Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	973,216	C→T	L62L (CTG→CTA)	elyC ←	envelope biogenesis factor; DUF218 superfamily protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	973,216	0	C	T	100.0%	132.5 / NA	36	L62L (CTG→CTA)	elyC	envelope biogenesis factor; DUF218 superfamily protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/15); total (21/15)

TCATTCAGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGCAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATCAACAACATCAGCGGAAGTGG  >  NC_000913/973070‑973359

tcattcaGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGc                                                                                                                                             >  1:156717/1‑151 (MQ=255)

  attcaGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCgg                                                                                                                                           <  1:662371/151‑1 (MQ=255)

    tcaGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGt                                                                                                                                          <  1:248667/150‑1 (MQ=255)

     caGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCg                                                                                                                                        <  2:546534/151‑1 (MQ=255)

          atGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGGTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTa                                                                                                                                    >  1:600954/2‑150 (MQ=255)

             gCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCgg                                                                                                                                <  2:338615/151‑1 (MQ=255)

                 aCTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGc                                                                                                                            >  1:491035/1‑151 (MQ=255)

                    gTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCaa                                                                                                                          >  1:268026/1‑150 (MQ=255)

                             tAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATaa                                                                                                                 <  1:634116/150‑1 (MQ=255)

                                       cTCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCgcc                                                                                                       >  1:473932/1‑150 (MQ=255)

                                       cTCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCgcc                                                                                                       >  2:500348/1‑150 (MQ=255)

                                         cGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGTTTGCAAACTCAATAACAAGAGCgcca                                                                                                      <  1:487545/149‑1 (MQ=255)

                                          ggTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCgccagcc                                                                                                   <  2:763351/151‑1 (MQ=255)

                                                 aCTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAAt                                                                                            >  2:827691/1‑151 (MQ=255)

                                                       ggTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCtaat                                                                                      <  2:748445/151‑1 (MQ=255)

                                                        gTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCtaata                                                                                     <  2:794106/151‑1 (MQ=255)

                                                                 ataACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCtt                                                                             <  1:143127/150‑1 (MQ=255)

                                                                 ataACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTc                                                                            >  1:724338/1‑151 (MQ=255)

                                                                   aaCCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTccc                                                                          >  2:138941/1‑151 (MQ=255)

                                                                       accTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGtt                                                                      >  2:338052/1‑151 (MQ=255)

                                                                       accTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGtt                                                                      >  2:30347/1‑151 (MQ=255)

                                                                           ccGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGttttt                                                                   <  2:206855/150‑1 (MQ=255)

                                                                                caccacGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGaaa                                                              <  1:748885/150‑1 (MQ=255)

                                                                                  ccacGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACg                                                           <  2:42035/151‑1 (MQ=255)

                                                                                                aCTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCAcagc                                              >  2:823975/1‑150 (MQ=255)

                                                                                                     ttGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCgcc                                        >  1:747970/1‑151 (MQ=255)

                                                                                                         gAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCgccaggc                                    >  2:235842/1‑151 (MQ=255)

                                                                                                         gAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCgccaggc                                    >  1:444708/1‑151 (MQ=255)

                                                                                                           attattCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCgccaggcc                                   >  2:267448/1‑150 (MQ=255)

                                                                                                              attCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAgcg                               >  2:768060/1‑151 (MQ=255)

                                                                                                                 ccATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCa                            <  1:335697/151‑1 (MQ=255)

                                                                                                                           tAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATcaac                   >  2:39636/1‑150 (MQ=255)

                                                                                                                            aGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATcaacaa                 <  2:851728/151‑1 (MQ=255)

                                                                                                                            aGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATcaaca                  >  2:796276/1‑150 (MQ=255)

                                                                                                                               tGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATCAACAACAt              >  1:570262/1‑151 (MQ=255)

                                                                                                                                           gACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATCAACAACATCAGCGGAAGTgg  >  1:582367/1‑151 (MQ=255)

TCATTCAGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGCAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATCAACAACATCAGCGGAAGTGG  >  NC_000913/973070‑973359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A