Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,231,746	A→G	T28A (ACC→GCC)	fadH →	2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,231,746	0	A	G	100.0%	127.8 / NA	37	T28A (ACC→GCC)	fadH	2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/19); total (18/19)

CGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCG  >  NC_000913/3231601‑3231881

cGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCggg                                                                                                                                    <  2:25823/151‑1 (MQ=255)

cGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCggg                                                                                                                                    <  1:688868/151‑1 (MQ=255)

    gACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTgg                                                                                                                                >  2:765339/1‑151 (MQ=255)

       ggCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTggagg                                                                                                                             >  2:206825/1‑151 (MQ=255)

           aaaTTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAAt                                                                                                                          >  2:11681/1‑150 (MQ=255)

           aaaTTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAAt                                                                                                                          <  1:83422/150‑1 (MQ=255)

             aTTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATAcc                                                                                                                       <  2:737402/151‑1 (MQ=255)

                 gAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGa                                                                                                                   <  1:490942/151‑1 (MQ=255)

                        tCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGcc                                                                                                            >  2:456695/1‑151 (MQ=255)

                           gACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCg                                                                                                           >  2:241491/1‑149 (MQ=255)

                           gACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAg                                                                                                         <  1:408936/151‑1 (MQ=255)

                           gACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAg                                                                                                         <  2:480122/151‑1 (MQ=255)

                                        ttattTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGt                                                                                            >  1:77188/1‑151 (MQ=255)

                                         tattTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGtt                                                                                           >  1:132985/1‑151 (MQ=255)

                                          attTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGtt                                                                                           <  1:635334/150‑1 (MQ=255)

                                                      ggACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAAcgc                                                                              >  1:713518/1‑151 (MQ=255)

                                                       gACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAAcgc                                                                              >  1:645451/1‑150 (MQ=255)

                                                           aTTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCg                                                                         >  1:233059/1‑151 (MQ=255)

                                                                    gCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGt                                                                <  1:241044/151‑1 (MQ=255)

                                                                        cccGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGgcg                                                            >  2:448760/1‑151 (MQ=255)

                                                                          cGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCt                                                          <  1:694592/151‑1 (MQ=255)

                                                                            tCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGgcgc                                                           >  1:41112/1‑148 (MQ=255)

                                                                            tCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTg                                                         >  2:607263/1‑150 (MQ=255)

                                                                              gCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGAtt                                                      <  2:395570/151‑1 (MQ=255)

                                                                              gCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGAtt                                                      <  1:411793/151‑1 (MQ=255)

                                                                              gCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGAtt                                                      >  2:441592/1‑151 (MQ=255)

                                                                                 gTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTg                                                     <  2:324954/149‑1 (MQ=255)

                                                                                     gCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAgcg                                               >  1:527552/1‑151 (MQ=255)

                                                                                            tGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCAt                                        >  1:712413/1‑151 (MQ=255)

                                                                                              gATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACTGCGTGGCGCTGATTGTCAGCGGCGGCATTg                                      <  1:688377/151‑1 (MQ=255)

                                                                                                         tACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGAttt                            >  2:2104/1‑150 (MQ=255)

                                                                                                          aCCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTa                           <  2:592236/150‑1 (MQ=255)

                                                                                                           ccACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAAc                         <  2:342241/151‑1 (MQ=255)

                                                                                                                     aaaCCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGc               >  1:487340/1‑151 (MQ=255)

                                                                                                                      aaCCGCGTGTTGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCa              <  1:561596/151‑1 (MQ=255)

                                                                                                                                tGATGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTg     <  2:406030/150‑1 (MQ=255)

                                                                                                                                  aTGGGCTCAATGCACGCCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGgcg  <  1:590269/151‑1 (MQ=255)

CGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTCAATGCACACCGGGCTGGAGGAATACCCGGACGGTGCCGAGCGGCTGGCAGCGTTTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCG  >  NC_000913/3231601‑3231881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A