Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,022,227	A→G	Y553H (TAT→CAT)	yccS ←	putative transporter, FUSC superfamily inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,022,227	0	A	G	100.0%	132.7 / NA	37	Y553H (TAT→CAT)	yccS	putative transporter, FUSC superfamily inner membrane protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/17); total (20/17)

GGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCAAAACCTTC  >  NC_000913/1022087‑1022374

ggTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTg                                                                                                                                           <  1:426951/151‑1 (MQ=255)

ggTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTg                                                                                                                                           <  1:73227/151‑1 (MQ=255)

        tCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCtg                                                                                                                                    <  1:77576/150‑1 (MQ=255)

          ggCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTgg                                                                                                                                 >  2:692243/1‑151 (MQ=255)

               gCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGt                                                                                                                            >  1:620034/1‑151 (MQ=255)

                  ggACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTc                                                                                                                          <  1:376173/150‑1 (MQ=255)

                   gACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCg                                                                                                                         >  2:193482/1‑150 (MQ=255)

                      atatTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCa                                                                                                                     <  1:673008/151‑1 (MQ=255)

                      atatTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAAATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGc                                                                                                                      >  2:311444/1‑150 (MQ=255)

                       tatTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGCTACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCAt                                                                                                                    >  1:746876/1‑151 (MQ=255)

                        atTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATg                                                                                                                   >  1:161695/1‑151 (MQ=255)

                              tACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCgg                                                                                                              >  1:478486/1‑150 (MQ=255)

                                   ccGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGAtt                                                                                                        >  2:412098/1‑151 (MQ=255)

                                     gACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGc                                                                                                      >  1:621339/1‑151 (MQ=255)

                                           aGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACt                                                                                                <  2:513059/151‑1 (MQ=255)

                                               cAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCa                                                                                            <  1:115555/151‑1 (MQ=255)

                                                 gCATCACGGTTGTGTGCATCGCGGCGGGCAATAGGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGt                                                                                          <  2:160648/151‑1 (MQ=255)

                                                 gCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGt                                                                                          <  2:319150/151‑1 (MQ=255)

                                                             tgtgCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGGATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCACTCAGGCCAGATGt                                                                              >  1:384838/1‑151 (MQ=255)

                                                                              aaTACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCAc                                                             <  2:815778/151‑1 (MQ=255)

                                                                               atacgatacgCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACg                                                            <  2:784920/151‑1 (MQ=255)

                                                                                 acgatacgCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCa                                                          <  2:416453/151‑1 (MQ=255)

                                                                                       cgCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTTGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCACACGCAATgt                                                      <  1:483002/149‑2 (MQ=255)

                                                                                          cataCGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGcaca                                                  >  2:595935/4‑150 (MQ=255)

                                                                                          cAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACaa                                                 <  2:400238/151‑1 (MQ=255)

                                                                                           aGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAAc                                                >  2:282835/1‑151 (MQ=255)

                                                                                              cGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCa                                              >  2:679201/1‑150 (MQ=255)

                                                                                                             tGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGAtt                               >  2:461416/1‑150 (MQ=255)

                                                                                                                    aTTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAg                        <  1:555614/150‑1 (MQ=255)

                                                                                                                       gCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCTGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTa                     >  2:690335/1‑150 (MQ=255)

                                                                                                                       gCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTa                     >  2:575851/1‑150 (MQ=255)

                                                                                                                        cTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAAc                   >  2:158012/1‑151 (MQ=255)

                                                                                                                            aGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCt                >  2:337676/1‑150 (MQ=255)

                                                                                                                                  gCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCaa         <  2:298201/151‑1 (MQ=255)

                                                                                                                                  gCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCa          >  1:715398/1‑150 (MQ=255)

                                                                                                                                   cATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCaa         >  2:172164/1‑150 (MQ=255)

                                                                                                                                         gaTGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCAAAACCTTc  <  1:692200/151‑1 (MQ=255)

GGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCAAAACCTTC  >  NC_000913/1022087‑1022374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A