Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,010,983	C→T	A340A (GCC→GCT)	uup →	replication regulatory ABC‑F family DNA‑binding ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,010,983	0	C	T	97.8%	159.0 / ‑3.7	46	A340A (GCC→GCT)	uup	replication regulatory ABC‑F family DNA‑binding ATPase
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (20/24); total (20/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.21e-01

CGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCG  >  NC_000913/1010837‑1011125

cGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAgg                                                                                                                                            >  1:521208/1‑151 (MQ=255)

 gTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCGGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGt                                                                                                                                           <  2:68998/151‑1 (MQ=255)

 gTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGt                                                                                                                                           >  2:607774/1‑151 (MQ=255)

            ggtgAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCg                                                                                                                                <  1:191797/151‑1 (MQ=255)

            ggtgAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCACAAGTTCTACGTGGCg                                                                                                                                <  1:856790/151‑1 (MQ=255)

                     cgcgAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAAtt                                                                                                                        <  2:21486/150‑1 (MQ=255)

                             gATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGAtt                                                                                                               <  1:732633/151‑1 (MQ=255)

                               tGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTgg                                                                                                             >  2:117707/1‑151 (MQ=255)

                                gggTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGt                                                                                                            >  1:319458/1‑151 (MQ=255)

                                    aCCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCg                                                                                                         <  1:739991/150‑1 (MQ=255)

                                      cGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAAt                                                                                                      <  2:17079/151‑1 (MQ=255)

                                           aaaTGCAGGTGGAAGAGGCTAGCCGAGCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCATGTTAACGGTAAGCAACTTGTGAAAGATTTGTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTg                                                                                                 <  1:229838/149‑1 (MQ=255)

                                           aGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTg                                                                                                 <  1:100716/151‑1 (MQ=255)

                                                cAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGc                                                                                             >  1:828124/1‑150 (MQ=255)

                                                 aGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCa                                                                                            >  2:444639/1‑150 (MQ=255)

                                                 aGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCa                                                                                            >  2:483146/1‑150 (MQ=255)

                                                     ggAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAAcc                                                                                       >  1:793857/1‑151 (MQ=255)

                                                     ggAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAAc                                                                                        <  1:68986/150‑1 (MQ=255)

                                                            gCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACgctgc                                                                                >  1:338117/1‑151 (MQ=255)

                                                            gCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACgctgc                                                                                <  2:527139/151‑1 (MQ=255)

                                                             ccAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACgctgct                                                                               <  1:484917/151‑1 (MQ=255)

                                                                   gCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACt                                                                         <  1:115581/151‑1 (MQ=255)

                                                                            aaaTCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCgg                                                                >  2:14444/1‑151 (MQ=255)

                                                                             aaTCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGt                                                               >  1:727927/1‑151 (MQ=255)

                                                                                cGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAg                                                            <  2:403769/151‑1 (MQ=255)

                                                                                   tttCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCtt                                                         >  2:53476/1‑151 (MQ=255)

                                                                                   tttCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCt                                                          <  1:442911/150‑1 (MQ=255)

                                                                                     tCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTc                                                        <  1:119906/150‑1 (MQ=255)

                                                                                      cGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCa                                                       >  2:194477/1‑150 (MQ=255)

                                                                                         aaTGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCg                                                   <  1:525452/151‑1 (MQ=255)

                                                                                             gAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGAc                                                >  1:334577/1‑150 (MQ=255)

                                                                                                    tttGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCg                                        >  2:717656/1‑151 (MQ=255)

                                                                                                     ttGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCg                                        >  1:267296/1‑150 (MQ=255)

                                                                                                       gCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTAt                                     <  2:695513/151‑1 (MQ=255)

                                                                                                        cTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTAtt                                    >  2:250356/1‑151 (MQ=255)

                                                                                                           ccAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCAc                                 <  2:724357/151‑1 (MQ=255)

                                                                                                                 tAACGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGc                           >  1:632220/1‑151 (MQ=255)

                                                                                                                  aaCGGTAAGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCa                          <  2:599958/151‑1 (MQ=255)

                                                                                                                   aCGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCAc                         <  2:385415/151‑1 (MQ=255)

                                                                                                                    cGGTAAGCAACTGGGGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAACTGCCCTTATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCAcc                        >  1:119002/1‑151 (MQ=255)

                                                                                                                        aaGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCaaa                     <  1:835916/150‑1 (MQ=255)

                                                                                                                        aaGCAACTGGTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAAc                    <  2:632025/151‑1 (MQ=255)

                                                                                                                                ggTGAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTg            >  2:398490/1‑151 (MQ=255)

                                                                                                                                   gAAAGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGc          <  1:761200/150‑1 (MQ=255)

                                                                                                                                    aaaGATTTTTCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCtt        <  2:763537/151‑1 (MQ=255)

                                                                                                                                           tttCTGCTCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCg  <  2:625673/150‑1 (MQ=255)

CGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCG  >  NC_000913/1010837‑1011125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A