Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F50 I2 R1 173 150.8 4954114 96.5% 4780720 150.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,453,207 T→C T22A (ACC→GCC)  rpsJ ← 30S ribosomal subunit protein S10

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,453,2070TC98.9% 659.2 / ‑2.1 177T22A (ACC→GCC) rpsJ30S ribosomal subunit protein S10
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (84/91);  total (85/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01

Only 100 of 177 total aligned reads displayed.

AAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATGGGAGAGTGTAACCGTTCTT  >  NC_000913/3453059‑3453354
                                                                                                                                                    |                                                                                                                                                   
   tGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCtt                                                                                                                                                >  4:1904029/1‑151 (MQ=255)
    gAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCtt                                                                                                                                                <  4:478553/150‑1 (MQ=255)
    gAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTg                                                                                                                                               <  1:1327157/151‑1 (MQ=255)
     aGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTg                                                                                                                                               >  4:674358/1‑150 (MQ=255)
     aGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTg                                                                                                                                               >  2:258819/1‑150 (MQ=255)
       tACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTgatc                                                                                                                                            >  1:277785/1‑151 (MQ=255)
          ggATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTgatcgat                                                                                                                                         >  4:1580752/1‑151 (MQ=255)
          ggATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTgatcgat                                                                                                                                         >  4:1097214/1‑151 (MQ=255)
          ggATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTgatcgat                                                                                                                                         >  1:1451237/1‑151 (MQ=255)
              ttCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTgatcgatccg                                                                                                                                      >  4:1631591/1‑148 (MQ=255)
                 gTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACga                                                                                                                                  <  1:1035523/151‑1 (MQ=255)
                   aCTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACgatg                                                                                                                                <  2:83671/151‑1 (MQ=255)
                   aCTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACgat                                                                                                                                 <  1:1050764/150‑1 (MQ=255)
                      gATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACgatgat                                                                                                                              <  4:1244981/150‑1 (MQ=255)
                      gATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACgatgat                                                                                                                              <  3:298823/150‑1 (MQ=255)
                      gATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACgatga                                                                                                                               <  2:217964/149‑1 (MQ=255)
                       aTCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCa                                                                                                                            <  1:506349/151‑1 (MQ=255)
                         cgcgcgcgTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCaa                                                                                                                           <  1:1563915/150‑1 (MQ=255)
                              gcgTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCtt                                                                                                                     <  1:193525/151‑1 (MQ=255)
                               cgTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCttt                                                                                                                    <  4:1847570/151‑1 (MQ=255)
                                gTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCttt                                                                                                                    <  2:539839/150‑1 (MQ=255)
                                 tCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTc                                                                                                                   >  3:235105/1‑150 (MQ=255)
                                    ttgttgACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGc                                                                                                               >  4:875079/1‑151 (MQ=255)
                                      gttgACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCgg                                                                                                             <  4:1699522/151‑1 (MQ=255)
                                        tgACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGa                                                                                                            <  1:37952/150‑1 (MQ=255)
                                         gACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGAt                                                                                                           <  4:1496467/150‑1 (MQ=255)
                                         gACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATa                                                                                                          <  2:242476/151‑1 (MQ=255)
                                            gTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACgg                                                                                                       >  2:275514/1‑151 (MQ=255)
                                              gCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGAt                                                                                                     <  4:26532/151‑1 (MQ=255)
                                               cGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGAtt                                                                                                    >  4:548234/1‑151 (MQ=255)
                                               cGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGAtt                                                                                                    <  1:1199128/151‑1 (MQ=255)
                                               cGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGAtt                                                                                                    >  1:1142485/1‑151 (MQ=255)
                                                ggggAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGAtt                                                                                                    <  1:1708938/150‑1 (MQ=255)
                                                cgggAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTc                                                                                                   >  3:482255/2‑151 (MQ=255)
                                                     gaTCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTgg                                                                                              >  3:247059/1‑151 (MQ=255)
                                                       tCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGtt                                                                                            >  1:1178575/1‑151 (MQ=255)
                                                       tCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGtt                                                                                            >  4:525283/1‑151 (MQ=255)
                                                          gAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTg                                                                                         >  2:233909/1‑151 (MQ=255)
                                                          gAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTg                                                                                         <  1:485482/151‑1 (MQ=255)
                                                          gAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTg                                                                                         >  4:897810/1‑151 (MQ=255)
                                                             cAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCAt                                                                                      >  3:503252/1‑151 (MQ=255)
                                                                tGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATg                                                                                     >  2:42630/1‑149 (MQ=255)
                                                                 gAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATgag                                                                                   <  4:1211486/150‑1 (MQ=255)
                                                                      gcTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCaga                                                                             <  4:660615/151‑1 (MQ=255)
                                                                      gcTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCaga                                                                             <  1:867762/151‑1 (MQ=255)
                                                                           ttGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTcc                                                                        >  1:1627706/1‑151 (MQ=255)
                                                                            tGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTcc                                                                        >  1:633236/1‑150 (MQ=255)
                                                                             gCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCaa                                                                      <  4:304037/151‑1 (MQ=255)
                                                                             gCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCa                                                                       <  4:82437/150‑1 (MQ=255)
                                                                             gCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCa                                                                       <  4:1018050/150‑1 (MQ=255)
                                                                              cGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAat                                                                     <  1:59624/151‑1 (MQ=255)
                                                                              cGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAat                                                                     <  4:627560/151‑1 (MQ=255)
                                                                               gtgtCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAatt                                                                    <  2:374035/151‑1 (MQ=255)
                                                                                 gtCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAatta                                                                   <  4:188444/150‑1 (MQ=255)
                                                                                  tCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAattatt                                                                 >  4:150025/1‑151 (MQ=255)
                                                                                   cGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTAttt                                                                >  1:186848/1‑151 (MQ=255)
                                                                                      cAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTtat                                                             <  1:771437/151‑1 (MQ=255)
                                                                                         cGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATaa                                                           >  4:222789/1‑150 (MQ=255)
                                                                                           ggATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACg                                                        <  4:1131872/151‑1 (MQ=255)
                                                                                           ggATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACg                                                        <  4:1285271/151‑1 (MQ=255)
                                                                                           ggATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACg                                                        >  4:1452136/1‑151 (MQ=255)
                                                                                            gATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGa                                                       <  1:1194134/151‑1 (MQ=255)
                                                                                            gATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGa                                                       <  4:343900/151‑1 (MQ=255)
                                                                                             aTCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGa                                                       >  1:987074/1‑150 (MQ=255)
                                                                                              tCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGaa                                                      >  1:734427/1‑150 (MQ=255)
                                                                                                 gACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATg                                                  >  4:262351/1‑151 (MQ=255)
                                                                                                   ccACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGa                                                 <  1:571957/150‑1 (MQ=255)
                                                                                                   ccACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGAt                                                >  4:964518/1‑151 (MQ=255)
                                                                                                    cACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGAtt                                               >  2:418775/1‑151 (MQ=255)
                                                                                                     aCGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGAtt                                               >  1:944688/1‑150 (MQ=255)
                                                                                                     aCGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTa                                              <  1:520404/151‑1 (MQ=255)
                                                                                                      cGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTa                                              <  1:1161838/150‑1 (MQ=255)
                                                                                                      cGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTAc                                             >  4:1532740/1‑151 (MQ=255)
                                                                                                      cGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTAc                                             >  1:142376/1‑151 (MQ=255)
                                                                                                       ggACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTAct                                            >  3:427390/1‑151 (MQ=255)
                                                                                                        gACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTActc                                           >  1:241904/1‑151 (MQ=255)
                                                                                                         aCCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTActcc                                          <  1:1648031/151‑1 (MQ=255)
                                                                                                           cTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTActcctc                                        >  4:835922/1‑151 (MQ=255)
                                                                                                              cgcACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGa                                     <  2:292423/151‑1 (MQ=255)
                                                                                                               gcACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGAc                                    >  3:367862/1‑151 (MQ=255)
                                                                                                                 aCCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGAccc                                  >  1:205028/1‑151 (MQ=255)
                                                                                                                    aGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCAtt                               >  2:32933/1‑151 (MQ=255)
                                                                                                                     gTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTa                              <  4:1801123/151‑1 (MQ=255)
                                                                                                                     gTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTa                              <  4:1725483/151‑1 (MQ=255)
                                                                                                                       gcgcTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTAc                             <  1:1697568/150‑1 (MQ=255)
                                                                                                                       gcgcTTGGCAGTCACGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTAc                             <  1:805051/150‑1 (MQ=255)
                                                                                                                         gcTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGAt                          >  2:189680/1‑151 (MQ=255)
                                                                                                                            tGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGaaa                                                     >  1:174302/1‑121 (MQ=255)
                                                                                                                                 gTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATggg                   >  4:580793/1‑150 (MQ=255)
                                                                                                                                 gTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATGGga                  <  3:210796/151‑1 (MQ=255)
                                                                                                                                     cgacgaTTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATGGGAGAgt              <  4:1034045/151‑1 (MQ=255)
                                                                                                                                     cgacgaTTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATGGGAGAgt              <  3:182023/151‑1 (MQ=255)
                                                                                                                                     cgacgaTTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATGGGAGAgt              <  4:1737381/151‑1 (MQ=255)
                                                                                                                                      gacgaTTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATGGGAGAgtg             >  4:405821/1‑151 (MQ=255)
                                                                                                                                      gacgaTTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATGGGAGAgtg             >  1:1305254/1‑151 (MQ=255)
                                                                                                                                      gacgaTTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATGGGAGAgtg             <  1:1181642/151‑1 (MQ=255)
                                                                                                                                      gacgaTTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATGGGAGAgtg             >  1:1500199/1‑151 (MQ=255)
                                                                                                                                       acgaTTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATGGGAGAgtgt            >  4:1348960/1‑151 (MQ=255)
                                                                                                                                              ccGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATGGGAGAGTGTAACCGtt     >  4:458645/1‑151 (MQ=255)
                                                                                                                                               cgcgGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATGGGAGAGTGTAACCGTTc    <  1:1226283/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATGGGAGAGTGTAACCGTTCTT  >  NC_000913/3453059‑3453354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A