Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,118,599	C→T	V172I (GTT→ATT)	sslE ←	putative secreted and surface‑associated lipoprotein mucinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,118,599	0	C	T	100.0%	174.9 / NA	50	V172I (GTT→ATT)	sslE	putative secreted and surface‑associated lipoprotein mucinase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (31/19); total (31/19)

CATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAGTAAC  >  NC_000913/3118452‑3118746

cATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCg                                                                                                                                                   <  1:844070/150‑1 (MQ=255)

   tGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCAtt                                                                                                                                               >  2:21638/1‑151 (MQ=255)

     aCCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGc                                                                                                                                             <  2:259410/151‑1 (MQ=255)

       cAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCtt                                                                                                                                           <  1:573459/151‑1 (MQ=255)

           ttttgGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGATAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTc                                                                                                                                        >  1:618233/1‑150 (MQ=255)

            ttttGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTc                                                                                                                                        <  2:231461/149‑1 (MQ=255)

              ttGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGt                                                                                                                                    >  2:567309/1‑151 (MQ=255)

                gAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTc                                                                                                                                   <  2:149208/150‑1 (MQ=255)

                  aCTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATc                                                                                                                                <  2:298964/151‑1 (MQ=255)

                    tctTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCgg                                                                                                                              >  2:805379/1‑151 (MQ=255)

                        ccGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGc                                                                                                                           <  2:576768/150‑1 (MQ=255)

                           gTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGc                                                                                                                       >  2:608118/1‑151 (MQ=255)

                               aaGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGt                                                                                                                   >  1:741982/2‑151 (MQ=255)

                                 gATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTc                                                                                                                 <  2:386771/151‑1 (MQ=255)

                                     gATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGACCGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgc                                                                                                             >  1:551898/1‑151 (MQ=255)

                                      aTTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcg                                                                                                            >  1:484053/1‑151 (MQ=255)

                                        ttGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcgc                                                                                                           <  2:394517/150‑1 (MQ=255)

                                          gCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGAATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGt                                                                                                         <  2:337880/150‑1 (MQ=255)

                                                aCAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCaa                                                                                                   <  2:733463/150‑1 (MQ=255)

                                                 cAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAgg                                                                                                 >  2:598301/1‑151 (MQ=255)

                                                    cGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCt                                                                                               >  2:855933/1‑150 (MQ=255)

                                                           aaGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGaca                                                                                       <  2:657186/151‑1 (MQ=255)

                                                              cGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACtt                                                                                    >  2:794029/1‑151 (MQ=255)

                                                               gTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACttt                                                                                   >  2:432274/1‑151 (MQ=255)

                                                                  tACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACttttt                                                                                 <  2:71808/150‑1 (MQ=255)

                                                                        cGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAAc                                                                             <  1:491085/148‑1 (MQ=255)

                                                                              ccGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCaa                                                                    <  2:467973/151‑1 (MQ=255)

                                                                                        cGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACgcgc                                                           >  1:14702/1‑150 (MQ=255)

                                                                                         gtaaaaCAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTAcgc                                                             >  1:300130/6‑147 (MQ=255)

                                                                                          tCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGc                                                        >  2:373109/1‑151 (MQ=255)

                                                                                          tCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGc                                                        >  2:465733/1‑151 (MQ=255)

                                                                                           cAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCt                                                       >  2:148467/1‑151 (MQ=255)

                                                                                                aaaCCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTctg                                                   >  2:64229/1‑150 (MQ=255)

                                                                                                aaaCCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTctg                                                   >  2:663290/1‑150 (MQ=255)

                                                                                                 aaCCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTctgac                                                 >  2:671735/1‑151 (MQ=255)

                                                                                                       ttCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAgtg                                           >  1:511/1‑151 (MQ=255)

                                                                                                       ttCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAg                                             <  1:362454/149‑1 (MQ=255)

                                                                                                          tGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTg                                        >  2:647150/1‑151 (MQ=255)

                                                                                                            tATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGa                                       >  1:50980/1‑150 (MQ=255)

                                                                                                              ttCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAgg                                    >  2:445894/1‑151 (MQ=255)

                                                                                                              ttCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAgg                                    >  2:65049/1‑151 (MQ=255)

                                                                                                               tCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGt                                   >  2:342955/1‑151 (MQ=255)

                                                                                                                 gCCGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTgg                                 >  1:295544/1‑151 (MQ=255)

                                                                                                                  ccGGACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGc                                >  2:39289/1‑151 (MQ=255)

                                                                                                                     gACAGCTGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAAt                             <  1:360928/151‑1 (MQ=255)

                                                                                                                           tGTTACTGGACGTTACCAGCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTTAAGGTGGCAATTGTCgt                       >  1:26267/1‑151 (MQ=255)

                                                                                                                                             gCGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAGt     >  1:668275/1‑151 (MQ=255)

                                                                                                                                              cGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAGt     <  2:187397/150‑1 (MQ=255)

                                                                                                                                              cGAAATCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAGt     <  2:483514/150‑1 (MQ=255)

                                                                                                                                                aaaTCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAGTAAc  >  2:561678/1‑151 (MQ=255)

CATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAGTAAC  >  NC_000913/3118452‑3118746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A