Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	969,078	T→C	I238T (ATT→ACT)	lpxK →	lipid A 4'kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	969,078	0	T	C	100.0%	109.7 / NA	37	I238T (ATT→ACT)	lpxK	lipid A 4'kinase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/18); total (19/18)

GCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTG  >  NC_000913/968942‑969221

gCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATccgcc                                                                                                                                     >  1:100926/1‑149 (MQ=255)

  ggTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTCCCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCAGCCGCcgcg                                                                                                                                  <  1:763516/150‑1 (MQ=255)

    tAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGGCTGGGCCTCCGCCGCGCtt                                                                                                                               >  2:706806/1‑151 (MQ=255)

             aCGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCAc                                                                                                                      <  2:53118/151‑1 (MQ=255)

               ggcggTGTCCCTCGCCGCGGTGAAAGCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGc                                                                                                                    <  1:839808/151‑1 (MQ=255)

                  ggTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTg                                                                                                                  <  1:100945/150‑1 (MQ=255)

                   gtgtCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGa                                                                                                                 >  2:292808/1‑150 (MQ=255)

                    tgtCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGa                                                                                                                 >  2:624911/1‑149 (MQ=255)

                     gtCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGa                                                                                                                 >  2:568023/1‑148 (MQ=255)

                         cTCGCCGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACCTGTAGTGGCCATCGCGGGGACTGGGCATGCGCCGGGCTTTTTTGCCAAGCTGGAGGtgcg                                                                                                          >  1:455791/1‑149 (MQ=255)

                            gCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTgg                                                                                                       >  2:431604/1‑151 (MQ=255)

                                          cccATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGaa                                                                                          >  1:623083/1‑150 (MQ=255)

                                           ccATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGaaaa                                                                                        <  1:783887/151‑1 (MQ=255)

                                               gCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAAtgt                                                                                    <  1:484514/151‑1 (MQ=255)

                                                cATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAAtgtg                                                                                   >  2:392885/1‑151 (MQ=255)

                                                 aTCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAAtgtg                                                                                   >  1:217496/1‑150 (MQ=255)

                                                         ccGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTgg                                                                          >  1:424776/1‑151 (MQ=255)

                                                                 ggCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGatcat                                                                  <  2:212782/151‑1 (MQ=255)

                                                                 ggCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGatcat                                                                  <  2:73648/151‑1 (MQ=255)

                                                                             aCGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAAc                                                      <  2:193058/151‑1 (MQ=255)

                                                                                 aCCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATg                                                  >  1:610316/1‑151 (MQ=255)

                                                                                      tACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCgg                                               >  1:487088/1‑149 (MQ=255)

                                                                                        cgcgTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGt                                           >  1:331641/1‑151 (MQ=255)

                                                                                              gtgACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTAGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGc                                     <  1:360929/151‑1 (MQ=255)

                                                                                                gACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCg                                    <  2:445487/150‑1 (MQ=255)

                                                                                                       cTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTa                              >  2:439907/1‑149 (MQ=255)

                                                                                                              tGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAGCCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCgg                      <  1:273985/150‑1 (MQ=255)

                                                                                                              tGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCggg                     >  2:313538/1‑151 (MQ=255)

                                                                                                                aaCATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCa                   <  2:456686/151‑1 (MQ=255)

                                                                                                                  cATGTAGTGGTGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCggg                     <  1:257206/147‑1 (MQ=255)

                                                                                                                       aGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTg            <  2:414789/151‑1 (MQ=255)

                                                                                                                        gTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTgg           <  2:534606/151‑1 (MQ=255)

                                                                                                                        gTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTgg           >  1:737039/1‑151 (MQ=255)

                                                                                                                         tGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGCGGTGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGCAAGCGCCGGGCAAAc                >  1:774551/1‑145 (MQ=255)

                                                                                                                             gATGGCGGGTACTGGGCATCCGCCGCGCTTTTTTGCCATGCTGAAGATGTGTGGCGTCCAACCGGAAAAATGTGTACCGCTTGCCGATCATCAGTATTTGAACCATGCGGATGTCAGTTCGTTGGTAATCGCCGGGCAAACTCTgtt          <  1:757499/147‑3 (MQ=255)

                                                                                                                                ggCGGGGACTGGGCATCCGCCGCGCTTTTTTACCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACt   >  1:588300/1‑151 (MQ=255)

                                                                                                                                 gCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTg  <  1:727008/151‑1 (MQ=255)

GCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTG  >  NC_000913/968942‑969221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A