Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,108,362	G→A	A265V (GCG→GTG)	speC ←	ornithine decarboxylase, constitutive

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,108,362	0	G	A	100.0%	141.1 / NA	39	A265V (GCG→GTG)	speC	ornithine decarboxylase, constitutive
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/17); total (22/17)

CGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGCCGTGATGATTCGAC  >  NC_000913/3108216‑3108505

cGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAAc                                                                                                                                             >  2:515196/1‑151 (MQ=255)

  aCCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGt                                                                                                                                           <  2:743536/151‑1 (MQ=255)

   cccACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTc                                                                                                                                          <  1:185719/151‑1 (MQ=255)

         cgGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAAt                                                                                                                                    >  1:349115/1‑151 (MQ=255)

         cgGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAAt                                                                                                                                    <  1:408950/151‑1 (MQ=255)

                aaaCAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTgg                                                                                                                             >  2:761460/1‑151 (MQ=255)

                    aGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGTGAATTTGCTGGCGCa                                                                                                                         <  2:11523/151‑1 (MQ=255)

                      aaTGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGgcgc                                                                                                                          >  1:241947/1‑148 (MQ=255)

                       aTGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGAt                                                                                                                      <  1:370059/151‑1 (MQ=255)

                       aTGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTAAAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGAt                                                                                                                      <  2:319878/151‑1 (MQ=255)

                         gTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATa                                                                                                                     <  1:593906/150‑1 (MQ=255)

                           aaTCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATActc                                                                                                                  >  2:236197/1‑151 (MQ=255)

                                 acaGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCAtt                                                                                                            >  2:506530/1‑151 (MQ=255)

                                  caGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCAtt                                                                                                            >  1:118040/1‑150 (MQ=255)

                                    gATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTaaa                                                                                                         <  1:699262/151‑1 (MQ=255)

                                    gATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTaa                                                                                                          >  1:247934/1‑150 (MQ=255)

                                       gCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAAc                                                                                                       >  2:105133/1‑150 (MQ=255)

                                       gCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAAc                                                                                                       >  2:615685/1‑150 (MQ=255)

                                        cccAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAg                                                                                                     <  2:274237/151‑1 (MQ=255)

                                                   cGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAAt                                                                                          <  1:238135/151‑1 (MQ=255)

                                                   cGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAAt                                                                                          <  2:513445/151‑1 (MQ=255)

                                                     aTCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATAc                                                                                        <  2:359646/151‑1 (MQ=255)

                                                            gACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCa                                                                                   <  2:125836/149‑1 (MQ=255)

                                                                 gCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGaaa                                                                             >  1:262638/1‑150 (MQ=255)

                                                                    ttATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCa                                                                          >  2:520903/1‑150 (MQ=255)

                                                                     tataGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCaa                                                                         <  2:719650/150‑1 (MQ=255)

                                                                      ataGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAAc                                                                       >  1:438235/1‑151 (MQ=255)

                                                                               gCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGt                                                              >  2:286671/1‑151 (MQ=255)

                                                                                            cccAGCTGAATAACCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATCCTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGAt                                                 <  2:384467/151‑1 (MQ=255)

                                                                                                       aatCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACACCGTCGCGAATTTGCTGGCGCAGATACTTTTCTTTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGt                                       <  2:579623/150‑1 (MQ=255)

                                                                                                        atCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCg                                     >  2:765351/1‑151 (MQ=255)

                                                                                                              aGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGccccc                                >  2:640711/1‑150 (MQ=255)

                                                                                                                 cGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGcc                             >  1:776024/1‑150 (MQ=255)

                                                                                                                 cGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCt                            >  2:66909/1‑151 (MQ=255)

                                                                                                                    tACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGa                          >  2:545881/1‑150 (MQ=255)

                                                                                                                        ggCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCGTCCAGATAGACCGGCGTCGCCCCCGCCTGAAt                        >  1:27350/1‑148 (MQ=255)

                                                                                                                                         ttttCTGGCACAACGTCGCGAATTTGCGGGCGCAGATACTCTTCATAAAAACAGTGCGCATAAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGCCGTGATGAtt      <  2:454850/149‑1 (MQ=255)

                                                                                                                                          tttCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGCCGTGATGATTCg    >  2:352961/1‑150 (MQ=255)

                                                                                                                                           ttCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGCCGTGATGATTCGAc  >  2:89107/1‑151 (MQ=255)

CGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGCCGTGATGATTCGAC  >  NC_000913/3108216‑3108505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A