:530852/1‑138 (MQ=255)
                                                                                                                cTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGGTTAATTTTTTT‑TAACGCCGCAGGCA‑GCGGGGCTGATAAGCATaaaaaacgggggggggtac                            >  2:289277/1‑137 (MQ=255)
                                                                                                                  cggcggCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTA‑TAACGCCGAAGTCA‑GCGGGCTTAATGAGCATAAAAACGGCGTGGTGATAc                            <  2:523842/149‑1 (MQ=255)
                                                                                                                  cggcggCATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTA‑TAACGCCGAAGTCA‑GCGGGCTTAATGAGCATAAAAACGGCGTGGTGATACg                           <  2:876604/150‑1 (MQ=255)
                                                                                                                       gcATTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTA‑TAACGCCGAAGTCA‑GCGGGCTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCg                     <  1:416430/151‑1 (MQ=255)
                                                                                                                         aTTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGTAATTATCTT‑TAAAGCCGCAGTCA‑GCGGGCTTAATTGGCATAAAAAAAGCGGGGGGGTACGTACCCGTc                   >  1:663292/1‑151 (MQ=255)
                                                                                                                         aTTTTTGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGATAAATTATCTA‑ATAAGCCGGAATCACGCGGCATGAAT‑AGCATAAAAACGGCGTGGGGGTACATCCCCCTc                   >  1:229268/1‑151 (MQ=255)
                                                                                                                           ttttGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTA‑TAACGCCGAAGTCC‑GCGGGCTTAATGAGCATAAAAAAGGCGTGGGGATACGTAccccgc                   >  1:291368/1‑146 (MQ=255)
                                                                                                                            tttGTGCCGTCCAGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTA‑TAACGCCGAAGTCA‑GCGGGCTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAg                 <  1:875140/150‑1 (MQ=255)
                                                                                                                                        aGCGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTA‑TAACGCCGAAGTCA‑GCGGGCTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATg    <  1:242277/151‑1 (MQ=255)
                                                                                                                                          cGGCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTA‑TAACGCCGAAGTCA‑GCGGGCTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGaa  <  2:728003/151‑1 (MQ=255)
                                                                                                                                            gCATTGCCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGGAATTTTCTA‑TAAAGCCGCAGTTA‑GCGGGCTTTATTAGCATAAAAAAGGCGTGGGGGTACGTACCCCTCCGGGCGGCGGatat     >  1:315441/1‑146 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTGAAATTATCTA‑TAACGCCGAAGTCA‑GCGGGCTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAA  >  NC_000913/2790269‑2790557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A