Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,219,596	A→G	N35D (AAC→GAC)	patA →	putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,219,596	0	A	G	100.0%	174.0 / NA	49	N35D (AAC→GAC)	patA	putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/30); total (19/30)

CTTAAATCGGTATCATGTGATACGCGAGCCTCCGGAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAGGG  >  NC_000913/3219451‑3219735

cTTAAATCGGTATCATGTGATACGCGAGCCTCCGGAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCg                                                                                                                                         >  2:289955/1‑150 (MQ=255)

  tAAATCGGTATCATGTGATACGCGAGCCTCCGGAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCga                                                                                                                                        <  1:588767/149‑1 (MQ=255)

   aaaTCGGTATCATGTGATACGCGAGCCTCCGGAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGagag                                                                                                                                     <  2:183611/151‑1 (MQ=255)

           aTCATGTGATACGCGAGCCTCCGGAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTg                                                                                                                              >  1:373364/1‑150 (MQ=255)

                gtgATACGCGAGCCTCCGGAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATAc                                                                                                                         <  1:210144/150‑1 (MQ=255)

                gtgATACGCGAGCCTCCGGAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATAc                                                                                                                         <  1:412031/150‑1 (MQ=255)

                gtgATACGCGAGCCTCCGGAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACt                                                                                                                        <  2:37054/151‑1 (MQ=255)

                     aCGCGAGCCTCCGGAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCaa                                                                                                                    <  2:373388/150‑1 (MQ=255)

                     aCGCGAGCCTCCGGAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCaa                                                                                                                    >  1:766432/1‑150 (MQ=255)

                        cgAGCCTCCGGAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAagag                                                                                                                <  2:739251/151‑1 (MQ=255)

                        cgAGCCTCCGGAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAagag                                                                                                                <  2:625110/151‑1 (MQ=255)

                        cgAGCCTCCGGAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAagag                                                                                                                <  2:166713/151‑1 (MQ=255)

                        cgAGCCTCCGGAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAag                                                                                                                  >  1:401549/1‑149 (MQ=255)

                          aGCCTCCGGAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGc                                                                                                               >  2:89480/1‑150 (MQ=255)

                               ccGGAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTc                                                                                                          >  1:116979/1‑150 (MQ=255)

                               ccGGAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGACCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTc                                                                                                          <  1:282301/150‑1 (MQ=255)

                                 ggAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAAt                                                                                                       >  1:525010/1‑151 (MQ=255)

                                         ttttGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGtt                                                                                               >  2:254161/1‑151 (MQ=255)

                                                 aGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGt                                                                                       >  2:255614/1‑151 (MQ=255)

                                                      aCCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCg                                                                                   <  2:715191/150‑1 (MQ=255)

                                                         ttCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCaa                                                                                >  2:691583/1‑150 (MQ=255)

                                                             aGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCt                                                                            >  1:591346/1‑150 (MQ=255)

                                                                 cATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTAc                                                                       <  2:300858/151‑1 (MQ=255)

                                                                    cGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTAccg                                                                     <  2:636422/150‑1 (MQ=255)

                                                                                gCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGAtt                                                         <  2:853962/150‑1 (MQ=255)

                                                                                  aGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGTTGGATCATGAGGAGATGAAAGCGCTTGACCGAGAGGTGATTGAATCCTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCGGTTACCGCCGGCGGGGATTAc                                                       <  1:685366/150‑1 (MQ=255)

                                                                                  aGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTAc                                                       <  2:125487/150‑1 (MQ=255)

                                                                                  aGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTACg                                                      >  1:346583/1‑151 (MQ=255)

                                                                                   gcgcCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTACgg                                                     <  2:808569/151‑1 (MQ=255)

                                                                                       ccACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTACGGAGcc                                                 <  2:794341/151‑1 (MQ=255)

                                                                                        cACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTACGGAGCCg                                                <  2:329529/151‑1 (MQ=255)

                                                                                           gCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTACGGAGCCGt                                               >  2:722682/1‑149 (MQ=255)

                                                                                              cTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTACGGAGCCGTaga                                            >  1:390087/1‑149 (MQ=255)

                                                                                                gAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTACGGAGCCGTAGAGTgg                                        <  1:89457/151‑1 (MQ=255)

                                                                                                    ctcATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTACGGAGCCGTAGAGTGGCaa                                     <  2:780173/150‑1 (MQ=255)

                                                                                                       aTTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTACGGAGCCGTAGAGTGGCAAGCgg                                 <  1:686921/151‑1 (MQ=255)

                                                                                                         tGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTACGGAGCCGTAGAGTGGCAAGCggg                                <  2:138906/150‑1 (MQ=255)

                                                                                                           agaAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTACGGAGCCGTAGAGTGGCAAGCggg                                >  1:509218/1‑148 (MQ=255)

                                                                                                             aaGCGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGt                            <  1:92031/150‑1 (MQ=255)

                                                                                                                cGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTaa                        <  1:714173/151‑1 (MQ=255)

                                                                                                                cGAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTa                         <  2:33196/150‑1 (MQ=255)

                                                                                                                 gAACGCTGGATCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTaa                        >  1:545286/1‑150 (MQ=255)

                                                                                                                          aTCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGt              >  2:776002/1‑151 (MQ=255)

                                                                                                                           tCATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTc             >  2:243357/1‑151 (MQ=255)

                                                                                                                            cATGAGGAGATGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCg            >  2:527633/1‑151 (MQ=255)

                                                                                                                                agaggaTGAAAGCACTTGACTGGGAGGTGACTGAATGCTTCAAAGAACATGTGAGCCCGGGGTTTTTAGAGTACCGCAAGTCTTTTACCGCCGCCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAGATACCCTTGTCGaca         <  1:775590/146‑1 (MQ=255)

                                                                                                                                    gaTGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCAGGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAg    <  1:602898/151‑1 (MQ=255)

                                                                                                                                     aTGAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAgg   <  2:263284/151‑1 (MQ=255)

                                                                                                                                       gAAAGCACTTGACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAggg  <  2:546770/150‑1 (MQ=255)

CTTAAATCGGTATCATGTGATACGCGAGCCTCCGGAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAGGG  >  NC_000913/3219451‑3219735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A