Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,434,764	T→C	L184S (TTG→TCG)	fmt →	10‑formyltetrahydrofolate:L‑methionyl‑tRNA(fMet) N‑formyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,434,764	0	T	C	100.0%	106.4 / NA	31	L184S (TTG→TCG)	fmt	10‑formyltetrahydrofolate:L‑methionyl‑tRNA(fMet) N‑formyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/17); total (14/17)

GTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTTGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTCGCG  >  NC_000913/3434622‑3434901

gtgACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAaacaac                                                                                                                                    >  2:207880/1‑151 (MQ=255)

gtgACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAaacaa                                                                                                                                     <  2:825097/150‑1 (MQ=255)

 tgACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACt                                                                                                                                   >  2:388376/1‑151 (MQ=255)

   aCCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCGGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTgg                                                                                                                                 <  2:230667/151‑1 (MQ=255)

   aCCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTgg                                                                                                                                 >  1:699710/1‑151 (MQ=255)

            cAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGacggc                                                                                                                         >  1:403810/1‑150 (MQ=255)

             aaaTGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGacggcac                                                                                                                       >  2:639678/1‑151 (MQ=255)

               aTGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGacggcacgg                                                                                                                     <  1:10092/151‑1 (MQ=255)

                       cGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAAcc                                                                                                              >  2:19342/1‑150 (MQ=255)

                       cGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCa                                                                                                             <  1:809150/151‑1 (MQ=255)

                           ttAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGa                                                                                                           >  2:676012/1‑149 (MQ=255)

                                      tGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGa                                                                                               >  2:148795/1‑150 (MQ=255)

                                       gATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGATGaa                                                                                              <  1:512002/150‑1 (MQ=255)

                                             ctctATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTg                                                                                      >  1:713569/1‑151 (MQ=255)

                                                          ccTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATTACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCga                                                                         <  2:728785/151‑1 (MQ=255)

                                                          ccTGCCCGATT‑CTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGcc                                                                           <  2:86138/148‑1 (MQ=255)

                                                                  aTTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCGAGAAGTTg                                                                  >  1:237184/1‑150 (MQ=255)

                                                                        gCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAATGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCTCTTACGCCGAGAAGTTGAGTaa                                                             <  1:301567/149‑1 (MQ=255)

                                                                              gaTACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCGAGAAGTTGAGTAaagaagaag                                                     <  1:88153/151‑1 (MQ=255)

                                                                               aTACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAgc                                                    <  1:812468/151‑1 (MQ=255)

                                                                               aTACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAgc                                                    <  2:787547/151‑1 (MQ=255)

                                                                                       ggTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTAtt                                             <  1:416960/150‑1 (MQ=255)

                                                                                         tACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGAc                                          >  1:661673/1‑151 (MQ=255)

                                                                                           cGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTg                                        <  2:287946/151‑1 (MQ=255)

                                                                                                 aCGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACt                                  >  1:3004/1‑151 (MQ=255)

                                                                                                    aCAAGCTGACAGAGCCTGTCCCACAAGGGCTTATCTCC‑CCGTCGAAACAACTGGCAGACGGCACGGCGATACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCGAGATGTTGGGTAAAGAATAGGGGCGTATTGACTGGTCTCttt                                <  1:613126/150‑1 (MQ=255)

                                                                                                      aggCTGGAAGAGCTTGGCCCACAA‑GGCTTAACCCCAAGGCCAAAACAATGGGCAGCCGGACCGGCG‑AACCAAAAAGTCAAGACCAAACCCCTTGTC‑CTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCg                              <  2:203696/147‑1 (MQ=255)

                                                                                                      aaGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCgg                             >  1:24859/1‑151 (MQ=255)

                                                                                                                    tGGCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGa                <  2:798691/150‑1 (MQ=255)

                                                                                                                     ggCCCACAAGGGCTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAAc              <  1:639636/151‑1 (MQ=255)

                                                                                                                                 cTTATCACC‑ACGTCGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTcgcg  >  2:548290/1‑151 (MQ=255)

GTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACC‑ACGTTGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAA‑CTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTCGCG  >  NC_000913/3434622‑3434901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A