Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I3 R1	283	58.3	1819865	98.8%	1798026	150.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,849,543	G→A	R103C (CGT→TGT)	hycB ←	hydrogenase 3, Fe‑S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,849,543	0	G	A	100.0%	165.3 / NA	46	R103C (CGT→TGT)	hycB	hydrogenase 3, Fe‑S subunit
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (26/20); total (26/20)

TCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAGGGTGCATCTTCAC  >  NC_000913/2849401‑2849689

tcatcaAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCCTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACAGCTTCCgg                                                                                                                                            >  2:378214/1‑151 (MQ=255)

    caAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGaaa                                                                                                                                         <  2:16083/150‑1 (MQ=255)

    caAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGAGACGGCGGCGCTTTCCGGGGAGTGCAGGTTGGCGGAATATCCCGCGGACAGCTGCCgcgaa                                                                                                                                         >  2:488820/1‑146 (MQ=255)

     aaaGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAAt                                                                                                                                       <  2:598771/151‑1 (MQ=255)

          tACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATAACCAGCGGACAGCTGCCGGAAAATTCaa                                                                                                                                   >  2:801111/1‑150 (MQ=255)

           aCAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAAtt                                                                                                                                 <  2:83599/151‑1 (MQ=255)

           aCAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGGTTGCCGGGATATCCAGCGGGCAGCTGCCGGAAAATTCAAtt                                                                                                                                 >  2:15488/1‑151 (MQ=255)

                   cacaTTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCaa                                                                                                                          >  1:371703/1‑150 (MQ=255)

                    acaTTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCaaa                                                                                                                         >  1:209873/1‑150 (MQ=255)

                        ttGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGCGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATAACCAGCGGACAGCTGCCGGGAAATTCAATTGCGCCAAACggg                                                                                                                     >  2:469627/1‑150 (MQ=255)

                          gACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCTGAAAATTCAATTGCGCCAAACGGGCa                                                                                                                   <  2:763700/150‑1 (MQ=255)

                           aCGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAgg                                                                                                                 >  1:415765/1‑151 (MQ=255)

                            cGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGc                                                                                                                <  2:138214/151‑1 (MQ=255)

                                       cgAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGcac                                                                                                      >  1:789544/1‑150 (MQ=255)

                                       cgAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGGGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGca                                                                                                       >  2:874917/1‑149 (MQ=255)

                                         aaTACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGc                                                                                                   >  1:809547/1‑151 (MQ=255)

                                               tGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGAGATGCCGCACAGCTTGc                                                                                               >  1:544490/1‑149 (MQ=255)

                                                ggCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAg                                                                                             <  1:802163/150‑1 (MQ=255)

                                                    cccAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTAc                                                                                        >  1:392874/1‑151 (MQ=255)

                                                       aGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGca                                                                                     <  1:509343/151‑1 (MQ=255)

                                                                    gCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTttcatt                                                                         <  1:592717/150‑1 (MQ=255)

                                                                        acacGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCCTACGCACAGGCCTTCATTCaa                                                                      >  2:54020/1‑149 (MQ=255)

                                                                              gcCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACATGCTTTTATTCAACTGCa                                                                 >  2:566915/1‑148 (MQ=255)

                                                                                     cAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATc                                                       >  2:650056/1‑151 (MQ=255)

                                                                                                 gTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGAt                                           >  1:685591/1‑151 (MQ=255)

                                                                                                 gTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGAt                                           >  1:320010/1‑151 (MQ=255)

                                                                                                         ttCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAGACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTa                                    <  2:276508/150‑1 (MQ=255)

                                                                                                          tCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTaa                                   <  1:805469/150‑1 (MQ=255)

                                                                                                           cGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAAcc                                 >  1:25230/1‑151 (MQ=255)

                                                                                                           cGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAAcc                                 >  1:897903/1‑151 (MQ=255)

                                                                                                              ggTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCggg                              <  1:372489/151‑1 (MQ=255)

                                                                                                               gTATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGc                             <  1:622927/151‑1 (MQ=255)

                                                                                                                tATTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGc                             >  2:903330/1‑150 (MQ=255)

                                                                                                                 aTTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCa                            <  2:807036/150‑1 (MQ=255)

                                                                                                                 aTTGGCGTTTGCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCa                            <  2:421128/150‑1 (MQ=255)

                                                                                                                           gCCGGAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACcgcg                  <  2:421079/150‑1 (MQ=255)

                                                                                                                              ggAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAgg              <  1:632735/151‑1 (MQ=255)

                                                                                                                               gAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAggg             <  1:55668/151‑1 (MQ=255)

                                                                                                                               gAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAggg             >  1:464333/1‑151 (MQ=255)

                                                                                                                               gAATATCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAggg             <  2:789493/151‑1 (MQ=255)

                                                                                                                                  tatCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAGGGTGc          >  1:444792/1‑151 (MQ=255)

                                                                                                                                   atCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAGGGTgaa         <  2:588778/151‑3 (MQ=255)

                                                                                                                                   atCCAGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAGGGTGc          >  2:854592/1‑150 (MQ=255)

                                                                                                                                       aGCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAGGGTGCATCtt     >  1:537931/1‑151 (MQ=255)

                                                                                                                                        gCGGACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAGGGTGCATCTTc    <  1:91950/151‑1 (MQ=255)

                                                                                                                                           gACAGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAGGGTGCATCTTcac  >  2:425427/1‑150 (MQ=255)

TCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAGGGTGCATCTTCAC  >  NC_000913/2849401‑2849689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A