Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,237,152	T→C	A30A (GCA→GCG)	ycgB ←	SpoVR family stationary phase protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,237,152	0	T	C	100.0%	169.4 / NA	49	A30A (GCA→GCG)	ycgB	SpoVR family stationary phase protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (28/21); total (28/21)

ACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAGCACCTCATTTCAAGCAT  >  NC_000913/1237017‑1237296

aCCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATcc                                                                                                                                   >  2:68492/1‑151 (MQ=255)

 ccGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATcc                                                                                                                                   <  2:639428/150‑1 (MQ=255)

       gACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGt                                                                                                                            <  1:398540/151‑1 (MQ=255)

         ccAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCg                                                                                                                          >  2:9660/1‑151 (MQ=255)

         ccAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCg                                                                                                                          <  2:379852/151‑1 (MQ=255)

          cAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGa                                                                                                                         <  2:576121/151‑1 (MQ=255)

           aGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGaa                                                                                                                        <  2:791131/151‑1 (MQ=255)

                   tAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGt                                                                                                                >  1:60228/1‑151 (MQ=255)

                     gTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCg                                                                                                              <  1:68912/151‑1 (MQ=255)

                         aTTGGCATGCCGACGCTGGAGTAGGCATACATCATTTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGAATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCCGATAAACATCAAGCAGGTCGAACGTCCAGTCGGGTc                                                                                                          >  1:559501/1‑151 (MQ=255)

                              cATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCg                                                                                                     >  2:57/1‑151 (MQ=255)

                                   cGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCa                                                                                                 >  2:501337/1‑150 (MQ=255)

                                    gACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCaa                                                                                                >  1:67560/1‑150 (MQ=255)

                                      cGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCaaa                                                                                               <  1:619387/149‑1 (MQ=255)

                                          ggAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGtgtg                                                                                         >  2:111780/1‑151 (MQ=255)

                                               aGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTc                                                                                    <  2:535494/151‑1 (MQ=255)

                                                    tccatcATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTAt                                                                               >  1:661759/1‑151 (MQ=255)

                                                     ccatcATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAACCATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTAtt                                                                              >  2:31381/1‑151 (MQ=255)

                                                       atcatcTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTc                                                                             >  2:182129/1‑150 (MQ=255)

                                                       atcatcTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTc                                                                             >  1:352053/1‑150 (MQ=255)

                                                              gTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAat                                                                      <  1:231184/150‑1 (MQ=255)

                                                                         aTCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGcc                                                           <  2:72794/150‑1 (MQ=255)

                                                                            aCTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATAc                                                       <  2:490696/151‑1 (MQ=255)

                                                                               tCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATAcgc                                                     <  1:120945/150‑1 (MQ=255)

                                                                                 aaTCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACgcgc                                                   >  1:574368/1‑150 (MQ=255)

                                                                                 aaTCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCa                                                  <  1:182106/151‑1 (MQ=255)

                                                                                    cTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCt                                               >  2:291022/1‑151 (MQ=255)

                                                                                         gCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCAtt                                           <  2:27646/150‑1 (MQ=255)

                                                                                          cGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCAttgt                                         >  2:527583/1‑151 (MQ=255)

                                                                                           gggTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCAttgtt                                        >  2:583302/1‑151 (MQ=255)

                                                                                            ggtaggtaTCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCAttgttg                                       <  2:385853/151‑1 (MQ=255)

                                                                                              taggtaTCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTc                                     >  2:690155/1‑151 (MQ=255)

                                                                                               aggtaTCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCg                                    >  2:698496/1‑151 (MQ=255)

                                                                                                 gtaTCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCgg                                   >  1:678422/1‑150 (MQ=255)

                                                                                                  taTCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGgcg                                 >  1:174443/1‑151 (MQ=255)

                                                                                                            gTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCtgtg                        >  1:409304/1‑150 (MQ=255)

                                                                                                                agTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAg                   <  1:249576/151‑1 (MQ=255)

                                                                                                                  tttCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAGc                  <  2:220136/150‑1 (MQ=255)

                                                                                                                   ttCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAGCa                 <  1:664845/150‑1 (MQ=255)

                                                                                                                   ttCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAGCAc                >  2:214283/1‑151 (MQ=255)

                                                                                                                     cGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAGCACCt              >  1:68290/1‑151 (MQ=255)

                                                                                                                       ccACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAGCACCTc             <  1:500435/150‑1 (MQ=255)

                                                                                                                       ccACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAGCACCTCa            <  1:42490/151‑1 (MQ=255)

                                                                                                                        cACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAGCACCTCa            >  2:343406/1‑150 (MQ=255)

                                                                                                                        cACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAGCACCTCAt           >  1:695544/1‑151 (MQ=255)

                                                                                                                          cccGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAGCACCTCAttt         <  2:708025/151‑1 (MQ=255)

                                                                                                                             ggTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAGCACCTCATTTCa       >  1:71705/1‑150 (MQ=255)

                                                                                                                                 tATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAGCACCTCATTTCAAGCAt  >  2:141160/1‑151 (MQ=255)

                                                                                                                                  aTCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAGCACCTCATTTCAAGCAt  >  2:265841/1‑150 (MQ=255)

                                                                                                                                    ctccGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAGCACCTCATTTCAAGCAt  >  1:139598/1‑148 (MQ=255)

ACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAGCACCTCATTTCAAGCAT  >  NC_000913/1237017‑1237296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A