Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,109,893	C→T	R187C (CGC→TGC)	opgG →	OPG biosynthetic periplasmic beta‑1,6 branching glycosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,109,893	0	C	T	97.9%	184.6 / ‑3.2	48	R187C (CGC→TGC)	opgG	OPG biosynthetic periplasmic beta‑1,6 branching glycosyltransferase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (30/17); minor base A (0/1); total (30/18)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

CCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAGTCGA  >  NC_000913/1109746‑1110037

ccGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCt                                                                                                                                               >  1:59962/1‑151 (MQ=255)

    tCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCa                                                                                                                                            >  1:24906/1‑150 (MQ=255)

            aaaGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCt                                                                                                                                   >  2:721999/1‑151 (MQ=255)

             aaGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTg                                                                                                                                  <  1:543465/151‑1 (MQ=255)

              aGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTgg                                                                                                                                 >  1:374976/1‑151 (MQ=255)

                     aaCGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGc                                                                                                                          <  2:620157/151‑1 (MQ=255)

                           gAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCa                                                                                                                     >  2:15334/1‑150 (MQ=255)

                              aTCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAAc                                                                                                                 >  1:536659/1‑151 (MQ=255)

                               tCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAAcc                                                                                                                >  1:433942/1‑151 (MQ=255)

                                 gTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGa                                                                                                              <  1:723353/151‑1 (MQ=255)

                                  tCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGAc                                                                                                             >  1:692186/1‑151 (MQ=255)

                                  tCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGAc                                                                                                             >  2:51631/1‑151 (MQ=255)

                                            cGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACg                                                                                                    <  1:431063/150‑1 (MQ=255)

                                                         ttCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATg                                                                                      >  1:247/1‑151 (MQ=255)

                                                              cgTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTg                                                                                 >  1:493498/1‑151 (MQ=255)

                                                              cgTGATTGGTGCAGGGCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTg                                                                                 >  2:332708/1‑151 (MQ=255)

                                                                tGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCt                                                                               >  1:21238/1‑151 (MQ=255)

                                                                    tGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGa                                                                            <  2:534065/150‑1 (MQ=255)

                                                                      gTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTc                                                                         >  1:177792/1‑151 (MQ=255)

                                                                        gCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGAGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTcgc                                                                       <  2:254408/151‑1 (MQ=255)

                                                                           ggtcaggtTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTcgccgc                                                                    <  1:190700/151‑1 (MQ=255)

                                                                                ggtTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCg                                                               <  1:398548/151‑1 (MQ=255)

                                                                                 gtTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCg                                                               >  1:641644/1‑150 (MQ=255)

                                                                                  tttATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGAc                                                             >  1:275768/1‑151 (MQ=255)

                                                                                    tATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGAc                                                             >  2:592857/1‑149 (MQ=255)

                                                                                    tATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGAc                                                             >  2:155696/1‑149 (MQ=255)

                                                                                    tATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACa                                                            <  2:521234/150‑1 (MQ=255)

                                                                                      tGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAgg                                                          >  2:668470/1‑150 (MQ=255)

                                                                                         ccTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCt                                                      >  2:91412/1‑151 (MQ=255)

                                                                                             tCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTAc                                                   >  2:296604/1‑150 (MQ=255)

                                                                                             tCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTAc                                                   <  1:507655/150‑1 (MQ=255)

                                                                                                gCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACa                                                  <  2:653950/148‑1 (MQ=255)

                                                                                                gCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAAt                                               <  1:625870/151‑1 (MQ=255)

                                                                                                gCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAAt                                               >  1:442061/1‑151 (MQ=255)

                                                                                                    gcgGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGt                                           >  1:163904/1‑151 (MQ=255)

                                                                                                     cgGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTg                                          <  2:746401/151‑1 (MQ=255)

                                                                                                      ggCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTgg                                         >  2:617648/1‑151 (MQ=255)

                                                                                                         cTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGtt                                       >  2:65280/1‑150 (MQ=255)

                                                                                                          tGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTAt                                     >  1:391202/1‑151 (MQ=255)

                                                                                                              aaTTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGcc                                  >  1:304383/1‑150 (MQ=255)

                                                                                                              aaTTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCa                                 <  1:342698/151‑1 (MQ=255)

                                                                                                               aTTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGcc                                  >  2:370946/1‑149 (MQ=255)

                                                                                                                            tGCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGacaca                     <  2:60780/149‑2 (MQ=255)

                                                                                                                             gCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGtt                  <  1:25990/151‑1 (MQ=255)

                                                                                                                             gCCATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGt                   <  2:640912/150‑1 (MQ=255)

                                                                                                                               cATCGGGTGAAGAATTTCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTtgt                <  1:741280/151‑1 (MQ=255)

                                                                                                                                              ttCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAGTCGa  >  2:618567/1‑150 (MQ=255)

                                                                                                                                              ttCCGTGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAGTCGa  >  1:104625/1‑150 (MQ=255)

CCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAGTCGA  >  NC_000913/1109746‑1110037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A