Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,379,933	T→C	D22G (GAC→GGC)	zapE ←	divisome ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,379,933	0	T	C	100.0%	176.7 / NA	50	D22G (GAC→GGC)	zapE	divisome ATPase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (26/24); total (26/24)

TGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGTCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATAT  >  NC_000913/3379788‑3380079

tGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGt                                                                                                                                               >  1:491446/1‑151 (MQ=255)

 ggCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGt                                                                                                                                               <  2:723374/150‑1 (MQ=255)

 ggCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGt                                                                                                                                               <  2:589869/150‑1 (MQ=255)

    gTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTTGCCGGGTTGAt                                                                                                                                           <  2:418886/151‑1 (MQ=255)

      aTGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGTGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATgg                                                                                                                                         >  1:500932/1‑151 (MQ=255)

      aTGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATgg                                                                                                                                         <  2:255885/151‑1 (MQ=255)

        gCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCt                                                                                                                                       <  2:568040/151‑1 (MQ=255)

                tCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCAt                                                                                                                               >  2:457508/1‑151 (MQ=255)

                  ttCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCAtt                                                                                                                              >  1:215536/1‑150 (MQ=255)

                   tCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTa                                                                                                                             >  2:157858/1‑150 (MQ=255)

                   tCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTa                                                                                                                             >  2:5731/1‑150 (MQ=255)

                    cgcgTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAg                                                                                                                           <  1:157825/151‑1 (MQ=255)

                       gTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAgcg                                                                                                                         >  2:579150/1‑150 (MQ=255)

                           aCCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCtt                                                                                                                     <  1:506562/150‑1 (MQ=255)

                           aCCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTc                                                                                                                    >  2:376897/1‑151 (MQ=255)

                                  aGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTAtt                                                                                                             <  1:85764/151‑1 (MQ=255)

                                  aGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTAtt                                                                                                             <  1:211271/151‑1 (MQ=255)

                                                  ccATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAAc                                                                                             <  1:702816/151‑1 (MQ=255)

                                                        gCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTg                                                                                       <  1:462317/151‑1 (MQ=255)

                                                            aCTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGCCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATg                                                                                   >  1:268531/1‑151 (MQ=255)

                                                                gTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCt                                                                               <  2:554494/151‑1 (MQ=255)

                                                                         gCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTAt                                                                      <  2:690316/151‑1 (MQ=255)

                                                                            ggtggCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTAtt                                                                     <  1:85381/149‑1 (MQ=255)

                                                                            ggtggCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTcc                                                                   <  2:774639/151‑1 (MQ=255)

                                                                            ggtggCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTc                                                                    <  1:286682/150‑1 (MQ=255)

                                                                             gtggCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGTCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCt                                                                  <  2:487132/151‑1 (MQ=255)

                                                                             gtggCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCt                                                                  <  1:63729/151‑1 (MQ=255)

                                                                               ggCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTg                                                                >  2:253643/1‑151 (MQ=255)

                                                                                 cGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGa                                                               >  2:196635/1‑150 (MQ=255)

                                                                                       aTTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCg                                                        <  2:220200/151‑1 (MQ=255)

                                                                                          gatgaGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTg                                                     >  1:514062/1‑151 (MQ=255)

                                                                                             gaGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTgcgc                                                  >  2:438128/1‑151 (MQ=255)

                                                                                               gTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAAGTATTGCGATGTTGGGGTAACGCATTGCATGATCTTATTGTTATTCCTTGAATAATCGGt                                                      <  1:563224/145‑1 (MQ=255)

                                                                                                            aatTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTg                                   >  2:635868/1‑151 (MQ=255)

                                                                                                                 ccAGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGa                               >  1:93348/1‑150 (MQ=255)

                                                                                                                   aGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGaaa                             >  1:135542/1‑150 (MQ=255)

                                                                                                                   aGGCGGCTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGaaa                             >  1:358475/1‑150 (MQ=255)

                                                                                                                    ggcggcTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGaaaaa                           <  1:769033/151‑1 (MQ=255)

                                                                                                                    ggcggcTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGaaaa                            >  2:151354/1‑150 (MQ=255)

                                                                                                                     gcggcTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGaaaaa                           >  2:444437/1‑150 (MQ=255)

                                                                                                                     gcggcTGACGGCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGaaaaa                           >  2:222136/1‑150 (MQ=255)

                                                                                                                              ggCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTc                 >  1:672914/1‑151 (MQ=255)

                                                                                                                              ggCCTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTc                 >  2:253357/1‑151 (MQ=255)

                                                                                                                                ccTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCt                >  1:293489/1‑150 (MQ=255)

                                                                                                                                ccTCTTTTTGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTa               <  2:363064/151‑1 (MQ=255)

                                                                                                                                 ctctTTTTGAACGTCGCCGGGTTGATGGCTGCATTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTac              >  1:756315/1‑151 (MQ=255)

                                                                                                                                   ctTTTTGATCTTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTac              <  2:477037/149‑1 (MQ=255)

                                                                                                                                     ttttGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTacac            <  2:479825/149‑1 (MQ=255)

                                                                                                                                     ttttGAACGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACt           >  2:584988/1‑150 (MQ=255)

                                                                                                                                          aaCGTCGCCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTAcgcg      >  1:546018/1‑150 (MQ=255)

                                                                                                                                            cgtcgcCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGata   <  2:748373/151‑1 (MQ=255)

                                                                                                                                             gtcgccGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGatat  <  2:619870/151‑1 (MQ=255)

TGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTTTTTGAACGTCGTCGGGTTGATGGCTGCCTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATAT  >  NC_000913/3379788‑3380079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A