Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,227,104	G→A	V291M (GTG→ATG)	ygjI →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,227,104	0	G	A	100.0%	174.2 / NA	49	V291M (GTG→ATG)	ygjI	putative transporter
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/27); total (22/27)

GTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAG  >  NC_000913/3226962‑3227242

gTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCtt                                                                                                                                     <  2:36383/150‑1 (MQ=255)

      cGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACcgcg                                                                                                                              >  1:150899/1‑151 (MQ=255)

      cGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACcgcg                                                                                                                              >  1:217363/1‑151 (MQ=255)

           cctcGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGt                                                                                                                          <  2:372099/150‑1 (MQ=255)

                  gCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTcc                                                                                                                  <  2:590304/151‑1 (MQ=255)

                      aTTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCctgc                                                                                                              <  2:733599/151‑1 (MQ=255)

                          aCGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGAATTCCACGGCATGGCGGCGTATTTTGGTCTAACGGAAGCGTTGATGAATCGCTTTGCCGGTCTGATGTCCTTTACCGCGATGTTCGGTTTCCTGCTGAt                                                                                                          >  2:769564/1‑151 (MQ=255)

                             tCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGAtgtg                                                                                                       >  2:754019/1‑151 (MQ=255)

                                 cGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGAcc                                                                                                   <  1:507768/151‑1 (MQ=255)

                                    cagcagCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGc                                                                                                 <  2:852733/150‑1 (MQ=255)

                                     agcagcAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTATGATTACCTTTACCGCGATGTTCGGGTCCCTGCTGCTGTGGACCGCa                                                                                                >  2:628621/1‑150 (MQ=255)

                                              gagTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGa                                                                                      <  1:500799/151‑1 (MQ=255)

                                                   gAAATTTACCGGCGGATCGGTGCAGGAATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAAtt                                                                                 >  2:439973/1‑151 (MQ=255)

                                                     aaTTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAAtttt                                                                               >  1:62607/1‑151 (MQ=255)

                                                        ttACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTttct                                                                             <  2:750800/150‑1 (MQ=255)

                                                             ggcggATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCg                                                                       <  1:687927/151‑1 (MQ=255)

                                                                     ggTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCg                                                               >  1:626990/1‑151 (MQ=255)

                                                                     ggTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCg                                                               <  1:855711/151‑1 (MQ=255)

                                                                       tGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCgg                                                              >  2:755936/1‑150 (MQ=255)

                                                                             tATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCAt                                                       >  2:26981/1‑151 (MQ=255)

                                                                                tCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCtt                                                    >  1:115018/1‑151 (MQ=255)

                                                                                      gCATGGCGGCGTATTTTAGTCTACCGGAAGCATTGACGAATCGCTTTATCGCTCCAATGTCCTTTTCCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCACCGACAGTGAAAATTTTCTGCTCCGAAATCCCGGTAGGCATCTTAGGTa                                               <  1:235145/150‑1 (MQ=255)

                                                                                        aTGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTa                                               >  2:627688/1‑148 (MQ=255)

                                                                                        aTGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGa                                            <  2:568318/151‑1 (MQ=255)

                                                                                           gcggcgTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGaaaa                                         <  2:150922/151‑1 (MQ=255)

                                                                                           gcggcgTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGaaa                                          >  1:458544/1‑150 (MQ=255)

                                                                                            cggcgTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAAc                                        <  2:738652/151‑1 (MQ=255)

                                                                                             ggcgTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAAcc                                       <  2:796679/151‑1 (MQ=255)

                                                                                              gcgTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCg                                      <  1:155831/151‑1 (MQ=255)

                                                                                               cgTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGTTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGt                                     >  2:435569/1‑151 (MQ=255)

                                                                                               cgTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGt                                     <  2:579764/151‑1 (MQ=255)

                                                                                                    tttGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAAc                                >  1:14687/1‑151 (MQ=255)

                                                                                                        gTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGaa                            <  2:728561/151‑1 (MQ=255)

                                                                                                        gTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGa                             >  2:655745/1‑150 (MQ=255)

                                                                                                            aCCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACg                          <  2:707725/149‑1 (MQ=255)

                                                                                                            aCCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGa                         <  2:559835/150‑1 (MQ=255)

                                                                                                             ccGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACAGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTTAACGaa                        >  1:648780/1‑150 (MQ=255)

                                                                                                              cGGAAGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGaaaa                      <  1:97170/151‑1 (MQ=255)

                                                                                                                 aaGCGTTGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACgg                   >  1:585044/1‑151 (MQ=255)

                                                                                                                      ttGATGAATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTc              >  1:33013/1‑151 (MQ=255)

                                                                                                                       tgatgaATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTc              <  1:608855/150‑1 (MQ=255)

                                                                                                                         atgaATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCgg           <  2:694785/151‑1 (MQ=255)

                                                                                                                         atgaATCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCgg           >  1:489354/1‑151 (MQ=255)

                                                                                                                            aaTCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGgcgc        <  2:190164/151‑1 (MQ=255)

                                                                                                                            aaTCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGgcg         >  1:135383/1‑150 (MQ=255)

                                                                                                                             aTCGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGgcgcg       <  1:810417/151‑1 (MQ=255)

                                                                                                                               cGCTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGgcgcgcg     >  1:666565/1‑151 (MQ=255)

                                                                                                                                 cTTTGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGgcgcgcgc    <  2:338624/150‑1 (MQ=255)

                                                                                                                                   ttGTCGGTCTGATGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAg  <  2:458580/150‑1 (MQ=255)

GTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAG  >  NC_000913/3226962‑3227242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A