Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F50 I1 R1
|
300 |
54.7 |
1718999 |
98.4% |
1691495 |
149.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,108,362 |
G→A |
A265V (GCG→GTG) |
speC ← |
ornithine decarboxylase, constitutive |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,108,362 | 0 | G | A | 97.8%
| 146.5
/ ‑4.4
| 47 | A265V (GCG→GTG) | speC | ornithine decarboxylase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (23/22); total (24/23) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.56e-01 |
GACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGCCGTGATGATTCGACTTA > NC_000913/3108217‑3108508
|
gACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACg < 2:164751/151‑1 (MQ=255)
gACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACg > 1:66806/1‑151 (MQ=255)
cACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTcgc < 2:592660/151‑1 (MQ=255)
cgcgGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGGCGGCTTTTTCTGGCACAACGTcgcg > 1:663034/1‑150 (MQ=255)
cgGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAAt < 2:48889/151‑1 (MQ=255)
cAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTg > 1:703079/1‑151 (MQ=255)
aaaCAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTgg < 2:69276/151‑1 (MQ=255)
aaCAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGgc > 2:712003/1‑151 (MQ=255)
aaCAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGgc > 2:321602/1‑151 (MQ=255)
aCAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGgcg > 1:639732/1‑151 (MQ=255)
cAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACATCGCGAATTTGCTGgcgc < 1:637581/151‑1 (MQ=255)
gAATGTAATCACACAGATGCCCAACGGGATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGCCGGCTTTTTCTGGCACCATGCCGCGAATTTGCTGgcg > 1:450457/1‑148 (MQ=255)
aTGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGAt < 2:188837/151‑1 (MQ=255)
gTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATa > 1:454774/1‑150 (MQ=255)
aTCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCACGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATActct > 1:108579/1‑151 (MQ=255)
cacacaGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTc > 2:415816/1‑151 (MQ=255)
cacacaGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTc < 1:538905/151‑1 (MQ=255)
acaGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCAtt < 2:79743/151‑1 (MQ=255)
ccAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAg > 2:607577/1‑150 (MQ=255)
cAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATGTGCTGGCGCAGATACTCTTCATTAAAACAGTg < 1:426289/151‑1 (MQ=255)
aCGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTgcg > 2:583161/1‑151 (MQ=255)
aTCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATc > 2:1612/1‑150 (MQ=255)
gATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATa < 2:433342/151‑1 (MQ=255)
ccTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGcc < 2:57259/151‑1 (MQ=255)
gCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGaaa > 1:193144/1‑150 (MQ=255)
ataGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGGCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAAc > 1:404227/1‑151 (MQ=255)
aGACAGTGCCGTCATGGGTTCCCAGCTAAATAAGCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAAATCCTCTTCATTAAAACAGTGCGCATAAATACCGCCAATGAAACCAAACgg < 1:216442/151‑1 (MQ=255)
aCAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATCCTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACgg < 2:13853/149‑1 (MQ=255)
aCAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGt > 2:586362/1‑151 (MQ=255)
gTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACACCGTCGCGAATTTGCGGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGaa < 2:119420/151‑1 (MQ=255)
ggTTCCCAGCTAAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATAAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTc < 1:415784/150‑1 (MQ=255)
cccAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGAt < 2:574656/151‑1 (MQ=255)
cccAGCTAAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGAt < 2:435942/151‑1 (MQ=255)
ccAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGAt > 1:457348/1‑150 (MQ=255)
ataatCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCg > 2:61735/1‑151 (MQ=255)
cAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGccccc > 1:410442/1‑151 (MQ=255)
aaaCGGGCGCGGCAGGGCGGTTTTTTCTGGCACAACGTCGCGAATTTGCGGGCGCGGATACCCTTCATTAAAACAGTCCGCATAAACACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCt < 1:261282/147‑1 (MQ=255)
aCGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAAt > 1:795027/1‑151 (MQ=255)
gggCGCGGCGGGCGGTCTTTTTCTGGCACACGGCCGGAAATTTCCGGACGCAAATCCTCTCCTTAAAAAAAGCCCGCATAAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGCCCGGCGTCGCCCCCGCCTGAATc < 1:673488/150‑1 (MQ=255)
gcgGCAGGTCGTTTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATCCTCTTCATAAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAgcg < 2:675112/150‑1 (MQ=255)
ggCAGGTCGGTTTTTCCTGACCCCCCGGCGCGAATTTGCGGGCGTAGATACTCTTCATAAAACCAGAGCGCAAAAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAgcgcg < 2:467982/150‑1 (MQ=255)
gCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGcc > 2:298831/1‑151 (MQ=255)
aGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGACACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGcc > 1:505349/1‑149 (MQ=255)
cGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGCCGtgatg > 1:716110/1‑151 (MQ=255)
tGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGCCGTGATGATTCGACtt > 2:106873/1‑150 (MQ=255)
ggCACACCGGCGCGAATTTGCTGGCGCAAATCCTCTTCATAAAAACAGTGCGCATAAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGCCGTGATGATTCGACt < 2:541338/148‑1 (MQ=255)
ggCACAACGTCGCGAATTGGCGGGCGCAGATACTCTTCATAAAAACAGTGCGCATAAATCCCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGCCGTGATGATTCGACTTa < 1:57958/150‑1 (MQ=255)
|
GACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGCCGTGATGATTCGACTTA > NC_000913/3108217‑3108508
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
