Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,265,331	A→G	D107G (GAC→GGC)	prfA →	peptide chain release factor RF‑1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,265,331	0	A	G	100.0%	145.9 / NA	41	D107G (GAC→GGC)	prfA	peptide chain release factor RF‑1
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/22); total (19/22)

CAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTG  >  NC_000913/1265186‑1265474

cAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAAc                                                                                                                                            >  2:494675/1‑151 (MQ=255)

  ggAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACg                                                                                                                                           <  2:37891/150‑1 (MQ=255)

   gAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGt                                                                                                                                          >  1:489171/1‑150 (MQ=255)

            gAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTcc                                                                                                                                <  2:128723/151‑1 (MQ=255)

                     caGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTcc                                                                                                                       <  1:732919/151‑1 (MQ=255)

                      aGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCg                                                                                                                      <  1:170532/151‑1 (MQ=255)

                       gatgatGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCg                                                                                                                      >  2:391969/1‑150 (MQ=255)

                       gatgatGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGa                                                                                                                     <  1:448223/151‑1 (MQ=255)

                        atgatgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAg                                                                                                                    <  1:542900/151‑1 (MQ=255)

                              cTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGa                                                                                                               >  1:364656/1‑150 (MQ=255)

                               tCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAAc                                                                                                             >  1:598437/1‑151 (MQ=255)

                                  atgatCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACcgg                                                                                                          <  1:486741/151‑1 (MQ=255)

                                         tGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGcgac                                                                                                   >  1:323193/1‑151 (MQ=255)

                                          gAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGcgacg                                                                                                  <  1:42341/151‑1 (MQ=255)

                                                  tGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAgcggcg                                                                                          >  1:318976/1‑151 (MQ=255)

                                                     gaTGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTg                                                                                       <  1:80057/151‑1 (MQ=255)

                                                       tGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGtt                                                                                     >  2:500073/1‑151 (MQ=255)

                                                           gcAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTcgcg                                                                                 <  2:410291/151‑1 (MQ=255)

                                                            cAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGGAGCGGCGCTGTTCGCgg                                                                                <  1:10374/151‑1 (MQ=255)

                                                            cAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCgg                                                                                <  2:269172/151‑1 (MQ=255)

                                                              ggATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGc                                                                              <  2:770770/151‑1 (MQ=255)

                                                                aTGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGa                                                                            <  2:660237/151‑1 (MQ=255)

                                                                     cTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGt                                                                       >  1:101034/1‑151 (MQ=255)

                                                                      tGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGtt                                                                      >  1:409667/1‑151 (MQ=255)

                                                                       gcgcGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTc                                                                     >  1:542666/1‑151 (MQ=255)

                                                                        cgcgAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTcc                                                                    <  2:412026/151‑1 (MQ=255)

                                                                            aaGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTAt                                                                <  2:279558/151‑1 (MQ=255)

                                                                             aGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATg                                                               >  2:185564/1‑151 (MQ=255)

                                                                                 aaaGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTAc                                                            >  1:621757/1‑150 (MQ=255)

                                                                                  aaGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAg                                                          <  2:337226/151‑1 (MQ=255)

                                                                                                  aCTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCAGAAGcc                                          <  2:509162/151‑1 (MQ=255)

                                                                                                    tGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCccg                                        >  2:562799/1‑151 (MQ=255)

                                                                                                      gAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCccgcc                                      <  2:105654/151‑1 (MQ=255)

                                                                                                          aGCAATTACAGGTTCTGTTACTGCCACAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTg                                  >  2:543769/1‑151 (MQ=255)

                                                                                                                     gTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGGAGaa                        >  2:410870/1‑150 (MQ=255)

                                                                                                                             aCTGCCAAAAAATCCTGATGGAGACCTTAACGCCTTCTGCGATTTCCGAGCCGGAACCGGCGGCGACGAGGCGGCGCTGTTCGCGGGAGATCTGTTCTGTATGTACAGCCGTTTTGCCGAAGTCCGCCGCTGGCGGGTAGAAATCATGAg                <  2:526222/150‑1 (MQ=255)

                                                                                                                                 ccAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCa           <  2:125559/151‑1 (MQ=255)

                                                                                                                                   aaaaGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCCGc         >  1:73770/1‑151 (MQ=255)

                                                                                                                                     aaGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGGAGAAATCATGGGCGCCAGc         >  2:2601/1‑149 (MQ=255)

                                                                                                                                        aTCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAggg    <  2:316330/151‑1 (MQ=255)

                                                                                                                                           cTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGCggggg  >  1:561367/1‑148 (MQ=255)

CAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTG  >  NC_000913/1265186‑1265474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A