Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,704,016	A→G	T16T (ACT→ACC)	rnc ←	RNase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,704,016	0	A	G	100.0%	118.7 / NA	32	T16T (ACT→ACC)	rnc	RNase III
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (3/29); total (3/29)

CATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAAGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGACCTGATATACATGGTTCTGCTAACTGCTT  >  NC_000913/2703871‑2704139

cATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCACCAGTTCCTGATGATTAAAGgtgt                                                                                                                         <  2:24291/150‑1 (MQ=255)

cATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGgtgt                                                                                                                         <  2:642252/150‑1 (MQ=255)

                aCGGTGATAAAGTGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGa                                                                                                        <  2:436912/151‑1 (MQ=255)

                        aaaGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGat                                                                                                 <  2:502765/150‑1 (MQ=255)

                        aaaGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGat                                                                                                 <  1:735328/150‑1 (MQ=255)

                         aaGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGatta                                                                                               <  1:168333/151‑1 (MQ=255)

                                   gCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATgg                                                                                     <  2:639020/151‑1 (MQ=255)

                                        aaCGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTc                                                                                <  1:600996/151‑1 (MQ=255)

                                           gTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCAt                                                                              <  2:30594/150‑1 (MQ=255)

                                             aGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGc                                                                            <  2:83751/150‑1 (MQ=255)

                                                  aGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATAcc                                                                      <  1:597127/151‑1 (MQ=255)

                                                  aGAATAGAGTCGCCTAAAAATTCTAAACACTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATAcc                                                                      >  2:539613/1‑151 (MQ=255)

                                                         agTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCaataaat                                                               <  2:370884/151‑1 (MQ=255)

                                                            cGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAataaataa                                                             <  1:467285/150‑1 (MQ=255)

                                                            cGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAataaataa                                                             <  1:243433/150‑1 (MQ=255)

                                                                   aaaTTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTca                                                     <  1:118646/151‑1 (MQ=255)

                                                                           aaCGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCa                                             <  1:106637/151‑1 (MQ=255)

                                                                             cGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGc                                           <  2:228141/151‑1 (MQ=255)

                                                                             cGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGc                                           <  2:279480/151‑1 (MQ=255)

                                                                                   ttATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACg                                      >  1:310105/1‑150 (MQ=255)

                                                                                       gTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACa                                 <  1:591793/151‑1 (MQ=255)

                                                                                       gTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACa                                 <  1:101241/151‑1 (MQ=255)

                                                                                          tACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGa                               <  1:273417/150‑1 (MQ=255)

                                                                                           aCTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGAcc                             <  1:29601/151‑1 (MQ=255)

                                                                                               ctgGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGACGCCGATTAATTACGATGGGGTTCTTGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACCAAACAGACCTGa                          <  2:207671/150‑1 (MQ=255)

                                                                                                     cTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGACCTGATATACa                    <  2:271527/150‑1 (MQ=255)

                                                                                                        cGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGACCTGATATACATGGt                <  2:315146/151‑1 (MQ=255)

                                                                                                                tAATGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGACCTGATATACATGGTTCTGCTa         <  2:80702/150‑1 (MQ=255)

                                                                                                                 aaTGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGACCTGATATACATGGTTCTGCTaa        <  1:539641/150‑1 (MQ=255)

                                                                                                                 aaTGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGACCTGATATACATGGTTCTGCTaa        <  1:103230/150‑1 (MQ=255)

                                                                                                                   tGCCTGCTGCAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGACCTGATATACATGGTTCTGCTAACTg     >  1:511332/1‑151 (MQ=255)

                                                                                                                        gctgcAACAGTTCCTGATGATTAAAGGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGACCTGATATACATGGTTCTGCTAACTGCtt  <  2:526/149‑1 (MQ=255)

CATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAAGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGACCTGATATACATGGTTCTGCTAACTGCTT  >  NC_000913/2703871‑2704139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A