Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F50 I2 R1
|
257 |
49.9 |
1554294 |
99.0% |
1538751 |
150.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,849,543 |
G→A |
R103C (CGT→TGT) |
hycB ← |
hydrogenase 3, Fe‑S subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,849,543 | 0 | G | A | 100.0%
| 120.9
/ NA
| 34 | R103C (CGT→TGT) | hycB | hydrogenase 3, Fe‑S subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/13); total (21/13) |
ATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAGGGTGCATCTT > NC_000913/2849403‑2849686
|
atcaAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGa > 2:598887/1‑150 (MQ=255)
tACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTTGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCaa > 1:693710/1‑150 (MQ=255)
aCAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAAtt < 1:595897/151‑1 (MQ=255)
cAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTg < 1:374981/151‑1 (MQ=255)
cAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGGGGCGCTTTCCGGGGATTGGCGTTTTCCGGAATATCCAGCGGACACCTGCCGGAAAATTCAAtt > 1:333510/1‑150 (MQ=255)
aTTTGAAGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGAGCCGGTGGGGCTTTAGGGGGATTGGGGTTTGCCGGAATATCCAGCGGACAGATGCCGGAAAATTCAATTGCG‑CCAAACg > 1:605561/1‑150 (MQ=255)
tttGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACggg > 2:419450/1‑151 (MQ=255)
cGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGa > 2:512777/1‑150 (MQ=255)
gATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCCTTCGGGGTATTCGCGTTTGCCGGGATTTCCAGCGGACAGATGCCGGCAAATTCAATTGCGCCCAAACGGCCAgcggtg > 1:145691/1‑146 (MQ=255)
gcgcgAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGcc > 1:476049/1‑149 (MQ=255)
cgcgAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGca < 2:575363/151‑1 (MQ=255)
cgcgAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGc > 2:366748/1‑150 (MQ=255)
gAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGcaca < 1:667242/150‑1 (MQ=255)
tACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGGTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGcac > 1:456310/1‑146 (MQ=255)
cTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTg > 1:633476/1‑149 (MQ=255)
aaGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACAGCCGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGcaca > 1:447423/1‑149 (MQ=255)
gCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCt < 2:397908/151‑1 (MQ=255)
aTGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTttc < 1:421455/151‑1 (MQ=255)
gCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTttcattc > 2:746118/1‑151 (MQ=255)
gCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTttcatt > 1:477538/1‑150 (MQ=255)
cgcgCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCa < 1:373891/151‑1 (MQ=255)
gcCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACgg < 1:51953/151‑1 (MQ=255)
gcCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACgg > 2:710730/1‑151 (MQ=255)
aGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGcccc > 2:212730/1‑150 (MQ=255)
aGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCg > 1:202980/1‑151 (MQ=255)
gTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGAcgcg > 2:197837/1‑151 (MQ=255)
ttCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGtt < 1:615507/149‑1 (MQ=255)
ttCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGtt < 2:21036/149‑1 (MQ=255)
ggggTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCg < 1:450290/151‑1 (MQ=255)
tATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGc > 1:366436/1‑150 (MQ=255)
aTTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGc > 1:267320/1‑149 (MQ=255)
ttGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAAACGCGCAGACCACcg < 1:431519/150‑1 (MQ=255)
atatCCAGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAGGGTg < 1:128401/151‑1 (MQ=255)
aGCGGACAGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAGGGTGCATCtt > 1:490406/1‑151 (MQ=255)
|
ATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCG‑CCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAGGGTGCATCTT > NC_000913/2849403‑2849686
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
