Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I1 R1	14	47.9	1492993	99.0%	1478063	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,347,775	C→A	E380* (GAG→TAG)	rnb ←	ribonuclease II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,347,775	0	C	A	100.0%	212.2 / NA	54	E380* (GAG→TAG)	rnb	ribonuclease II
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (24/30); total (24/30)

TGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTCGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCT  >  NC_000913/1347629‑1347920

tGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGcg                                                                                                                                               >  2:665903/1‑151 (MQ=255)

 gCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGcg                                                                                                                                               <  1:152843/150‑1 (MQ=255)

  ccAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGcgac                                                                                                                                             <  2:605607/151‑1 (MQ=255)

     gcgcGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGat                                                                                                                                          >  2:132457/1‑151 (MQ=255)

        cgTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATc                                                                                                                                       <  1:421139/151‑1 (MQ=255)

          tCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATcc                                                                                                                                      >  2:148553/1‑150 (MQ=255)

           cGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCa                                                                                                                                     <  2:209953/150‑1 (MQ=255)

           cGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAg                                                                                                                                    <  2:83024/151‑1 (MQ=255)

               aTTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGTGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACt                                                                                                                                <  1:88438/151‑1 (MQ=255)

                       gATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCttt                                                                                                                        >  1:16447/1‑151 (MQ=255)

                         tCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCttttt                                                                                                                      >  1:217511/1‑151 (MQ=255)

                          cAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCttttt                                                                                                                      <  2:95704/150‑1 (MQ=255)

                          cAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTc                                                                                                                     >  2:565259/1‑151 (MQ=255)

                          cAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTc                                                                                                                     >  2:489989/1‑151 (MQ=255)

                                ccATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCgag                                                                                                               <  1:85731/151‑1 (MQ=255)

                                 cATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCgaga                                                                                                              <  1:34747/151‑1 (MQ=255)

                                   tatGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGaa                                                                                                             >  2:142834/1‑150 (MQ=255)

                                   tatGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAAt                                                                                                            >  2:307960/1‑151 (MQ=255)

                                      gCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATaaa                                                                                                         <  2:274265/151‑1 (MQ=255)

                                      gCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATaaa                                                                                                         <  1:32977/151‑1 (MQ=255)

                                      gCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATaa                                                                                                          >  2:731269/1‑150 (MQ=255)

                                          gTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCgg                                                                                                     >  1:372789/1‑151 (MQ=255)

                                           ttATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGt                                                                                                    >  2:731573/1‑151 (MQ=255)

                                               aGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAAt                                                                                                 <  1:44876/150‑1 (MQ=255)

                                               aGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAAt                                                                                                 <  1:661813/150‑1 (MQ=255)

                                               aGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAAt                                                                                                 <  2:311477/150‑1 (MQ=255)

                                               aGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATc                                                                                                >  1:205985/1‑151 (MQ=255)

                                                   gCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCggg                                                                                            <  2:595848/151‑1 (MQ=255)

                                                   gCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCgg                                                                                             <  1:244678/150‑1 (MQ=255)

                                                           cGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCttt                                                                                    <  2:273259/151‑1 (MQ=255)

                                                                        cAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCg                                                                       >  1:108012/1‑151 (MQ=255)

                                                                                     gCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGcc                                                          >  1:13209/1‑151 (MQ=255)

                                                                                      cacaAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGcc                                                          >  2:254389/1‑150 (MQ=255)

                                                                                       acaAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCAc                                                        <  1:303959/151‑1 (MQ=255)

                                                                                         aaaTGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTc                                                      <  2:129176/151‑1 (MQ=255)

                                                                                          aaTGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTc                                                      >  2:144507/1‑150 (MQ=255)

                                                                                           aTGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTcgc                                                    <  2:371304/151‑1 (MQ=255)

                                                                                             gTTAGCGGCAATCATCGCTTCCTCGTCGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCCCTTCTCCTTTTTCCCCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGATTATGACGCATCTcgcc                                                   <  2:76666/150‑1 (MQ=255)

                                                                                                    gCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGtt                                           <  2:523927/151‑1 (MQ=255)

                                                                                                     cAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTg                                          <  1:389456/151‑1 (MQ=255)

                                                                                                      aaTCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTgg                                         <  1:231078/151‑1 (MQ=255)

                                                                                                          atcGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCaa                                      >  2:214625/1‑150 (MQ=255)

                                                                                                            cGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAAtt                                   >  2:591965/1‑151 (MQ=255)

                                                                                                            cGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAAt                                    <  1:132464/150‑1 (MQ=255)

                                                                                                                  ttcGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCt                             >  2:102589/1‑151 (MQ=255)

                                                                                                                   tcGACGATACGGTTGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCt                             >  2:659753/1‑150 (MQ=255)

                                                                                                                                tGGCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACtt                <  1:356753/150‑1 (MQ=255)

                                                                                                                                  gCAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGtt             >  1:338639/1‑151 (MQ=255)

                                                                                                                                   cAATGCGACGAGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGTTc            >  1:203834/1‑151 (MQ=255)

                                                                                                                                           cgaGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTg    <  1:427274/151‑1 (MQ=255)

                                                                                                                                           cgaGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTg    <  2:157935/151‑1 (MQ=255)

                                                                                                                                           cgaGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGTTCCGCAATTg    <  2:502181/151‑1 (MQ=255)

                                                                                                                                            gaGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGc   <  2:593670/151‑1 (MQ=255)

                                                                                                                                             aGGCTAGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATCAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCt  >  2:647179/1‑151 (MQ=255)

TGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTCGGCGACGATATCCAGCACTTCACCTTTTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCT  >  NC_000913/1347629‑1347920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A