Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I1 R1 300 54.7 1718999 98.4% 1691495 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,333,046 A→G T667A (ACG→GCG)  topA → DNA topoisomerase I, omega subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,333,0460AG100.0% 204.7 / NA 58T667A (ACG→GCG) topADNA topoisomerase I, omega subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/33);  total (25/33)

TCTGGCTATGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCT  >  NC_000913/1332905‑1333193
                                                                                                                                             |                                                                                                                                                   
tCTGGCTATGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGcggcgcggcg                                                                                                                                                <  1:13462/147‑1 (MQ=255)
tCTGGCTATGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATg                                                                                                                                             <  2:401147/150‑1 (MQ=255)
 cTGGCTATGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATg                                                                                                                                             <  1:168145/149‑1 (MQ=255)
   ggCTATGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGAc                                                                                                                                          >  2:284866/1‑150 (MQ=255)
    gCTATGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGAc                                                                                                                                          >  1:595486/1‑149 (MQ=255)
     cTATGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGc                                                                                                                                       >  1:730503/1‑151 (MQ=255)
        tGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTAt                                                                                                                                    >  1:468726/1‑151 (MQ=255)
          cgcTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTAtct                                                                                                                                  <  2:433/151‑1 (MQ=255)
             tGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCAt                                                                                                                               >  1:206420/1‑151 (MQ=255)
              gccgccGAAAGAGCGTTGCAAAGCCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCGCGAAATGCGGCGCGGCGATGGACAGCTATCTCAt                                                                                                                               <  2:253523/150‑1 (MQ=255)
              gccgccGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCAt                                                                                                                               <  2:194204/150‑1 (MQ=255)
              gccgccGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATc                                                                                                                              <  1:628023/151‑1 (MQ=255)
                 gccgAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGAt                                                                                                                           >  2:740342/1‑151 (MQ=255)
                    gAAAGAGCGTTGCAAAACCATCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCg                                                                                                                        <  2:134045/151‑1 (MQ=255)
                      aaGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGaa                                                                                                                      <  2:695630/151‑1 (MQ=255)
                           cGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGt                                                                                                                  >  1:803770/1‑150 (MQ=255)
                               gCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGt                                                                                                              <  1:721462/150‑1 (MQ=255)
                                  aaaCCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGc                                                                                                           >  2:24464/1‑150 (MQ=255)
                                  aaaCCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCa                                                                                                          <  1:313751/151‑1 (MQ=255)
                                   aaCCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCAt                                                                                                         >  2:783939/1‑151 (MQ=255)
                                    accaccATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATg                                                                                                        >  2:609821/1‑151 (MQ=255)
                                      caccaTTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTc                                                                                                      <  1:661820/151‑1 (MQ=255)
                                       accaTTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCt                                                                                                     <  2:554156/151‑1 (MQ=255)
                                        ccaTTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCtg                                                                                                    <  1:73405/151‑1 (MQ=255)
                                        ccaTTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCtg                                                                                                    <  2:344662/151‑1 (MQ=255)
                                            tAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTgg                                                                                                 <  1:247908/150‑1 (MQ=255)
                                               ccTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGtaat                                                                                             <  2:208445/151‑1 (MQ=255)
                                                    tGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAAccc                                                                                        >  1:180186/1‑151 (MQ=255)
                                                       cGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAAc                                                                                     >  2:665764/1‑151 (MQ=255)
                                                         gAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCt                                                                                   <  1:522285/151‑1 (MQ=255)
                                                         gAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCt                                                                                   <  2:18674/151‑1 (MQ=255)
                                                          aaaaCGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTg                                                                                  <  2:706263/151‑1 (MQ=255)
                                                          aaaaCGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTg                                                                                  >  1:407745/1‑151 (MQ=255)
                                                           aaaCGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGc                                                                                 <  2:733729/151‑1 (MQ=255)
                                                            aaCGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCg                                                                                <  2:683317/151‑1 (MQ=255)
                                                              cGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGAc                                                                              >  1:661668/1‑151 (MQ=255)
                                                               gAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACg                                                                             >  2:639096/1‑151 (MQ=255)
                                                                 aGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGt                                                                           <  1:575242/151‑1 (MQ=255)
                                                                   tGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGtt                                                                          >  2:562190/1‑150 (MQ=255)
                                                                     cTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTAc                                                                        <  2:104306/150‑1 (MQ=255)
                                                                     cTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACg                                                                       >  1:799846/1‑151 (MQ=255)
                                                                     cTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACg                                                                       >  1:280500/1‑151 (MQ=255)
                                                                       gAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACga                                                                      <  1:562152/150‑1 (MQ=255)
                                                                                ggAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAaga                                                             <  1:760964/150‑1 (MQ=255)
                                                                                 gAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAgg                                                           <  2:803826/151‑1 (MQ=255)
                                                                                 gAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAgg                                                           >  2:330185/1‑151 (MQ=255)
                                                                                      cGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGaa                                                      <  2:177405/151‑1 (MQ=255)
                                                                                        aaGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAAtt                                                    >  2:195312/1‑151 (MQ=255)
                                                                                         aGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTc                                                   >  2:620141/1‑151 (MQ=255)
                                                                                                gAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCAtt                                             >  2:748771/1‑150 (MQ=255)
                                                                                                gAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCAtt                                             <  1:718787/150‑1 (MQ=255)
                                                                                                  acccAACGCGCTGCGCGCAAAACGTGGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAAACCAACCTGCGACAGTTACGAGATCGAAGAGGGCGAATTCCGCATTaaa                                          <  1:857170/149‑1 (MQ=255)
                                                                                                  aaCCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTaaa                                          <  1:726847/151‑1 (MQ=255)
                                                                                                  aaCCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTaa                                           >  1:185596/1‑150 (MQ=255)
                                                                                                   aCCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTaa                                           >  2:427086/1‑149 (MQ=255)
                                                                                                        cgcgCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTAt                                    <  1:734681/151‑1 (MQ=255)
                                                                                                                    aaaaCGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATc                        >  2:387144/1‑151 (MQ=255)
                                                                                                                                     aaTGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAAtg       <  2:40111/151‑1 (MQ=255)
                                                                                                                                        gcggcgcggcGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAAtgtg     <  2:646337/150‑1 (MQ=255)
                                                                                                                                        gcggcgcggcGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAAtgtg     <  2:186016/150‑1 (MQ=255)
                                                                                                                                          ggCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGct  <  2:44903/151‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
TCTGGCTATGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCT  >  NC_000913/1332905‑1333193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A