Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I2 R1 257 49.9 1554294 99.0% 1538751 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,069,351 T→C T153A (ACC→GCC)  mscS ← mechanosensitive channel protein, small conductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,069,3510TC100.0% 137.0 / NA 39T153A (ACC→GCC) mscSmechanosensitive channel protein, small conductance
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/16);  total (23/16)

CCTGATCGATATCGGAATCATACGCCACGCCAATAATAAATTCGTTACGGCGAACTGGCTCGCGGGAGAAGTTAATAATATTTCCGGCAATAATTTTACCGTTCGGAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGTGGAGAAAATCTGCACACTCAGCACAGTACCGGCTACGCCGCCCAGGTCAACATATTCTCCGGCACGGAACGGGCGGAACATGACAAGTAACACGCCAGCGGCCAGGTTAGACAGTGACCCCTGCAAAGCCAGACCAACAGCTAAGC  >  NC_000913/3069207‑3069497
                                                                                                                                                |                                                                                                                                                  
ccTGATCGATATCGGAATCATACGCCACGCCAATAATAAATTCGTTACGGCGAACTGGCTCGCGGGAGAAGTTAATAATATTTCCGGCAATAATTTTACCGTTCGGAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGCGgaga                                                                                                                                               >  2:699606/1‑150 (MQ=255)
ccTGATCGATATCGGAATCATACGCCACGCCAATAATAAATTCGTTACGGCGAACTGGCTCGCGGGAGAAGTTAATAATATTTCCGGCAATAATTTTACCGTTCGGAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGCGgaga                                                                                                                                               >  1:54150/1‑150 (MQ=255)
                aTCATACGCCACGCCAATAATAAATTCGTTACGGCGAACTGGCTCGCGGGAGAAGTTAATAATATTTCCGGCAATAATTTTACCGTTCGGAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGc                                                                                                                              <  2:146871/151‑1 (MQ=255)
                aTCATACGCCACGCCAATAATAAATTCGTTACGGCGAACTGGCTCGCGGGAGAAGTTAATAATATTTCCGGCAATAATTTTACCGTTCGGAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGc                                                                                                                              <  1:415055/151‑1 (MQ=255)
                  cATACGCCACGCCAATAATAAATTCGTTACGGCGAACTGGCTCGCGGGAGAAGTTAATAATATTTCCGGCAATAATTTTACCGTTCGGAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGca                                                                                                                             <  1:518458/150‑1 (MQ=255)
                   aTACGCCACGCCAATAATAAATTCGTTACGGCGAACTGGCTCGCGGGAGAAGTTAATAATATTTCCGGCAATAATTTTACCGTTCGGAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGcac                                                                                                                            >  2:658091/1‑150 (MQ=255)
                       gccacgccaATAATAAATTCGTTACGGCGAACTGGCTCGCGGGAGAAGTTAATAATATTTCCGGCAATAATTTTACCGTTCGGAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGCACAGTAc                                                                                                                       <  1:511642/151‑1 (MQ=255)
                         cacgccaATAATAAATTCGTTACGGCGAACTGGCTCGCGGGAGAAGTTAATAATATTTCCGGCAATAATTTTACCGTTCGGAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACCCTCAGCACAGTACCg                                                                                                                     <  1:635387/151‑1 (MQ=255)
                          acgccaATAATAAATTCGTTACGGCGAACTGGCTCGCGGGAGAAGTTAATAATATTTCCGGCAATAATTTTACCGTTCGGAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGCACAGTACCgg                                                                                                                    >  1:766863/1‑151 (MQ=255)
                          acgccaATAATAAATTCGTTACGGCGAACTGGCTCGCGGGAGAAGTTAATAATATTTCCGGCAATAATTTTACCGTTCGGAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGCACAGTACCgg                                                                                                                    >  2:367070/1‑151 (MQ=255)
                                    taaATTCGTTACGGCGAACTGGCTCGCGGGAGAAGTTAATAATATTTCCGGCAATAATTTTACCGTTCGGAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGCACAGTACCGGCTACgccgcc                                                                                                          >  1:321922/1‑151 (MQ=255)
                                      aaTTCGTTACGGCGAACTGGCTCGCGGGAGAAGTTAATAATATTTCCGGCAATAATTTTACCGTTCGGAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGCACAGTACCGGCTACGCCGccc                                                                                                         >  2:63965/1‑150 (MQ=255)
                                         tCGTTACGGCGAAATGGCTCGCGGGAGAAGTTAATAATTTTTCCGGCAATAATTTTACCGTGCGGAATAACGATAATTTTACCGTCGTCAGGACTCATGGTGGCGGAGAAAATGTGCACACTCAGCACAGTACAGGCAAGGCCGCCCAGGt                                                                                                     >  1:426175/1‑151 (MQ=255)
                                           gTTACGGCGAACTGGCTCGCGGGAGAAGTTAATAATATTTCCGGCAATAATTTTACCGTTCGGAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGCACAGTACCGGCTACGCCGCCCAGGTCa                                                                                                   <  1:699532/151‑1 (MQ=255)
                                            ttACGGCGAACTGGCTCGCGGGAGAAGTTAATAATATTTCCGGCAATAATTTTACCGTTCGGAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGCACAGTACCGGCTACGCCGCCCAGGTCa                                                                                                   >  1:632019/1‑150 (MQ=255)
                                             tACGGCGAACTGGCTCGCGGGAGAAGTTAATAATATTTCCGGCAATAATTTTACCGTTCGGAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGCACAGTACCGGCTACGCCGCCCAGGTCaa                                                                                                  <  1:378572/150‑1 (MQ=255)
                                             tACGGCGAACTGGCTCGCGGGAGAAGTTAATAATATTTCCGGCAATAATTTTACCGTTCGGAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGCACAGTACCGGCTACGCCGCCCAGGTCAAc                                                                                                 >  1:52635/1‑151 (MQ=255)
                                                     aCTGGCTCGCGGGAGAAGTTAATAATATTTCCGGCAATAATTTTACCGTTCGGAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGCACAGTACCGGCTACGCCGCCCAGGTCAACATATtctc                                                                                         <  1:620065/151‑1 (MQ=255)
                                                        ggCTCGCGGGAGAAGTTAATAATATTTCCGGCAATAATTTTACCGTTCGGAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGCACAGTACCGGCTACGCCGCCCAGGTCAACATATTCTCCgg                                                                                      <  2:310551/151‑1 (MQ=255)
                                                                      gTTAATAATATTTCCGGCAATAATTTTACCGTTCGGAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGCACAGTACCGGCTACGCCGCCCAGGTCAACATATTCTCCGGCACGGAACGGGCgg                                                                        <  2:52666/151‑1 (MQ=255)
                                                                          ataataTTTCCGGCAATAATTTTACCGTTCGGAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGCACAGTACCGGCTACGCCGCCCAGGTCAACATATTCTCCGGCACGGAACGGGCGGAAc                                                                     >  2:679217/1‑150 (MQ=255)
                                                                            aataTTTCCGTCAATAATTTTACCGTTCGGAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGCACAGTACCGGCTACGCCGCCCAGGTCAACATATTCTCCGGCACGGAACGGGCGGAACATg                                                                  >  1:81342/1‑151 (MQ=255)
                                                                             atatTTCCGGCAATAATTTTCCCGCTCGGAATAACGATAATTTTACCGCCTGCAGTACGCATGGTCGCGGAGAAAATCTGCCCGCTCAGCACAGTTCCGCCTACGCCGCCCAGGTCAATATATGCTCCGGCACGGAACGGGCGTAACATg                                                                  <  1:577292/150‑1 (MQ=255)
                                                                                tttCCGGCAATAATTTTACCGTTCGGAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGCACAGTACCGGCTACGCCGCCCAGGTCAACATATTCTCCGGCACGGAACGGGCGGAACATGACa                                                               >  1:72575/1‑150 (MQ=255)
                                                                                tttCCGGCAATAATTTTACCGTTCGGAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGCACAGTACCGGCTACGCCGCCCAGGTCAACATATTCTCCGGCACGGAACGGGCGGAACATGACa                                                               >  1:292504/1‑150 (MQ=255)
                                                                                       cAATAATTTTACCGTTCGGAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGCACAGTACCGGCTACGCCGCCCAGGTCAACATATTCTCCGGCACGGAACGGGCGGAACATGACAAGTAacac                                                       <  2:60013/151‑1 (MQ=255)
                                                                                         ataatTTTACCGTTCGGAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGCACAGTACCGGCTACGCCGCCCAGGTCAACATATTCTCCGGCACGGAACGGGCGGAACATGACAAGTAACACGc                                                     <  1:480382/151‑1 (MQ=255)
                                                                                             ttttACCGTTCGGAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGCACAGTACCGGCTACGCCGCCCAGGTCAACATATTCTCCGGCACGGAACGGGCGGAACATGACAAGTAACACGCCAGc                                                 >  1:709396/1‑151 (MQ=255)
                                                                                              tttACCGTTCGGAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGCACAGTACCGGCTACGCCGCCCAGGTCAACATATTCTCCGGCACGGAACGGGCGGAACATGACAAGTAACACGCCAGc                                                 <  1:90530/150‑1 (MQ=255)
                                                                                                   cGTTCGGAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGCACAGTACCGGCTACGCCGCCCAGGTCAACATATTCTCCGGCACGGAACGGGCGGAACATGACAAGTAACACGCCAGCGGcc                                             >  1:284333/1‑149 (MQ=255)
                                                                                                         gAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGCACAGTACCGGCTACGCCGCCCAGGTCAACATATTCTCCGGCACGGAACGGGCGGAACATGACAAGTAACACGCCAGCGGCCAGGTTAGa                                     <  2:24659/151‑1 (MQ=255)
                                                                                                            tAACGATAATTTTACCGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGCACAGTACCGGCTACGCCGCCCAGGTCAACATATTCTCCGGCACGGAACGGGCGGAACATGACAAGTAACACGCCAGCGGCCAGGTTAGAc                                    >  1:332613/1‑149 (MQ=255)
                                                                                                              aCGATAATTTTACCGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGCACAGTACCGGCTACGCCGCCCAGGTCAACATATTCTCCGGCACGGAACGGGCGGAACATGACAAGTAACACGCCAGCGGCCAGGTTAGACAGTg                                >  2:769090/1‑151 (MQ=255)
                                                                                                                   aaTTTTACCGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGCACAGTACCGGCTACGCCGCCCAGGTCAACATATTCTCCGGCACGGAACGGGCGGAACATGACAAGTAACACGCCAGCGGCCAGGTTAGACAGTGAcccc                           >  1:450663/1‑151 (MQ=255)
                                                                                                                          ccGTCTGCAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGCACAGTACCGGCTACGCCGCCCAGGTCAACATATTCTCCGGCACGGAACGGGCGGAACATGACAAGTAACACGCCAGCGGCCAGGTTAGACAGTGACCCCTGCAAAg                    <  2:55629/151‑1 (MQ=255)
                                                                                                                                 cAGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGCACAGTACCGGCTACGCCGCCCAGGTCAACATATTCTCCGGCACGGAACGGGCGGAACATGACAAGTAACACGCCAGCGGCCAGGTTAGACAGTGACCCCTGCAAAGCCAGAcc             >  1:53496/1‑151 (MQ=255)
                                                                                                                                  aGTACGCATGGTGGCGGAGAAAATCTGCACACTCAGCACAGTACCGGCTACGCCGCCCAGGTCAACATATTCTCCGGCACGGAACGGGCGGAACATGACAAGTAACACGCCAGCGGCCAGGTTAGACAGTGACCCCTGCAAAGCCAGAcc             >  2:24735/1‑150 (MQ=255)
                                                                                                                                       gCATGGTGGCGGAGAAAATCTGCACACTCAGCACAGTACCGGCTACGCCGCCCAGGTCAACATATTCTCCGGCACGGAACGGGCGGAACATGACAAGTAACACGCCAGCGGCCAGGTTAGACAGTGACCCCTGCAAAGCCAGACCAACAGc       >  1:107788/1‑151 (MQ=255)
                                                                                                                                        cATGGTGGCGGAGAAAATCTGCACACTCAGCACAGTACCGGCTACGCCGCCCAGGTCAACATATTCTCCGGCACGGAACGGGCGGAACATGACAAGTAACACGCCAGCGGCCAGGTTAGACAGTGACCCCTGCAAAGCCAGACCAACAGCt      >  2:699401/1‑151 (MQ=255)
                                                                                                                                            gtggcggAGAAAATCTGCACACTCAGCACAGTACCGGCTACGCCGCCCAGGTCAACATATTCTCCGGCACGGAACGGGCGGAACATGACAAGTAACACGCCAGCGGCCAGGTTAGACAGTGACCCCTGCAAAGCCAGACCAACAGCTAAGc  <  2:214559/151‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CCTGATCGATATCGGAATCATACGCCACGCCAATAATAAATTCGTTACGGCGAACTGGCTCGCGGGAGAAGTTAATAATATTTCCGGCAATAATTTTACCGTTCGGAATAACGATAATTTTACCGTCTGCAGTACGCATGGTGGTGGAGAAAATCTGCACACTCAGCACAGTACCGGCTACGCCGCCCAGGTCAACATATTCTCCGGCACGGAACGGGCGGAACATGACAAGTAACACGCCAGCGGCCAGGTTAGACAGTGACCCCTGCAAAGCCAGACCAACAGCTAAGC  >  NC_000913/3069207‑3069497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A