Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	14	56.9	1764756	99.2%	1750637	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	962,056	G→T	G21V (GGT→GTT)	rpsA →	30S ribosomal subunit protein S1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	962,056	0	G	T	100.0%	203.1 / NA	54	G21V (GGT→GTT)	rpsA	30S ribosomal subunit protein S1
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (30/24); total (30/24)

TTGCAATGGAATGACAGCGGGTATGTTAAACAACCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGGTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGAC  >  NC_000913/961909‑962201

ttGCAATGGAATGACAGCGGGTATGTTAAACAACCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTc                                                                                                                                                >  2:834756/1‑151 (MQ=255)

   cAATGGAATGACAGCGGGTATGTTAAACAACCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTAt                                                                                                                                             >  1:475746/1‑151 (MQ=255)

        gAATGACAGCGGGTATGTTAAACAACCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTAtcgttc                                                                                                                                        <  1:763390/151‑1 (MQ=255)

         aaTGACAGCGGGTATGTTAAACAACCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTAtcgttcg                                                                                                                                       >  2:814104/1‑151 (MQ=255)

         aaTGACAGCGGGCATGTTAAACAACCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAAACTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTAtcgttcg                                                                                                                                       >  2:376330/1‑151 (MQ=255)

          aTGACAGCGGGTATGTTAAACAACCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGGGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTAtcgttcgt                                                                                                                                      <  1:386443/151‑1 (MQ=255)

          aTGACAGCGGGTATGTTAAACAACCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTAtcgttcgt                                                                                                                                      <  1:355308/151‑1 (MQ=255)

               aGCGGGTATGTTAAACAACCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCgt                                                                                                                                 >  2:692141/1‑151 (MQ=255)

                  gggTATGTTAAACAACCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCgttgt                                                                                                                              <  2:582175/151‑1 (MQ=255)

                           aaaCAACCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTAt                                                                                                                     >  1:283004/1‑151 (MQ=255)

                            aacaacCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATc                                                                                                                    >  2:507722/1‑151 (MQ=255)

                            aacaacCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATc                                                                                                                    >  1:373201/1‑151 (MQ=255)

                            aacaacCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATc                                                                                                                    <  1:661936/151‑1 (MQ=255)

                            aacaacCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATc                                                                                                                    <  1:487796/151‑1 (MQ=255)

                              caacCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGa                                                                                                                  <  2:445479/151‑1 (MQ=255)

                                     aTCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGAc                                                                                                           >  2:785371/1‑151 (MQ=255)

                                               gAGCCAGGTGGACGTTAAAAATAAACCTGAAGATTAAACATTACAGAATCTTTTGCTCAACTTTTTGAAGAGTCCTTAAAAGAAACCGACACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTgg                                                                                                 <  2:563541/151‑1 (MQ=255)

                                                    aGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGAc                                                                                            <  1:381845/151‑1 (MQ=255)

                                                     ggTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACg                                                                                           <  2:70937/151‑1 (MQ=255)

                                                        ggACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTg                                                                                        >  1:433984/1‑151 (MQ=255)

                                                         gACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTgg                                                                                       >  2:366169/1‑151 (MQ=255)

                                                                 atatAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAAt                                                                               <  1:423221/151‑1 (MQ=255)

                                                                       aCCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGt                                                                         >  2:514545/1‑151 (MQ=255)

                                                                         cTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTcc                                                                       >  2:472472/1‑151 (MQ=255)

                                                                            aaGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGcc                                                                    >  1:776936/1‑151 (MQ=255)

                                                                             aGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCa                                                                   <  2:341361/151‑1 (MQ=255)

                                                                                 tAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATccc                                                               >  2:750462/1‑151 (MQ=255)

                                                                                  aaaCATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATccc                                                               <  1:395799/150‑1 (MQ=255)

                                                                                     cATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCt                                                           >  2:694923/1‑151 (MQ=255)

                                                                                      aTGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTg                                                          <  2:44092/151‑1 (MQ=255)

                                                                                        gACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGa                                                         <  1:365862/150‑1 (MQ=255)

                                                                                             aaTCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGtt                                                   >  2:499274/1‑151 (MQ=255)

                                                                                                   ttGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCaaaa                                              <  1:252881/150‑1 (MQ=255)

                                                                                                    tGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAAc                                            >  1:408076/1‑151 (MQ=255)

                                                                                                        cAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGccc                                        >  2:774372/1‑151 (MQ=255)

                                                                                                         aaCTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGccc                                        >  1:398179/1‑150 (MQ=255)

                                                                                                          aCTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAg                                      <  2:553309/151‑1 (MQ=255)

                                                                                                           ctctTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAgg                                     >  2:20762/1‑151 (MQ=255)

                                                                                                             ctTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAgg                                     <  1:69155/149‑1 (MQ=255)

                                                                                                                 gAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGc                               <  2:270942/151‑1 (MQ=255)

                                                                                                                     agTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGAAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTgg                            >  1:583312/1‑150 (MQ=255)

                                                                                                                      gTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGaa                          <  2:123001/151‑1 (MQ=255)

                                                                                                                        ccTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAAt                        >  1:161199/1‑151 (MQ=255)

                                                                                                                        ccTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAAt                        >  1:383669/1‑151 (MQ=255)

                                                                                                                            aaaaGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATcc                      >  1:833486/1‑149 (MQ=255)

                                                                                                                            aaaaGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCag                    <  2:783116/151‑1 (MQ=255)

                                                                                                                                 aaaTCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCaggtagg               <  1:157639/151‑1 (MQ=255)

                                                                                                                                      gAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACg          >  2:747301/1‑151 (MQ=255)

                                                                                                                                       aaaCCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGa         <  2:205280/151‑1 (MQ=255)

                                                                                                                                           ccgcccgGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGtt     >  1:87197/1‑151 (MQ=255)

                                                                                                                                             gcccgGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTg    >  2:90710/1‑150 (MQ=255)

                                                                                                                                              cccgGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTg    >  1:713449/1‑149 (MQ=255)

                                                                                                                                              cccgGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGAc  >  2:220323/1‑151 (MQ=255)

                                                                                                                                              cccgGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGAc  <  1:773835/151‑1 (MQ=255)

TTGCAATGGAATGACAGCGGGTATGTTAAACAACCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGGTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGAC  >  NC_000913/961909‑962201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A