Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,286,334	T→C	L22P (CTT→CCT)	agaI →	galactosamine‑6‑phosphate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,286,334	0	T	C	100.0%	179.6 / NA	50	L22P (CTT→CCT)	agaI	galactosamine‑6‑phosphate isomerase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (30/20); total (30/20)

AAAAGCGCATCCGGTGCTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCTTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTATCTGGTAGAAAAAATCC  >  NC_000913/3286194‑3286480

aaaaGCGCATCCGGTGCTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGtt                                                                                                                                          >  1:56240/1‑151 (MQ=255)

      gcATCCGGTGCTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAAc                                                                                                                                    >  1:151574/1‑151 (MQ=255)

       cATCCGGTGCTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACt                                                                                                                                   >  1:676467/1‑151 (MQ=255)

               gCTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGc                                                                                                                            <  1:13213/150‑1 (MQ=255)

                    tAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTa                                                                                                                        >  1:257031/1‑149 (MQ=255)

                    tAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTa                                                                                                                        >  2:480634/1‑149 (MQ=255)

                          gCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACg                                                                                                                <  1:277675/151‑1 (MQ=255)

                               aCTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGcc                                                                                                            >  2:332316/1‑150 (MQ=255)

                                cTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGcc                                                                                                            >  2:162143/1‑149 (MQ=255)

                                  tttGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAg                                                                                                          <  2:473276/149‑1 (MQ=255)

                                  tttGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGc                                                                                                         <  1:21818/150‑1 (MQ=255)

                                    tgtgCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGa                                                                                                       >  1:651969/1‑150 (MQ=255)

                                     gtgCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAat                                                                                                     <  2:633949/151‑1 (MQ=255)

                                       gCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAatat                                                                                                   <  2:56271/151‑1 (MQ=255)

                                         tctctATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAAtattt                                                                                                 >  2:483962/1‑151 (MQ=255)

                                               ttgtttgtTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGc                                                                                           >  1:521923/1‑151 (MQ=255)

                                                tgtttgttCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGcc                                                                                          >  1:587351/1‑151 (MQ=255)

                                                    tgttCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTg                                                                                       >  1:395756/1‑150 (MQ=255)

                                                     gttCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGAt                                                                                     <  1:554109/151‑1 (MQ=255)

                                                          gCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGt                                                                                 >  2:693364/1‑150 (MQ=255)

                                                          gCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTa                                                                                <  2:573898/151‑1 (MQ=255)

                                                           cATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGt                                                                                 >  1:130278/1‑149 (MQ=255)

                                                            aTTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGc                                                                              >  2:348752/1‑151 (MQ=255)

                                                                ggCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAAc                                                                          <  2:477632/151‑1 (MQ=255)

                                                                    ttttGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGa                                                                       >  2:361470/1‑150 (MQ=255)

                                                                      ttGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAAt                                                                     <  2:567894/150‑1 (MQ=255)

                                                                       tGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGc                                                                   >  2:297490/1‑151 (MQ=255)

                                                                           aaTGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTg                                                               >  1:115619/1‑151 (MQ=255)

                                                                           aaTGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTg                                                               >  1:13085/1‑151 (MQ=255)

                                                                              ggAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGAtt                                                            <  2:729447/151‑1 (MQ=255)

                                                                                      gCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGc                                                    >  2:94752/1‑151 (MQ=255)

                                                                                        aCTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCg                                                   >  2:216498/1‑150 (MQ=255)

                                                                                         cTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGAc                                                 >  1:687665/1‑151 (MQ=255)

                                                                                           gCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCg                                               <  2:760136/151‑1 (MQ=255)

                                                                                           gCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCg                                               <  1:661234/151‑1 (MQ=255)

                                                                                                 ggtggtGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGcc                                          >  2:500800/1‑150 (MQ=255)

                                                                                                        ccTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCAtt                                  >  1:397313/1‑151 (MQ=255)

                                                                                                             ttACTTAAGGAATTTCATCCTGTGCACACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACt                               >  2:424088/1‑149 (MQ=255)

                                                                                                                  tAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTa                         <  1:711829/150‑1 (MQ=255)

                                                                                                                  tAAGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTa                         <  1:693290/150‑1 (MQ=255)

                                                                                                                    aGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATc                       >  1:503921/1‑150 (MQ=255)

                                                                                                                    aGGAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCa                      <  1:667019/151‑1 (MQ=255)

                                                                                                                     ggAATTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCAt                     <  2:2052/151‑1 (MQ=255)

                                                                                                                        aTTTCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTAt                  >  1:316489/1‑151 (MQ=255)

                                                                                                                         tttCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTATc                 >  1:310428/1‑151 (MQ=255)

                                                                                                                           tCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTATCt                <  2:299026/150‑1 (MQ=255)

                                                                                                                           tCATCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTATCt                <  2:257064/150‑1 (MQ=255)

                                                                                                                              tCCTGTGCAAACCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTATCTGGt             >  1:538048/1‑150 (MQ=255)

                                                                                                                                      aaaCCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTATCTGGTAGAAAAAAt    <  1:170848/151‑1 (MQ=255)

                                                                                                                                        aCCCCTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTATCTGGTAGAAAAAATcc  >  1:605028/1‑151 (MQ=255)

AAAAGCGCATCCGGTGCTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTTCCGCATTCGGCATTTTGTAAATGGAACGAGGCACTGCGTCTGGTGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCTTCAGCAAGTTGAAAACTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGCAAACCGAATGCCGTGATTTGCCTGGCGACCGGAGCCACGCCATTACTGACGTATCATTATCTGGTAGAAAAAATCC  >  NC_000913/3286194‑3286480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A