Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,028,234	A→G	T97A (ACT→GCT)	yccU →	putative CoA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,028,234	0	A	G	98.0%	176.0 / NA	51	T97A (ACT→GCT)	yccU	putative CoA‑binding protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (28/22); minor base C (1/0); total (29/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAACTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGCCAAATAAAAAATCCCCGG  >  NC_000913/1028088‑1028370

cAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGGTGGCAGACGTTCCGGACAAAGTCGATATGGACGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGACCTTGGCGAGAAACctc                                                                                                                                       >  1:160153/1‑150 (MQ=255)

     gTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTgg                                                                                                                                  >  2:601683/1‑150 (MQ=255)

     gTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGa                                                                                                                                 <  2:4903/151‑1 (MQ=255)

           ggCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAAc                                                                                                                           <  2:372041/151‑1 (MQ=255)

                      gctgGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGAt                                                                                                                 <  1:16845/150‑1 (MQ=255)

                        tgGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTa                                                                                                               >  1:263525/1‑150 (MQ=255)

                         gggCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAAt                                                                                                             >  2:368716/1‑151 (MQ=255)

                           gCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGCGATTAATg                                                                                                            >  1:40906/1‑150 (MQ=255)

                               gAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCaa                                                                                                       <  2:362608/151‑1 (MQ=255)

                                     gTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGc                                                                                                  >  2:545009/1‑150 (MQ=255)

                                      tATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGc                                                                                                  >  2:453792/1‑149 (MQ=255)

                                      tATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGc                                                                                                  >  1:513884/1‑149 (MQ=255)

                                         ggCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTAc                                                                                             <  2:519784/151‑1 (MQ=255)

                                            aCGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTgg                                                                                          <  2:286868/151‑1 (MQ=255)

                                              gCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGc                                                                                         >  2:130172/1‑150 (MQ=255)

                                               cTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCAc                                                                                       >  2:337079/1‑151 (MQ=255)

                                                tGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACg                                                                                      >  2:231385/1‑151 (MQ=255)

                                                 ggCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACgg                                                                                     >  1:250657/1‑151 (MQ=255)

                                                    aGACGTTCCGGAAAAAGCCGATATGGTCGATGTTTTCCGCAATTCAGAACCGGCGTGGGGCGTGGCACAGTAAGCTATTGCCATTGGCGCGAAAGCCCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACggg                                                                                    >  1:303899/1‑149 (MQ=255)

                                                     gACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATg                                                                                 >  2:14934/1‑151 (MQ=255)

                                                      aCGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGc                                                                                <  2:568576/151‑1 (MQ=255)

                                                         ttCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCgg                                                                             >  2:70461/1‑151 (MQ=255)

                                                           ccGGAAAAAGTCAATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCGCAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTTCTGGCACGGGATGCCgt                                                                             <  1:770582/149‑2 (MQ=255)

                                                               aaaaaGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTCCTGGCACGGGATGCCGGGTTaaa                                                                       <  1:530760/151‑1 (MQ=255)

                                                                 aaaGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAAtg                                                                     >  2:713269/1‑151 (MQ=255)

                                                                 aaaGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAAtg                                                                     <  1:544668/151‑1 (MQ=255)

                                                                       gATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATgtggtg                                                                >  2:83013/1‑150 (MQ=255)

                                                                       gATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGa                                                               <  1:452045/151‑1 (MQ=255)

                                                                          atGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATgg                                                            <  2:752024/151‑1 (MQ=255)

                                                                             gTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATc                                                         <  1:816722/151‑1 (MQ=255)

                                                                               cGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGc                                                       >  1:482813/1‑151 (MQ=255)

                                                                                gATGTTTTCCGTAATTCAGAAGCGGCGTGGGGTGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCt                                                      >  1:30495/1‑151 (MQ=255)

                                                                                 aTGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTg                                                     >  2:688697/1‑151 (MQ=255)

                                                                                    ttttCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGa                                                                                   <  1:113915/118‑1 (MQ=255)

                                                                                    ttttCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGa                                                                                   >  2:113938/1‑118 (MQ=255)

                                                                                       tCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCgg                                                <  1:30614/150‑1 (MQ=255)

                                                                                        ccGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGc                                               <  2:616201/150‑1 (MQ=255)

                                                                                           tAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTAtt                                           >  2:46099/1‑151 (MQ=255)

                                                                                             aTTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTga                                         <  2:490729/151‑1 (MQ=255)

                                                                                                 aGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGGGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTg                                          >  1:730551/1‑146 (MQ=255)

                                                                                                            ggggcgtggCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCgcctgg                           <  1:46077/150‑1 (MQ=255)

                                                                                                                 gtggCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCgcctgggcctgg                     >  1:15015/1‑151 (MQ=255)

                                                                                                                  tggCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGc                    >  1:361118/1‑151 (MQ=255)

                                                                                                                   ggCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGcc                   <  2:327688/151‑1 (MQ=255)

                                                                                                                   ggCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGc                    <  1:544972/150‑1 (MQ=255)

                                                                                                                       caGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGCCAAAt               >  2:49878/1‑151 (MQ=255)

                                                                                                                             gCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGCCAAATaaaaa          >  2:26429/1‑150 (MQ=255)

                                                                                                                               tATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGCCAAATAAAAAATc       >  2:129713/1‑151 (MQ=255)

                                                                                                                                   gCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGCCAAATAAAAAATCCCCg   <  1:31822/151‑1 (MQ=255)

                                                                                                                                     cATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGCCAAATAAAAAATCCCCgg  >  1:555261/1‑150 (MQ=255)

                                                                                                                                      aTTGGCGCGAAAGCTGTCTGGATGCAACTGGGGTTGATTAATGAGCAAGCGGCAGTACGGGCGCGGGATGCTGGGTTAAATGTGCTTATGGATCGCTGGCCGTCTATTGAGATCGCTCGCCTGGGGCTGGCCAAATAAAAAATCCCCgg  <  1:568796/149‑1 (MQ=255)

CAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAACTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGCCAAATAAAAAATCCCCGG  >  NC_000913/1028088‑1028370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A