Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I3 R1	283	58.3	1819865	98.8%	1798026	150.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,837,983	T→C	L41L (TTA→TTG)	hydN ←	formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,837,983	0	T	C	100.0%	165.3 / NA	49	L41L (TTA→TTG)	hydN	formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (20/29); total (20/29)

GCAACGTTCCTGCATCACATGAACAAACCCTTTATCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGC  >  NC_000913/2837839‑2838118

gCAACGTTCCTGCATCACATGAACAAACCCTTTATCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCaaaaaa                                                                                                                                   <  2:666975/151‑1 (MQ=255)

gCAACGTTCCTGCATCACATGAACAAACCCTTTATCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCaaaaaa                                                                                                                                   <  2:645424/151‑1 (MQ=255)

      ttCCTGCATCACATGAACAAACCCTTTATCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTcc                                                                                                                             >  2:322787/1‑151 (MQ=255)

        ccTGCATCACATGAACAAACCCTTTATCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCgg                                                                                                                           <  1:22270/151‑1 (MQ=255)

         cTGCATCACATGAACAAACCCTTTATCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCggg                                                                                                                          >  2:874767/1‑151 (MQ=255)

            cATCACATGAACAAACCCTTTATCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTc                                                                                                                       <  1:427136/151‑1 (MQ=255)

               cacaTGAACAAACCCTTTATCACGGCTGATAGCCCCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACGCCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCggg                                                                                                                          <  2:535445/145‑1 (MQ=255)

               cacaTGAACAAACCCTTTATCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAg                                                                                                                     <  2:647794/150‑1 (MQ=255)

                acaTGAACAAACCCTTTATCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGc                                                                                                                    <  2:508995/150‑1 (MQ=255)

                    gAACAAACCCTTTATCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGc                                                                                                               >  1:116910/1‑151 (MQ=255)

                     aaCAAACCCTTTATCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGc                                                                                                               >  1:887538/1‑150 (MQ=255)

                      aCAAACCCTTTATCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGcac                                                                                                             <  2:127430/151‑1 (MQ=255)

                          aCCCTTTATCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGTTGCATCTTCACACTGACGGCAAACTGTAGCCGTGGAAATGTTCACACCTTTAATGACATGGAAACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGt                                                                                                          >  2:151495/1‑150 (MQ=255)

                              tttATCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTg                                                                                                      >  1:870952/1‑150 (MQ=255)

                                  tCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGTAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGAtttt                                                                                                 >  1:553621/1‑151 (MQ=255)

                                   cACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTc                                                                                                <  1:461897/151‑1 (MQ=255)

                                    aCGGCTGATAGCGCCAAGCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAATTTTCCGGGGTCAGCGATGCACAGTCCTGAtt                                                                                                   <  2:145591/147‑1 (MQ=255)

                                              gCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGaa                                                                                      >  2:688936/1‑150 (MQ=255)

                                               cACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAAc                                                                                    <  2:456633/151‑1 (MQ=255)

                                                       gggCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACcacgcacg                                                                            >  2:639725/1‑151 (MQ=255)

                                                         gCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATATTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCt                                                                          <  2:280341/151‑1 (MQ=255)

                                                          cAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCt                                                                          <  1:495672/150‑1 (MQ=255)

                                                           aGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGTTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTAc                                                                        <  2:824746/151‑1 (MQ=255)

                                                             aCGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACtt                                                                      <  2:583051/151‑1 (MQ=255)

                                                                     gcACGGTGCATCTTCACACTGCCGGCAAACCGTCGCCGTGGAAATGTTCACACCTTTAATGCCATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAACCCACGCACGCTACTTCACAGGTa                                                              <  2:381700/151‑1 (MQ=255)

                                                                        cGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACgg                                                           <  1:17671/151‑1 (MQ=255)

                                                                          gTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGc                                                          >  1:330619/1‑150 (MQ=255)

                                                                          gTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCa                                                         <  2:224524/151‑1 (MQ=255)

                                                                                   cacaCTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATAc                                                 <  1:597381/150‑1 (MQ=255)

                                                                                    acacTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACAt                                               >  2:39902/1‑151 (MQ=255)

                                                                                         gACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTAc                                           <  1:753992/150‑1 (MQ=255)

                                                                                              cAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGt                                     <  1:858353/151‑1 (MQ=255)

                                                                                                  cTGTCGCCGTGGAAATGTTCACACCTTCAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGc                                 >  1:37574/1‑151 (MQ=255)

                                                                                                    gTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCa                                >  1:272689/1‑150 (MQ=255)

                                                                                                    gTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCa                                <  1:618115/150‑1 (MQ=255)

                                                                                                         cGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAatgatg                          <  2:539454/151‑1 (MQ=255)

                                                                                                            ggAAATGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGaa                        >  2:457648/1‑150 (MQ=255)

                                                                                                                 tGTTCACACCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGtt                  >  1:439170/1‑151 (MQ=255)

                                                                                                                     cacaCCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtcat               <  2:617975/150‑1 (MQ=255)

                                                                                                                       cacCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCaa            <  2:781399/151‑1 (MQ=255)

                                                                                                                        acCTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGTCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCaaa           <  1:692663/151‑1 (MQ=255)

                                                                                                                          cTTTAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAAtt         >  1:693608/1‑151 (MQ=255)

                                                                                                                           tttAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGATACTTAACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACTGTTCATCAAAt          >  2:782065/1‑149 (MQ=255)

                                                                                                                            ttAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtc        <  2:801737/150‑1 (MQ=255)

                                                                                                                             tAATGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtct       <  2:888400/150‑1 (MQ=255)

                                                                                                                               aTGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTcc     >  1:572008/1‑150 (MQ=255)

                                                                                                                               aTGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTcc     >  1:667901/1‑150 (MQ=255)

                                                                                                                               aTGACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCa    <  1:252014/151‑1 (MQ=255)

                                                                                                                                 gACATGGATACGCGGCAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGc  >  1:545891/1‑151 (MQ=255)

GCAACGTTCCTGCATCACATGAACAAACCCTTTATCACGGCTGATAGCACCATTCGGGCAGACGTTAGCGCACGGTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGC  >  NC_000913/2837839‑2838118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A