Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,453,207	T→C	T22A (ACC→GCC)	rpsJ ←	30S ribosomal subunit protein S10

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,453,207	0	T	C	95.5%	148.1 / ‑1.4	44	T22A (ACC→GCC)	rpsJ	30S ribosomal subunit protein S10
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (19/23); total (20/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

GGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATGGGAGAGTGTAACCGT  >  NC_000913/3453069‑3453350

ggATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTgatcgat                                                                                                                                     >  2:44614/1‑151 (MQ=255)

        tACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACgat                                                                                                                             >  2:586918/1‑151 (MQ=255)

         aCTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACgatg                                                                                                                            <  1:308825/151‑1 (MQ=255)

         aCTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACgatg                                                                                                                            >  1:39194/1‑151 (MQ=255)

             aTCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATc                                                                                                                         >  2:117959/1‑150 (MQ=255)

                          ttgttgACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCa                                                                                                              <  1:476128/148‑1 (MQ=255)

                          ttgttgACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAgg                                                                                                            <  2:328799/150‑1 (MQ=255)

                          ttgttgACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAg                                                                                                             <  1:315850/149‑1 (MQ=255)

                              tgACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGAt                                                                                                       >  2:310178/1‑151 (MQ=255)

                                aCGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATAc                                                                                                     <  1:475432/151‑1 (MQ=255)

                                           gaTCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTgg                                                                                          >  2:519364/1‑151 (MQ=255)

                                               ggAACAGTGAAGGGCTCTTTGCGGGTCGGCAGCGGGGTCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAAGCGGCTACGGATTCTTTGGTTCt                                                                                      >  2:532472/2‑151 (MQ=255)

                                               agcaCAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGTATCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCt                                                                                      <  2:165886/148‑1 (MQ=255)

                                                 aaCAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGc                                                                                    <  2:194354/151‑1 (MQ=255)

                                                  aCAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGc                                                                                    >  1:333745/1‑150 (MQ=255)

                                                   cAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCAt                                                                                  <  1:50939/151‑1 (MQ=255)

                                                    aGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATg                                                                                 >  2:404665/1‑151 (MQ=255)

                                                       gAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATgaga                                                                              <  2:240083/151‑1 (MQ=255)

                                                              tctTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGc                                                                       >  1:332176/1‑151 (MQ=255)

                                                               ctTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCt                                                                      >  1:239334/1‑151 (MQ=255)

                                                                tttGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTc                                                                     >  1:446649/1‑151 (MQ=255)

                                                                 ttGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGc                                                                       <  2:277098/148‑1 (MQ=255)

                                                                  tGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTcc                                                                    >  2:165102/1‑150 (MQ=255)

                                                                         cGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTAttt                                                            <  2:531092/151‑1 (MQ=255)

                                                                         cGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTAttt                                                            <  2:598762/151‑1 (MQ=255)

                                                                              gCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATaa                                                       <  2:611752/151‑1 (MQ=255)

                                                                              gCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATaa                                                       <  2:69390/151‑1 (MQ=255)

                                                                                   aTCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGa                                                   >  2:205288/1‑150 (MQ=255)

                                                                                      ggACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAAt                                               <  1:597235/151‑1 (MQ=255)

                                                                                        aCCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGa                                             <  2:384681/151‑1 (MQ=255)

                                                                                           aCGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTa                                          <  2:332169/151‑1 (MQ=255)

                                                                                             ggACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTAct                                        <  2:164681/151‑1 (MQ=255)

                                                                                                     gcACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGAc                                <  2:632090/151‑1 (MQ=255)

                                                                                                      cACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGAATGAGACCAGAGCTCCAATTATTTTATAAACAAAAATGATTACTCCTCAGAcc                               >  2:356305/1‑151 (MQ=255)

                                                                                                            tGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGAccc                              <  2:283017/146‑1 (MQ=255)

                                                                                                            tGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGAccc                              >  1:283024/1‑146 (MQ=255)

                                                                                                            tGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTAc                         >  1:549764/1‑151 (MQ=255)

                                                                                                                   ggCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGa                   <  1:412204/150‑1 (MQ=255)

                                                                                                                   ggCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGAt                  <  2:27940/151‑1 (MQ=255)

                                                                                                                    gCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATg                 >  1:366760/1‑151 (MQ=255)

                                                                                                                      aGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATggg               >  1:124142/1‑151 (MQ=255)

                                                                                                                          tcGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATGGgag             >  2:237502/1‑149 (MQ=255)

                                                                                                                           cgacgaTTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATGGgag             <  1:169174/148‑1 (MQ=255)

                                                                                                                                    ccGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATGGGAGAGTGTAACCGt  <  1:165107/150‑1 (MQ=255)

GGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATGGGAGAGTGTAACCGT  >  NC_000913/3453069‑3453350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A