Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I3 R1	283	58.3	1819865	98.8%	1798026	150.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,108,362	G→A	A265V (GCG→GTG)	speC ←	ornithine decarboxylase, constitutive

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,108,362	0	G	A	100.0%	165.7 / NA	48	A265V (GCG→GTG)	speC	ornithine decarboxylase, constitutive
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/26); total (22/26)

CGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGCCGTGATGATTCG  >  NC_000913/3108216‑3108503

cGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAAc                                                                                                                                           >  2:12149/1‑151 (MQ=255)

 gACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAAc                                                                                                                                           >  2:866007/1‑150 (MQ=255)

 gACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACg                                                                                                                                          >  2:709471/1‑151 (MQ=255)

    ccACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTcg                                                                                                                                       <  2:638438/151‑1 (MQ=255)

    ccACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTc                                                                                                                                        <  1:226342/150‑1 (MQ=255)

       cgcgGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTcgcg                                                                                                                                     >  1:416737/1‑150 (MQ=255)

       cgcgGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGAACAACGTcgcg                                                                                                                                     >  1:549326/1‑150 (MQ=255)

         cgGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAAt                                                                                                                                  >  1:894650/1‑151 (MQ=255)

          ggAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAAtt                                                                                                                                 <  2:117932/151‑1 (MQ=255)

           gAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAAttt                                                                                                                                <  2:478461/151‑1 (MQ=255)

           gAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAAttt                                                                                                                                <  2:326954/151‑1 (MQ=255)

           gAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAAttt                                                                                                                                <  1:537077/151‑1 (MQ=255)

           gAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAAttt                                                                                                                                <  2:582124/151‑1 (MQ=255)

            aaTCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTg                                                                                                                               <  2:465410/151‑1 (MQ=255)

                           aaTCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATAct                                                                                                                 <  2:461763/150‑1 (MQ=255)

                                 acaGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCAtt                                                                                                          >  1:63755/1‑151 (MQ=255)

                                 acaGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCAtt                                                                                                          <  1:644574/151‑1 (MQ=255)

                                   aGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTaa                                                                                                        <  2:268704/151‑1 (MQ=255)

                                    gATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTaaa                                                                                                       <  2:401797/151‑1 (MQ=255)

                                     aTGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTaaaa                                                                                                      <  1:297566/151‑1 (MQ=255)

                                         ccAACGGTATCGATCGCCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGt                                                                                                  <  2:653041/151‑1 (MQ=255)

                                         ccAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAg                                                                                                   >  1:578094/1‑150 (MQ=255)

                                           aaCGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTgc                                                                                                <  1:160267/151‑1 (MQ=255)

                                                tATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATc                                                                                           >  1:593933/1‑151 (MQ=255)

                                                   cGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCaa                                                                                         <  2:200325/150‑1 (MQ=255)

                                                     aTCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATAc                                                                                      >  1:114153/1‑151 (MQ=255)

                                                      tCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATAcc                                                                                     >  2:818615/1‑151 (MQ=255)

                                                       cACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATAcc                                                                                     <  1:709520/150‑1 (MQ=255)

                                                                          aCAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCAAATTTGCTGGCGAAGATCCTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAAc                                                                     <  2:593884/147‑1 (MQ=255)

                                                                          aCAGTGCCGTCATAGGTTCCCAGCTAAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATCCTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACgg                                                                   <  1:112187/149‑1 (MQ=255)

                                                                             gTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGc                                                              >  2:524236/1‑151 (MQ=255)

                                                                               gCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGt                                                            >  1:805628/1‑151 (MQ=255)

                                                                                   tCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGa                                                          >  1:908604/1‑149 (MQ=255)

                                                                                    cATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGTTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAg                                                        <  2:228469/150‑1 (MQ=255)

                                                                                    cATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAg                                                        <  1:159242/150‑1 (MQ=255)

                                                                                     aTAGGTCCCCAGCTGAATAACCGCCAGGCGATACGGGCGCGGCAGGTCGGTTTTTTCTGGCAAAACGTCGCAAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGa                                                          <  1:15804/147‑1 (MQ=255)

                                                                                       aGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTc                                                    >  1:890833/1‑151 (MQ=255)

                                                                                                       aatCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTc                                    <  1:623435/151‑1 (MQ=255)

                                                                                                        atCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTc                                    >  2:751438/1‑150 (MQ=255)

                                                                                                           gCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGccc                                >  1:347251/1‑151 (MQ=255)

                                                                                                              aGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCg                             >  1:265956/1‑151 (MQ=255)

                                                                                                                gCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGcc                           >  1:825767/1‑151 (MQ=255)

                                                                                                                    tACGGGCGCGGCAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGa                        >  1:625192/1‑150 (MQ=255)

                                                                                                                       gggCGCGGCAGGTCGGTTTTTTCTGGCACACCGCCGCAATTTTGCGGGCGCAGATCCTCTTCATAAAAACAGTGCGCATAAATCCCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCa                    <  2:894578/151‑1 (MQ=255)

                                                                                                                          cgcgGCAGGTCGGCTTTTTCTGGCACACCGTCGCAATTTTGCGGGCGCAAATACTCTTCATAAAAACAGGGCGCATCAAACCCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAgc                  <  1:268684/150‑1 (MQ=255)

                                                                                                                               cAGGTCGGCTTTTTCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGCCg            >  1:864810/1‑151 (MQ=255)

                                                                                                                                     ggCTTTTTTCGGCACACCGTCGAGATTTTGCTGGCACAGCTCCTCTTCATAAAAACAGTCCGAACCAAACCCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGCCGtga         <  2:276982/148‑1 (MQ=255)

                                                                                                                                         ttttCTGGCACAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGCCGTGATGATTCg  >  1:437613/1‑151 (MQ=255)

CGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTGGCGCAGATACTCTTCATTAAAACAGTGCGCATCAATACCGCCAATGAAACCAAACGGGTTGCGTGAAGCTTCCAGATAGACCGGCGTCGCCCCCGCCTGAATCAGCGCGCCGTGATGATTCG  >  NC_000913/3108216‑3108503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A