Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I3 R1	283	58.3	1819865	98.8%	1798026	150.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	973,216	C→T	L62L (CTG→CTA)	elyC ←	envelope biogenesis factor; DUF218 superfamily protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	973,216	0	C	T	100.0%	197.5 / NA	52	L62L (CTG→CTA)	elyC	envelope biogenesis factor; DUF218 superfamily protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (36/16); total (36/16)

TCATTCAGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGCAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATCAACAACATCAGCGGAAGTGGCAGC  >  NC_000913/973070‑973363

tcattcaGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGc                                                                                                                                                 >  1:38868/1‑151 (MQ=255)

        gCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCTCTGCGGGTTCCAGGTATAACCCCCTCCGAGCCCCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCt                                                                                                                                         <  2:889567/151‑1 (MQ=255)

         cGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCt                                                                                                                                         >  2:669763/1‑150 (MQ=255)

              cAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCggc                                                                                                                                   >  2:253666/1‑150 (MQ=255)

              cAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGt                                                                                                                                   <  2:273863/151‑1 (MQ=255)

                 aCTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGc                                                                                                                                >  1:280415/1‑151 (MQ=255)

                   tGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCa                                                                                                                               >  2:683958/1‑150 (MQ=255)

                                  tAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAag                                                                                                                 >  2:353803/1‑149 (MQ=255)

                                        tCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCgcc                                                                                                           >  1:564630/1‑149 (MQ=255)

                                          ggTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCgccagcc                                                                                                       <  1:765331/151‑1 (MQ=255)

                                               ccACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCAccc                                                                                                   >  1:602388/1‑150 (MQ=255)

                                                          tCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCtaataa                                                                                        <  2:754569/150‑1 (MQ=255)

                                                           ccAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATaaa                                                                                       <  1:94291/150‑1 (MQ=255)

                                                              ggTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATc                                                                                   >  1:624369/1‑151 (MQ=255)

                                                               gTATAACCACCTCCGAGCACCACGATATAGTCCTCTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCt                                                                                  <  1:359977/151‑1 (MQ=255)

                                                                tataACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCtt                                                                                 >  2:836814/1‑151 (MQ=255)

                                                                tataACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCtt                                                                                 >  1:230654/1‑151 (MQ=255)

                                                                       accTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGtt                                                                          >  1:709609/1‑151 (MQ=255)

                                                                        ccTCCGAGCACCACGATATAGTCCGCTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGtt                                                                          <  2:492087/150‑1 (MQ=255)

                                                                        ccTCCGAGCACCACGATATAGTACACTTTTTGCGAATTATTCCATGTCGGATAGGCGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGttt                                                                         >  2:422292/1‑151 (MQ=255)

                                                                           ccGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGtttt                                                                        <  2:549084/149‑1 (MQ=255)

                                                                           ccGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGtttt                                                                        <  1:507028/149‑1 (MQ=255)

                                                                                  ccacGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACg                                                               >  1:22717/1‑151 (MQ=255)

                                                                                     cGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCt                                                            <  1:845139/151‑1 (MQ=255)

                                                                                         ataGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAAc                                                        >  1:242063/1‑151 (MQ=255)

                                                                                          taGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAAcc                                                       >  2:9086/1‑151 (MQ=255)

                                                                                              ccACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACcaca                                                    <  2:47193/150‑1 (MQ=255)

                                                                                                aCTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCAcagc                                                  >  1:352223/1‑150 (MQ=255)

                                                                                                 cTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCAcagc                                                  >  1:48072/1‑149 (MQ=255)

                                                                                                  tttttGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACagcagc                                               >  1:285812/1‑151 (MQ=255)

                                                                                                   ttttGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAgcg                                              >  1:704534/1‑151 (MQ=255)

                                                                                                    tttGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAgcgc                                             >  2:422223/1‑151 (MQ=255)

                                                                                                     ttGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAgcgc                                             <  2:328584/150‑1 (MQ=255)

                                                                                                     ttGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCgcc                                            >  1:421989/1‑151 (MQ=255)

                                                                                                        cGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCgccagg                                         >  2:76870/1‑151 (MQ=255)

                                                                                                               ttCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAgcgc                                  >  1:507537/1‑151 (MQ=255)

                                                                                                                tCCATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGcc                                 >  2:872104/1‑151 (MQ=255)

                                                                                                                 ccATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGcc                                 >  2:699462/1‑150 (MQ=255)

                                                                                                                  cATGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCa                                >  2:462762/1‑150 (MQ=255)

                                                                                                                   aTGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCaat                              >  2:489740/1‑151 (MQ=255)

                                                                                                                    tGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCaat                              >  1:158240/1‑150 (MQ=255)

                                                                                                                    tGTCGGATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCaat                              >  1:391111/1‑150 (MQ=255)

                                                                                                                        ggATAGGTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCaata                             >  1:315647/1‑147 (MQ=255)

                                                                                                                             ggTGCTTTCGATGGGACGCAGTAGGTGGTCGGCTACCGGTTACAAACTCAATAACAAGAGCGACAGCCACCCAATGCTAATAAAAACCTTCCCGGTTTTTTGAAAACGGCTAAACCTCAGCAGAGCCAGGCCAGCGCCAATAATcaacaa                     <  2:869615/150‑1 (MQ=255)

                                                                                                                             ggTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATCaacaac                    <  2:322367/151‑1 (MQ=255)

                                                                                                                              gTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATCaacaac                    >  1:717884/1‑150 (MQ=255)

                                                                                                                              gTGCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATCaacaac                    >  1:22028/1‑150 (MQ=255)

                                                                                                                                gCTTTCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCTGGCCAGCGCCAATAATCAACAACATc                 <  2:517323/151‑1 (MQ=255)

                                                                                                                                  tttCGATGGGACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATCAACAACATc                 >  1:430163/1‑149 (MQ=255)

                                                                                                                                          ggACGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATCAACAACATCAGCGGAAGTg       <  1:86733/151‑1 (MQ=255)

                                                                                                                                             cGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATCAACAACATCAGCGGAAGTGgc     >  2:630709/1‑150 (MQ=255)

                                                                                                                                             cGCAGTAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATCAACAACATCAGCGGAAGTGgc     >  2:274057/1‑150 (MQ=255)

                                                                                                                                              gcagtaGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATCAACAACATCAGCGGAAGTGgcag   <  1:738612/151‑1 (MQ=255)

                                                                                                                                               cagtagGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATCAACAACATCAGCGGAAGTGgcagc  <  1:353829/151‑1 (MQ=255)

                                                                                                                                               cagtagGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATCAACAACATCAGCGGAAGTGgcag   >  1:637327/1‑150 (MQ=255)

TCATTCAGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAACCACCTCCGAGCACCACGATATAGTCCACTTTTTGCGAATTATTCCATGTCGGATAGGTGCTTTCGATGGGACGCAGCAGGCGGTCGGCTACCGGTTGCAAACTCAATAACAAGAGCGCCAGCCACCCAATGCTAATAAAAATCTTCCCGGTTTTTTGAAAACGGCTAAACCACAGCAGCGCCAGGCCAGCGCCAATAATCAACAACATCAGCGGAAGTGGCAGC  >  NC_000913/973070‑973363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A