Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I3 R1 283 58.3 1819865 98.8% 1798026 150.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,118,599 C→T V172I (GTT→ATT)  sslE ← putative secreted and surface‑associated lipoprotein mucinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,118,5990CT100.0% 199.6 / NA 55V172I (GTT→ATT) sslEputative secreted and surface‑associated lipoprotein mucinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/30);  total (25/30)

CCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAG  >  NC_000913/3118458‑3118742
                                                                                                                                             |                                                                                                                                               
ccAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCt                                                                                                                                        >  2:812825/1‑151 (MQ=255)
  aGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCttt                                                                                                                                      <  1:795280/151‑1 (MQ=255)
   gCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCGGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCtttt                                                                                                                                     <  1:888257/151‑1 (MQ=255)
   gCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCtttt                                                                                                                                     >  1:832410/1‑151 (MQ=255)
   gCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCtttt                                                                                                                                     <  1:273746/151‑1 (MQ=255)
       tttGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCtt                                                                                                                                  <  2:844514/150‑1 (MQ=255)
        ttGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTg                                                                                                                                 <  2:381972/150‑1 (MQ=255)
        ttGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGt                                                                                                                                >  2:803981/1‑151 (MQ=255)
          gAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTc                                                                                                                               <  2:613083/150‑1 (MQ=255)
               ctTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCg                                                                                                                           <  1:274759/149‑1 (MQ=255)
                 tCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGa                                                                                                                         <  1:885501/149‑1 (MQ=255)
                   cGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGccc                                                                                                                     <  2:810152/151‑1 (MQ=255)
                     gTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGc                                                                                                                   <  1:707592/151‑1 (MQ=255)
                       gCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGcc                                                                                                                  >  2:699565/1‑150 (MQ=255)
                           gATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTc                                                                                                             >  2:779728/1‑151 (MQ=255)
                              cGATTTGCTTATACAGCGAGTCGAAGCGTTTCCTCTCGATCACCGAGGAGAACGTCAGACAACCCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTc                                                                                                             <  1:544544/148‑1 (MQ=255)
                                aTTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcg                                                                                                        <  1:477561/151‑1 (MQ=255)
                                 tttGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcg                                                                                                        >  2:25853/1‑150 (MQ=255)
                                   tGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGt                                                                                                     >  1:527449/1‑151 (MQ=255)
                                       tataCAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGAAGAACGACAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACAAGCGAAATTGCATTGCTTTTCTTTTCATCGAAGCCCGCCAGTTCTTGCGCGTcc                                                                                                   >  2:714790/1‑149 (MQ=255)
                                        ataCAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGCAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTc                                                                                                 >  2:553222/1‑150 (MQ=255)
                                        ataCAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCa                                                                                                <  2:758811/151‑1 (MQ=255)
                                        ataCAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCa                                                                                                <  1:28428/151‑1 (MQ=255)
                                           cAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAgg                                                                                             <  2:308662/151‑1 (MQ=255)
                                           cAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAgg                                                                                             >  2:368534/1‑151 (MQ=255)
                                              cGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCt                                                                                           >  1:899745/1‑150 (MQ=255)
                                                        cGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACtt                                                                                >  2:318450/1‑151 (MQ=255)
                                                         gTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACtt                                                                                <  2:206520/150‑1 (MQ=255)
                                                            tACTCTCGATCCCCGAGGAGACCGTCAGACAAACCTGTTCTGTATTCGCCGGACACCTGTTATGGGCCGTTACCAGCAAACTCGTTTCGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACttttt                                                                             <  2:153615/150‑1 (MQ=255)
                                                             aCTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCa                                                                           <  2:630735/151‑1 (MQ=255)
                                                              ctctCGATCACCGAGGCCAACGTCAGACAACCCTGTTCTGTTTTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCTTTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTc                                                                            <  2:66591/149‑1 (MQ=255)
                                                                  cGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAAcc                                                                        <  2:358373/149‑1 (MQ=255)
                                                                     tCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACcgcac                                                                    <  2:254664/150‑1 (MQ=255)
                                                                            ggagAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCt                                                             >  2:80847/1‑150 (MQ=255)
                                                                            ggagAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCt                                                             <  2:887125/150‑1 (MQ=255)
                                                                               gaACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTAcg                                                          <  2:818894/150‑1 (MQ=255)
                                                                               gaACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTAcg                                                          <  1:521380/150‑1 (MQ=255)
                                                                                caCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTAcgc                                                         >  1:796756/2‑150 (MQ=255)
                                                                                        aCAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTct                                                <  1:961/151‑1 (MQ=255)
                                                                                           aaCCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTctgac                                             >  2:505067/1‑151 (MQ=255)
                                                                                                gTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAgt                                        <  1:368560/151‑1 (MQ=255)
                                                                                                 ttCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAGCGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAg                                         >  2:700199/1‑149 (MQ=255)
                                                                                                  tCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAgtg                                       >  1:575789/1‑150 (MQ=255)
                                                                                                  tCTGGATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAgtgt                                      >  2:542651/1‑151 (MQ=255)
                                                                                                    tGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTg                                    >  2:870252/1‑151 (MQ=255)
                                                                                                      tATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGtt                                     <  2:880500/148‑1 (MQ=255)
                                                                                                       aTTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAg                                 <  1:203198/151‑1 (MQ=255)
                                                                                                             cGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGc                            >  1:57186/1‑150 (MQ=255)
                                                                                                                aCAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAAtt                        <  1:310713/151‑1 (MQ=255)
                                                                                                                 cAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTg                       >  1:722842/1‑151 (MQ=255)
                                                                                                                         aCTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGtt                >  1:134759/1‑150 (MQ=255)
                                                                                                                                gTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGcc         >  1:431986/1‑150 (MQ=255)
                                                                                                                                 ttACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCAc       >  2:487923/1‑151 (MQ=255)
                                                                                                                                   aCCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGc     >  1:68071/1‑151 (MQ=255)
                                                                                                                                      aGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAg  <  1:39153/151‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                               
CCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAG  >  NC_000913/3118458‑3118742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A