Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F50 I1 R1
|
300 |
54.7 |
1718999 |
98.4% |
1691495 |
149.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,627,887 |
(G)5→6 |
coding (1052/2244 nt) |
yfgF ← |
cyclic‑di‑GMP phosphodiesterase, anaerobic |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,627,882 | 1 | . | G | 97.9%
| 157.8
/ ‑4.0
| 47 | coding (1057/2244 nt) | yfgF | cyclic‑di‑GMP phosphodiesterase, anaerobic |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (27/19); total (28/19) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
CCGAAAGCTGATAAACATCTTCACCCGGTTCCAGCAAGGGTGACAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCA‑GGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCATATAATTGACAATAAAAGAGA > NC_000913/2627735‑2628025
|
ccGAAAGCTGATAAACATCTTCACCCGGTTCCAGCAAGGGTGACAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCA‑ggg < 2:55243/151‑1 (MQ=255)
gATAAACATCTTCACCCGGTTCCAGCAAGGGTGACAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCA‑ggggggcatc < 2:63661/149‑6 (MQ=255)
cATCTTCACCCGGTTCCAGCAAGGGTGACAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAAcg > 1:261387/1‑151 (MQ=255)
aTCTTCACCCGGTTCCAGCAAGGGTGACAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAAcgc > 1:231095/1‑151 (MQ=255)
ttCACCCGGTTCCAGCAAGGGTGACAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAAcgcgcg > 1:293768/1‑151 (MQ=255)
ttCACCCGGTTCCAGCAAGGGTGACAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAAcgcgcg > 1:53366/1‑151 (MQ=255)
cACCCGGTTCCAGCAAGGGTGACAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGAt < 2:382576/151‑1 (MQ=255)
aCCCGGTTCCAGCAAGGGTGACAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGAtt > 1:844074/1‑151 (MQ=255)
cGGTTCCAGCAAGGGTGACAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTc > 2:267055/1‑149 (MQ=255)
ggTTCCAGCAAGGGTGACAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAgg > 2:407117/1‑151 (MQ=255)
aaaGGGTGACAGTCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGTCATAGTTCTTAACCAGCATTTCCATGCAAGGGATGCGTAAATTACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGa > 1:534079/2‑149 (MQ=255)
gTGACAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTg > 2:446450/1‑151 (MQ=255)
aCAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCa < 2:397254/151‑1 (MQ=255)
ccAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGAt > 2:614963/1‑150 (MQ=255)
ccAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATg > 1:820739/1‑151 (MQ=255)
gaAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAaccacc < 2:844132/151‑1 (MQ=255)
gTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGt < 1:723708/151‑1 (MQ=255)
gCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACAt > 1:520925/1‑151 (MQ=255)
tCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCt > 2:117007/1‑151 (MQ=255)
cGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGa < 1:722224/151‑1 (MQ=255)
gcagcaTGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAACTAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGa > 2:349581/1‑150 (MQ=255)
agcaTGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATg > 1:686102/1‑151 (MQ=255)
agcaTGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATg > 2:138386/1‑151 (MQ=255)
atgatgCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGt < 2:730999/151‑1 (MQ=255)
gatgCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTc < 2:165188/150‑1 (MQ=255)
tCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCA‑GGGGGTCATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGctc > 1:508978/1‑151 (MQ=255)
ttAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGctctc > 2:487164/1‑151 (MQ=255)
tAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGctctct > 1:801695/1‑151 (MQ=255)
cAGCATTTCCAGGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACg > 1:359319/1‑151 (MQ=255)
gCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACggg < 1:759045/151‑1 (MQ=255)
cATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAg < 1:761217/151‑1 (MQ=255)
aTGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGt > 1:594778/1‑151 (MQ=255)
aTGCGTAAATAACAAGGCGCAGACCAGGGGGG‑CATCACGCACCGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCata < 1:36096/151‑1 (MQ=255)
aTGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCata < 2:446068/151‑1 (MQ=255)
aTGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCat > 2:206959/1‑150 (MQ=255)
tGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCatat > 1:33104/1‑151 (MQ=255)
gCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCAtata < 1:2461/151‑1 (MQ=255)
gTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCATATAAt < 2:50453/151‑1 (MQ=255)
aaCAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCTAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCATATAATTGACa < 2:743546/150‑1 (MQ=255)
aaCAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGCACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCATATAATTGACa > 1:32611/1‑150 (MQ=255)
aaCAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCTGTACAGCCATATAATTGACa < 2:262204/150‑1 (MQ=255)
aaaGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCATATAATTGACAATa < 1:691136/150‑1 (MQ=255)
aaGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCATATAATTGACAATaa > 1:53938/1‑150 (MQ=255)
gcgcAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCATATAATTGACAATaaaa < 2:492909/150‑1 (MQ=255)
gcAGACCAGGGGGG‑CATCACTCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCATATAATTGACAATAAAagag < 2:116716/151‑1 (MQ=255)
cggACCAGGGGGG‑CATCGCGCAGCGCGCAATTCAGGGCGCGAACATTGGCCAGAGGAACAACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGCCTGCCGCACTCCGACGGGTTGCGAGTGCAGCCATATAATTGACATTAAAaga < 1:832422/147‑1 (MQ=255)
cAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCATATAATTGACAATAAAag > 2:654464/1‑148 (MQ=255)
aGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCATATAATTGACAATAAAAgaga > 1:709454/1‑150 (MQ=255)
aGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCATATAATTGACAATAAAAgaga > 2:675613/1‑150 (MQ=255)
|
CCGAAAGCTGATAAACATCTTCACCCGGTTCCAGCAAGGGTGACAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCA‑GGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCATATAATTGACAATAAAAGAGA > NC_000913/2627735‑2628025
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
