Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F50 I2 R1
|
257 |
49.9 |
1554294 |
99.0% |
1538751 |
150.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,434,764 |
T→C |
L184S (TTG→TCG) |
fmt → |
10‑formyltetrahydrofolate:L‑methionyl‑tRNA(fMet) N‑formyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,434,764 | 0 | T | C | 100.0%
| 197.7
/ NA
| 53 | L184S (TTG→TCG) | fmt | 10‑formyltetrahydrofolate:L‑methionyl‑tRNA(fMet) N‑formyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (32/21); total (32/21) |
CTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTCGCGCTTTCAATC > NC_000913/3434617‑3434910
|
cTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGa > 2:33309/1‑150 (MQ=255)
gACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTg > 2:431258/1‑151 (MQ=255)
cATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGc > 1:283389/1‑150 (MQ=255)
aTTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGc > 2:545774/1‑149 (MQ=255)
tATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGac > 2:245542/1‑151 (MQ=255)
aTGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGacg > 2:318935/1‑151 (MQ=255)
cAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGacggc > 1:558656/1‑150 (MQ=255)
ggATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGacggcacggc < 1:756726/150‑1 (MQ=255)
ggATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCg > 2:527826/1‑151 (MQ=255)
gTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGaaa < 2:208356/150‑1 (MQ=255)
gTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAAc < 2:260727/151‑1 (MQ=255)
ggTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAAcc > 2:571464/1‑149 (MQ=255)
gTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAAcc > 1:750192/1‑148 (MQ=255)
aGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGtt > 1:680783/1‑151 (MQ=255)
acacCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCa < 2:558708/151‑1 (MQ=255)
cacCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTc > 1:55568/1‑149 (MQ=255)
cacCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTc > 2:436181/1‑149 (MQ=255)
cacCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTc > 2:2539/1‑149 (MQ=255)
atGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAActc < 1:45468/151‑1 (MQ=255)
gCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCtt > 2:476543/1‑151 (MQ=255)
ataAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCa < 1:660991/150‑1 (MQ=255)
ataAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCAc > 1:309742/1‑151 (MQ=255)
tcCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCg > 1:483546/1‑151 (MQ=255)
ccTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCg < 1:536172/150‑1 (MQ=255)
ccTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCg > 2:227136/1‑150 (MQ=255)
gCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCgaga > 2:276029/1‑150 (MQ=255)
cGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGtt > 1:459986/1‑151 (MQ=255)
gATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTg > 1:141640/1‑151 (MQ=255)
tACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGt > 1:37231/1‑151 (MQ=255)
cTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTa > 2:607903/1‑150 (MQ=255)
agaTACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAaagaagaa < 2:322910/151‑1 (MQ=255)
aCCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAgcg < 1:487378/150‑1 (MQ=255)
ggTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTAtt < 1:704577/150‑1 (MQ=255)
ggTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTg < 2:642199/151‑1 (MQ=255)
cGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTg > 2:773174/1‑151 (MQ=255)
cGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTg < 2:450535/151‑1 (MQ=255)
gCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTgg < 2:415055/151‑1 (MQ=255)
gTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTc > 2:370419/1‑150 (MQ=255)
gTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCa < 1:436130/151‑1 (MQ=255)
gTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCa < 1:2539/151‑1 (MQ=255)
aCAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTc > 1:111435/1‑151 (MQ=255)
aGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGgc < 2:630083/151‑1 (MQ=255)
tGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGgcag > 1:623345/1‑150 (MQ=255)
gCAGAGCTTGGCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGcac < 1:760624/151‑1 (MQ=255)
ggCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGTGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGaa < 1:598791/150‑1 (MQ=255)
gCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACg < 2:722643/151‑1 (MQ=255)
gCCCACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACg > 2:353654/1‑151 (MQ=255)
cccACAAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGc > 1:33884/1‑151 (MQ=255)
cacaAGGGCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTg < 1:596910/151‑1 (MQ=255)
ggCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTcg > 2:598392/1‑151 (MQ=255)
ggCTTATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTcg > 2:428667/1‑151 (MQ=255)
tATCACCACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTCGCGCt > 2:476266/1‑151 (MQ=255)
acGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTCGCGCTTTCAATc < 2:344665/151‑1 (MQ=255)
|
CTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTCGCGCTTTCAATC > NC_000913/3434617‑3434910
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
