Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,083,334	T→C	Y155C (TAT→TGT)	speB ←	agmatinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,083,334	0	T	C	100.0%	175.6 / NA	48	Y155C (TAT→TGT)	speB	agmatinase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/26); total (22/26)

GGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCG  >  NC_000913/3083191‑3083474

ggCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATc                                                                                                                                       <  2:431278/151‑1 (MQ=255)

      cGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGtgtg                                                                                                                                 >  2:651605/1‑151 (MQ=255)

         ccAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTggg                                                                                                                               <  1:233934/150‑1 (MQ=255)

         ccAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGc                                                                                                                              <  2:560363/151‑1 (MQ=255)

               cGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCaaa                                                                                                                        <  2:629596/151‑1 (MQ=255)

               cGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCaaa                                                                                                                        <  1:366298/151‑1 (MQ=255)

                   aaaGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAgtgt                                                                                                                    >  1:330456/1‑151 (MQ=255)

                   aaaGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAgtgt                                                                                                                    >  2:327305/1‑151 (MQ=255)

                    aaGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAgtgt                                                                                                                    >  2:193081/1‑150 (MQ=255)

                                  aTCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTg                                                                                                     <  1:801546/151‑1 (MQ=255)

                                  aTCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTg                                                                                                     <  1:480853/151‑1 (MQ=255)

                                   tCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGc                                                                                                    >  2:289033/1‑151 (MQ=255)

                                        cTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGaa                                                                                                >  1:210364/1‑150 (MQ=255)

                                          cGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATg                                                                                             <  1:654093/151‑1 (MQ=255)

                                              aCGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTc                                                                                         <  2:62864/151‑1 (MQ=255)

                                                 aaTACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGc                                                                                       >  1:825541/1‑150 (MQ=255)

                                                     ccAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGa                                                                                   <  2:664192/150‑1 (MQ=255)

                                                       aaTCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGCTCGCACAGGGATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGa                                                                                   >  2:56258/1‑148 (MQ=255)

                                                             cACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACgcagc                                                                          <  1:697438/151‑1 (MQ=255)

                                                               cGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGcagcag                                                                        <  2:521492/151‑1 (MQ=255)

                                                               cGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGcagcag                                                                        <  2:830634/151‑1 (MQ=255)

                                                                ggAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCagcagc                                                                       <  2:548641/151‑1 (MQ=255)

                                                                ggAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCagcagc                                                                       >  2:228459/1‑151 (MQ=255)

                                                                 gAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcg                                                                      <  2:438265/151‑1 (MQ=255)

                                                                     gactccgttCGAACACACCTTCTTTCGGCGAGCTATAGAACATAGTGCCGTGGTCAAACTCCCGACCGTTCGCACAGCTATCTGTGTACGCGTCAAATTGCCCCAGCGCCATTTAGCCGAAATACTTCGCATGATCCCGCAgcagcggca                                                                   <  1:523262/142‑1 (MQ=255)

                                                                       ttCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcggcagcg                                                                >  1:218636/1‑151 (MQ=255)

                                                                         cGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTa                                                              <  1:332647/151‑1 (MQ=255)

                                                                              cGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACaa                                                          >  1:43189/1‑150 (MQ=255)

                                                                                   aGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGt                                                    >  1:560553/1‑151 (MQ=255)

                                                                                    gACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTc                                                   >  2:175533/1‑151 (MQ=255)

                                                                                      ccTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTcac                                                 >  1:800716/1‑151 (MQ=255)

                                                                                      ccTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTcac                                                 <  2:111354/151‑1 (MQ=255)

                                                                                       cttcttTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTcacc                                                >  1:39907/1‑151 (MQ=255)

                                                                                          cttTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCaccacc                                             >  1:667529/1‑151 (MQ=255)

                                                                                              cGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGaa                                          <  1:472811/150‑1 (MQ=255)

                                                                                                 cgcgGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAagag                                      <  1:610231/151‑1 (MQ=255)

                                                                                                 cgcgGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAag                                        >  2:854365/1‑149 (MQ=255)

                                                                                                           aaCATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCt                            <  2:359550/151‑1 (MQ=255)

                                                                                                           aaCATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCGTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCt                            <  1:774307/151‑1 (MQ=255)

                                                                                                            aCATAGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCtt                           >  2:46502/1‑151 (MQ=255)

                                                                                                                aGTGCCGTGGTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTAcc                        <  1:851240/150‑1 (MQ=255)

                                                                                                                        ggTCAAATTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGcagccag               <  1:432606/151‑1 (MQ=255)

                                                                                                                            aaaTTCACAACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCagcagc           >  1:496350/1‑151 (MQ=255)

                                                                                                                                  acaACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCg     <  1:505022/151‑1 (MQ=255)

                                                                                                                                   caACCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCgg    >  1:361002/1‑151 (MQ=255)

                                                                                                                                    aaCCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGc   <  2:353299/151‑1 (MQ=255)

                                                                                                                                     aCCGTTCGCACAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCg  >  1:22538/1‑151 (MQ=255)

                                                                                                                                     aCCGCTCGCACAGGTATCGGTGTGGGCGTCAAAGTGCACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGc   >  1:184681/1‑150 (MQ=255)

GGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCG  >  NC_000913/3083191‑3083474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A